Identification of cryptic sex chromosomes and isolation of X and Y borne genes

Paul Waters; Jenny Marshall Graves; Katherine Thompson; Natasha Sankovic; Tariq Ezaz

doi:10.1007/978-1-59745-581-7_15

Identification of cryptic sex chromosomes and isolation of X and Y borne genes

Paul Waters, Jenny Marshall Graves, Katherine Thompson, Natasha Sankovic, Tariq Ezaz

Research output: A Conference proceeding or a Chapter in Book › Chapter

3 Citations (Scopus)

Abstract

Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes

Original language	English
Title of host publication	Methods in Molecular Biology: Phylogenomics
Editors	W.J Murphy
Place of Publication	United States of America
Publisher	Humana Press
Pages	239-251
Number of pages	13
Edition	422
ISBN (Electronic)	9781597455817
ISBN (Print)	9781588297648
DOIs	https://doi.org/10.1007/978-1-59745-581-7_15
Publication status	Published - 2008
Externally published	Yes

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10.1007/978-1-59745-581-7_15

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abstract = "Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes",

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AU - Sankovic, Natasha

AU - Ezaz, Tariq

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AB - Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes

U2 - 10.1007/978-1-59745-581-7_15

DO - 10.1007/978-1-59745-581-7_15

M3 - Chapter

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BT - Methods in Molecular Biology: Phylogenomics

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ER -

Identification of cryptic sex chromosomes and isolation of X and Y borne genes

Abstract

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