We used next-generation sequencing to develop 23 polymorphic microsatellites markers for the endangered Pink-tailed Worm-lizard, Aprasia parapulchella. Primers were developed for 68 loci from 3,430 microsatellite fragments identified. 25 loci were chosen to test for polymorphism, revealing an average of 4.87 alleles per locus and a total of 112 alleles in 53 individuals from two populations. Two loci were monomorphic. The loci obtained will enable the analysis of population structure and patterns of gene-flow in fragmented populations of this endemic legless lizard.