Immobilized chiral selectors on monolithic high-performance liquid chromatography columns

Ali Fouad, Ashraf GHANEM

Research output: A Conference proceeding or a Chapter in BookChapterpeer-review

3 Citations (Scopus)


This chapter summarizes the recent development of chiral selectors (CSs) immobilization on high-performance liquid chromatography (HPLC) monolithic stationary phases. Three main classes of monolithic stationary phases have been reported, namely organic polymer-based, silica-based, and hybrid monolithic stationary phases. Most enantioselective separations by HPLC are done via direct resolution with a chiral stationary phase (CSP) where the chiral resolving agent is adsorbed, attached, bound, or immobilized to an appropriate support to make a CSP. The enantiomers are resolved by the formation of temporary diastereomeric complexes between the analyte and the CSP. With the development of enantioselective chromatography, many CSs such as polysaccharides, crown ethers, antibiotics have been introduced and documented as potent CSPs for the resolution of racemates. The mechanism of immobilization of CSs in stationary phases varied in between particles as immobilization, functionalization, coating, and bonding to monolithic column or capillary.
Original languageEnglish
Title of host publicationAdvances in Chromatography
Subtitle of host publicationVolume 54
EditorsEli Grushka, Nelu Grinberg
Place of PublicationBoca Raton, USA
PublisherCRC Press
Number of pages58
ISBN (Electronic)9781351652971
ISBN (Print)9781138055957
Publication statusPublished - Jul 2017

Publication series

NameAdvances in Chromatography
ISSN (Print)0065-2415


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