IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages

Yan Li, Stephen Ohms, Mary Frances Shannon, Chao Sun, Jun Fan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

DNA demethylation has been found to occur at the promoters of a number of actively expressed cytokines and is believed to play a critical role in transcriptional regulation. While many DNA demethylation studies have focused on T cell activation, proliferation and differentiation, changes in DNA methylation in other types of immune cells are less well studied. We found that the expression of two cytokines (IL-2 and GM-CSF) responded differently to activation in three types of immune cells: EL4, A20 and RAW264.7 cells. Using the McrBC and MeDIP approaches, we observed decreases in DNA methylation at a genome-wide level and at the promoters of the genes of these cytokines. The expression of several potential enzymes/co-enzymes involved in the DNA demethylation pathways seemed to be associated with immune cell activation.
Original languageEnglish
Pages (from-to)748-753
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number4
DOIs
Publication statusPublished - 2012

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