Identification of cryptic sex chromosomes and isolation of X and Y borne genes

Paul Waters, Jenny Marshall Graves, Katherine Thompson, Natasha Sankovic, Tariq Ezaz

Research output: A Conference proceeding or a Chapter in BookChapter

3 Citations (Scopus)

Abstract

Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes
Original languageEnglish
Title of host publicationMethods in Molecular Biology: Phylogenomics
EditorsW.J Murphy
Place of PublicationUnited States of America
PublisherHumana Press
Pages239-251
Number of pages13
Edition422
ISBN (Electronic)9781597455817
ISBN (Print)9781588297648
DOIs
Publication statusPublished - 2008
Externally publishedYes

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Waters, P., Marshall Graves, J., Thompson, K., Sankovic, N., & Ezaz, T. (2008). Identification of cryptic sex chromosomes and isolation of X and Y borne genes. In W. J. Murphy (Ed.), Methods in Molecular Biology: Phylogenomics (422 ed., pp. 239-251). United States of America: Humana Press. https://doi.org/10.1007/978-1-59745-581-7_15