Identification of relaxin immunoreactivity in human follicular fluid

D. Claire Wathes; Peter G. Wardle; Jackie M. Rees; Josephine D. Mitchell; Eileen A. Mclaughlin; Michael G.R. Hull; David G. Porter

doi:10.1093/oxfordjournals.humrep.a136465

Identification of relaxin immunoreactivity in human follicular fluid

D. Claire Wathes, Peter G. Wardle, Jackie M. Rees, Josephine D. Mitchell, Eileen A. Mclaughlin, Michael G.R. Hull, David G. Porter

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Abstract

Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

Original language	English
Pages (from-to)	515-517
Number of pages	3
Journal	Human Reproduction
Volume	1
Issue number	8
DOIs	https://doi.org/10.1093/oxfordjournals.humrep.a136465
Publication status	Published - 1 Dec 1986
Externally published	Yes

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Wathes, D. C., Wardle, P. G., Rees, J. M., Mitchell, J. D., Mclaughlin, E. A., Hull, M. G. R., & Porter, D. G. (1986). Identification of relaxin immunoreactivity in human follicular fluid. Human Reproduction, 1(8), 515-517. https://doi.org/10.1093/oxfordjournals.humrep.a136465

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title = "Identification of relaxin immunoreactivity in human follicular fluid",

abstract = "Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60{\%} of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.",

keywords = "follicular fluid, hair follicle, ovarian follicle, relaxin, rupture, suidae, rats, uterus, ovulatory cycle",

author = "Wathes, {D. Claire} and Wardle, {Peter G.} and Rees, {Jackie M.} and Mitchell, {Josephine D.} and Mclaughlin, {Eileen A.} and Hull, {Michael G.R.} and Porter, {David G.}",

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T1 - Identification of relaxin immunoreactivity in human follicular fluid

AU - Wathes, D. Claire

AU - Wardle, Peter G.

AU - Rees, Jackie M.

AU - Mitchell, Josephine D.

AU - Mclaughlin, Eileen A.

AU - Hull, Michael G.R.

AU - Porter, David G.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

AB - Relaxin immunoreactivity has been found in samples of human follicular fluid collected from artificially stimulated pre-ovulatory follicles. The crude extract caused a reduction in the height of the contractions in a rat uterine strip bioassay. The reactive material eluted from Sephadex G50 in two major peaks. The first contained ̃ 60% of the immunoreactivity and had an elution position corresponding to that of porcine relaxin, indicating a mol. wt of ̃ 6000 daltons. The second peak was of a lower mol. wt, but its exact size and significance are unknown. A possible role for relaxin in the process of follicular rupture is suggested.

KW - follicular fluid

KW - hair follicle

KW - ovarian follicle

KW - relaxin

KW - rupture

KW - suidae

KW - rats

KW - uterus

KW - ovulatory cycle

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DO - 10.1093/oxfordjournals.humrep.a136465

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10.1093/oxfordjournals.humrep.a136465

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