Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

Stephen Sarre

doi:10.1007/s12686-012-9633-6

Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

Stephen Sarre

Science

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2 Citations (Scopus)

Abstract

We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

Original language	English
Pages (from-to)	733-735
Number of pages	3
Journal	Conservation Genetics Resources
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/s12686-012-9633-6
Publication status	Published - 2012

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title = "Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid",

abstract = "We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.",

keywords = "454 Sequencing, Microsatellites, Pygopodidae, Aprasia, Australia.",

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N2 - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

AB - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

KW - 454 Sequencing

KW - Microsatellites

KW - Pygopodidae

KW - Aprasia

KW - Australia.

U2 - 10.1007/s12686-012-9633-6

DO - 10.1007/s12686-012-9633-6

M3 - Article

VL - 4

SP - 733

EP - 735

JO - Conservation Genetics Resoures

JF - Conservation Genetics Resoures

SN - 1877-7252

IS - 3

ER -

Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

Abstract

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