Identification of relaxin immunoreactivity in human follicular fluid

D. Claire Wathes, Peter G. Wardle, Jackie M. Rees, Josephine D. Mitchell, Eileen A. Mclaughlin, Michael G.R. Hull, David G. Porter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

Original languageEnglish
Pages (from-to)515-517
Number of pages3
JournalHuman Reproduction
Volume1
Issue number8
DOIs
Publication statusPublished - 1 Dec 1986
Externally publishedYes

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Relaxin
Follicular Fluid
Complex Mixtures
Biological Assay
Rupture
Swine

Cite this

Wathes, D. C., Wardle, P. G., Rees, J. M., Mitchell, J. D., Mclaughlin, E. A., Hull, M. G. R., & Porter, D. G. (1986). Identification of relaxin immunoreactivity in human follicular fluid. Human Reproduction, 1(8), 515-517. https://doi.org/10.1093/oxfordjournals.humrep.a136465
Wathes, D. Claire ; Wardle, Peter G. ; Rees, Jackie M. ; Mitchell, Josephine D. ; Mclaughlin, Eileen A. ; Hull, Michael G.R. ; Porter, David G. / Identification of relaxin immunoreactivity in human follicular fluid. In: Human Reproduction. 1986 ; Vol. 1, No. 8. pp. 515-517.
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Wathes, DC, Wardle, PG, Rees, JM, Mitchell, JD, Mclaughlin, EA, Hull, MGR & Porter, DG 1986, 'Identification of relaxin immunoreactivity in human follicular fluid', Human Reproduction, vol. 1, no. 8, pp. 515-517. https://doi.org/10.1093/oxfordjournals.humrep.a136465

Identification of relaxin immunoreactivity in human follicular fluid. / Wathes, D. Claire; Wardle, Peter G.; Rees, Jackie M.; Mitchell, Josephine D.; Mclaughlin, Eileen A.; Hull, Michael G.R.; Porter, David G.

In: Human Reproduction, Vol. 1, No. 8, 01.12.1986, p. 515-517.

Research output: Contribution to journalArticle

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Wathes DC, Wardle PG, Rees JM, Mitchell JD, Mclaughlin EA, Hull MGR et al. Identification of relaxin immunoreactivity in human follicular fluid. Human Reproduction. 1986 Dec 1;1(8):515-517. https://doi.org/10.1093/oxfordjournals.humrep.a136465