IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages

Yan Li, Stephen Ohms, Mary Frances Shannon, Chao Sun, Jun Fan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

DNA demethylation has been found to occur at the promoters of a number of actively expressed cytokines and is believed to play a critical role in transcriptional regulation. While many DNA demethylation studies have focused on T cell activation, proliferation and differentiation, changes in DNA methylation in other types of immune cells are less well studied. We found that the expression of two cytokines (IL-2 and GM-CSF) responded differently to activation in three types of immune cells: EL4, A20 and RAW264.7 cells. Using the McrBC and MeDIP approaches, we observed decreases in DNA methylation at a genome-wide level and at the promoters of the genes of these cytokines. The expression of several potential enzymes/co-enzymes involved in the DNA demethylation pathways seemed to be associated with immune cell activation.
Original languageEnglish
Pages (from-to)748-753
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number4
DOIs
Publication statusPublished - 2012

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T-cells
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-2
B-Lymphocytes
Chemical activation
Cells
Cytokines
T-Lymphocytes
DNA
DNA Methylation
Genes
Enzymes
Cell Differentiation
Cell Proliferation
Genome

Cite this

Li, Yan ; Ohms, Stephen ; Shannon, Mary Frances ; Sun, Chao ; Fan, Jun. / IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 419, No. 4. pp. 748-753.
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IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. / Li, Yan; Ohms, Stephen; Shannon, Mary Frances; Sun, Chao; Fan, Jun.

In: Biochemical and Biophysical Research Communications, Vol. 419, No. 4, 2012, p. 748-753.

Research output: Contribution to journalArticle

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T1 - IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages

AU - Li, Yan

AU - Ohms, Stephen

AU - Shannon, Mary Frances

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AU - Fan, Jun

PY - 2012

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