Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

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    Abstract

    We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.
    Original languageEnglish
    Pages (from-to)733-735
    Number of pages3
    JournalConservation Genetics Resources
    Volume4
    Issue number3
    DOIs
    Publication statusPublished - 2012

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    Lizards
    lizard
    Microsatellite Repeats
    lizards
    allele
    microsatellite repeats
    loci
    Alleles
    Population
    gene flow
    population structure
    polymorphism
    Gene Flow
    alleles
    marker
    genetic polymorphism
    testing
    test
    analysis

    Cite this

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    title = "Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid",
    abstract = "We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.",
    keywords = "454 Sequencing, Microsatellites, Pygopodidae, Aprasia, Australia.",
    author = "Stephen Sarre",
    year = "2012",
    doi = "10.1007/s12686-012-9633-6",
    language = "English",
    volume = "4",
    pages = "733--735",
    journal = "Conservation Genetics Resoures",
    issn = "1877-7252",
    publisher = "Springer Verlag",
    number = "3",

    }

    TY - JOUR

    T1 - Identification of microsatellite markers for the Pink-tailed Worm-lizard, Aprasia parapulchella (Kluge): an endangered pygopodid

    AU - Sarre, Stephen

    PY - 2012

    Y1 - 2012

    N2 - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

    AB - We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.

    KW - 454 Sequencing

    KW - Microsatellites

    KW - Pygopodidae

    KW - Aprasia

    KW - Australia.

    U2 - 10.1007/s12686-012-9633-6

    DO - 10.1007/s12686-012-9633-6

    M3 - Article

    VL - 4

    SP - 733

    EP - 735

    JO - Conservation Genetics Resoures

    JF - Conservation Genetics Resoures

    SN - 1877-7252

    IS - 3

    ER -