Abstract
Original language | English |
---|---|
Pages (from-to) | 733-735 |
Number of pages | 3 |
Journal | Conservation Genetics Resources |
Volume | 4 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2012 |
Fingerprint
Cite this
}
Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid. / Sarre, Stephen.
In: Conservation Genetics Resources, Vol. 4, No. 3, 2012, p. 733-735.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid
AU - Sarre, Stephen
PY - 2012
Y1 - 2012
N2 - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.
AB - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.
KW - 454 Sequencing
KW - Microsatellites
KW - Pygopodidae
KW - Aprasia
KW - Australia.
U2 - 10.1007/s12686-012-9633-6
DO - 10.1007/s12686-012-9633-6
M3 - Article
VL - 4
SP - 733
EP - 735
JO - Conservation Genetics Resoures
JF - Conservation Genetics Resoures
SN - 1877-7252
IS - 3
ER -