Identification of relaxin immunoreactivity in human follicular fluid

D. Claire Wathes; Peter G. Wardle; Jackie M. Rees; Josephine D. Mitchell; Eileen A. Mclaughlin; Michael G.R. Hull; David G. Porter

doi:10.1093/oxfordjournals.humrep.a136465

Identification of relaxin immunoreactivity in human follicular fluid

D. Claire Wathes, Peter G. Wardle, Jackie M. Rees, Josephine D. Mitchell, Eileen A. Mclaughlin, Michael G.R. Hull, David G. Porter

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

Original language	English
Pages (from-to)	515-517
Number of pages	3
Journal	Human Reproduction
Volume	1
Issue number	8
DOIs	https://doi.org/10.1093/oxfordjournals.humrep.a136465
Publication status	Published - 1 Dec 1986
Externally published	Yes

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title = "Identification of relaxin immunoreactivity in human follicular fluid",

abstract = "Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained{\~ } 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of{\~ } 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.",

keywords = "follicular fluid, hair follicle, ovarian follicle, relaxin, rupture, suidae, rats, uterus, ovulatory cycle",

author = "Wathes, {D. Claire} and Wardle, {Peter G.} and Rees, {Jackie M.} and Mitchell, {Josephine D.} and Mclaughlin, {Eileen A.} and Hull, {Michael G.R.} and Porter, {David G.}",

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doi = "10.1093/oxfordjournals.humrep.a136465",

language = "English",

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journal = "Human Reproduction",

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TY - JOUR

T1 - Identification of relaxin immunoreactivity in human follicular fluid

AU - Wathes, D. Claire

AU - Wardle, Peter G.

AU - Rees, Jackie M.

AU - Mitchell, Josephine D.

AU - Mclaughlin, Eileen A.

AU - Hull, Michael G.R.

AU - Porter, David G.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

AB - Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

KW - follicular fluid

KW - hair follicle

KW - ovarian follicle

KW - relaxin

KW - rupture

KW - suidae

KW - rats

KW - uterus

KW - ovulatory cycle

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