DNA demethylation has been found to occur at the promoters of a number of actively expressed cytokines and is believed to play a critical role in transcriptional regulation. While many DNA demethylation studies have focused on T cell activation, proliferation and differentiation, changes in DNA methylation in other types of immune cells are less well studied. We found that the expression of two cytokines (IL-2 and GM-CSF) responded differently to activation in three types of immune cells: EL4, A20 and RAW264.7 cells. Using the McrBC and MeDIP approaches, we observed decreases in DNA methylation at a genome-wide level and at the promoters of the genes of these cytokines. The expression of several potential enzymes/co-enzymes involved in the DNA demethylation pathways seemed to be associated with immune cell activation.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2012|
Li, Y., Ohms, S., Shannon, M. F., Sun, C., & Fan, J. (2012). IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. Biochemical and Biophysical Research Communications, 419(4), 748-753. https://doi.org/10.1016/j.bbrc.2012.02.094