Identification of cryptic sex chromosomes and isolation of X and Y borne genes

Paul Waters, Jenny Marshall Graves, Katherine Thompson, Natasha Sankovic, Tariq Ezaz

Research output: A Conference proceeding or a Chapter in BookChapter

3 Citations (Scopus)

Abstract

Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes
Original languageEnglish
Title of host publicationMethods in Molecular Biology: Phylogenomics
EditorsW.J Murphy
Place of PublicationUnited States of America
PublisherHumana Press
Pages239-251
Number of pages13
Edition422
ISBN (Electronic)9781597455817
ISBN (Print)9781588297648
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Sex Chromosomes
Genes
Chromosome Banding
Reptiles
Y Chromosome
Cytogenetic Analysis
Cytogenetics
Birds
Vertebrates
Mammals
Clone Cells
Cell Culture Techniques
Chromosomes
History
Staining and Labeling

Cite this

Waters, P., Marshall Graves, J., Thompson, K., Sankovic, N., & Ezaz, T. (2008). Identification of cryptic sex chromosomes and isolation of X and Y borne genes. In W. J. Murphy (Ed.), Methods in Molecular Biology: Phylogenomics (422 ed., pp. 239-251). United States of America: Humana Press. https://doi.org/10.1007/978-1-59745-581-7_15
Waters, Paul ; Marshall Graves, Jenny ; Thompson, Katherine ; Sankovic, Natasha ; Ezaz, Tariq. / Identification of cryptic sex chromosomes and isolation of X and Y borne genes. Methods in Molecular Biology: Phylogenomics. editor / W.J Murphy. 422. ed. United States of America : Humana Press, 2008. pp. 239-251
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Waters, P, Marshall Graves, J, Thompson, K, Sankovic, N & Ezaz, T 2008, Identification of cryptic sex chromosomes and isolation of X and Y borne genes. in WJ Murphy (ed.), Methods in Molecular Biology: Phylogenomics. 422 edn, Humana Press, United States of America, pp. 239-251. https://doi.org/10.1007/978-1-59745-581-7_15

Identification of cryptic sex chromosomes and isolation of X and Y borne genes. / Waters, Paul; Marshall Graves, Jenny; Thompson, Katherine; Sankovic, Natasha; Ezaz, Tariq.

Methods in Molecular Biology: Phylogenomics. ed. / W.J Murphy. 422. ed. United States of America : Humana Press, 2008. p. 239-251.

Research output: A Conference proceeding or a Chapter in BookChapter

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Waters P, Marshall Graves J, Thompson K, Sankovic N, Ezaz T. Identification of cryptic sex chromosomes and isolation of X and Y borne genes. In Murphy WJ, editor, Methods in Molecular Biology: Phylogenomics. 422 ed. United States of America: Humana Press. 2008. p. 239-251 https://doi.org/10.1007/978-1-59745-581-7_15