Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

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    Abstract

    We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.
    Original languageEnglish
    Pages (from-to)733-735
    Number of pages3
    JournalConservation Genetics Resources
    Volume4
    Issue number3
    DOIs
    Publication statusPublished - 2012

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