Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

Stephen Sarre

    Research output: Contribution to journal › Article

    2 Citations (Scopus)

    Abstract

    We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.
    Original languageEnglish
    Pages (from-to)733-735
    Number of pages3
    JournalConservation Genetics Resources
    Volume4
    Issue number3
    DOIs
    Publication statusPublished - 2012

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    Lizards
    lizard
    Microsatellite Repeats
    lizards
    allele
    microsatellite repeats
    loci
    Alleles
    Population
    gene flow
    population structure
    polymorphism
    Gene Flow
    alleles
    marker
    genetic polymorphism
    testing
    test
    analysis

    Cite this

    Sarre, Stephen. / Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid. In: Conservation Genetics Resources. 2012 ; Vol. 4, No. 3. pp. 733-735.
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    abstract = "We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.",
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    Sarre, S 2012, 'Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid', Conservation Genetics Resources, vol. 4, no. 3, pp. 733-735. https://doi.org/10.1007/s12686-012-9633-6

    Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid. / Sarre, Stephen.

    In: Conservation Genetics Resources, Vol. 4, No. 3, 2012, p. 733-735.

    Research output: Contribution to journal › Article

    TY - JOUR

    T1 - Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

    AU - Sarre, Stephen

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    N2 - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

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    KW - Pygopodidae

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    Sarre S. Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid. Conservation Genetics Resources. 2012;4(3):733-735. https://doi.org/10.1007/s12686-012-9633-6

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