TY - CHAP
T1 - Immobilized chiral selectors on monolithic high-performance liquid chromatography columns
AU - Fouad, Ali
AU - GHANEM, Ashraf
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2017/7
Y1 - 2017/7
N2 - This chapter summarizes the recent development of chiral selectors (CSs) immobilization on high-performance liquid chromatography (HPLC) monolithic stationary phases. Three main classes of monolithic stationary phases have been reported, namely organic polymer-based, silica-based, and hybrid monolithic stationary phases. Most enantioselective separations by HPLC are done via direct resolution with a chiral stationary phase (CSP) where the chiral resolving agent is adsorbed, attached, bound, or immobilized to an appropriate support to make a CSP. The enantiomers are resolved by the formation of temporary diastereomeric complexes between the analyte and the CSP. With the development of enantioselective chromatography, many CSs such as polysaccharides, crown ethers, antibiotics have been introduced and documented as potent CSPs for the resolution of racemates. The mechanism of immobilization of CSs in stationary phases varied in between particles as immobilization, functionalization, coating, and bonding to monolithic column or capillary.
AB - This chapter summarizes the recent development of chiral selectors (CSs) immobilization on high-performance liquid chromatography (HPLC) monolithic stationary phases. Three main classes of monolithic stationary phases have been reported, namely organic polymer-based, silica-based, and hybrid monolithic stationary phases. Most enantioselective separations by HPLC are done via direct resolution with a chiral stationary phase (CSP) where the chiral resolving agent is adsorbed, attached, bound, or immobilized to an appropriate support to make a CSP. The enantiomers are resolved by the formation of temporary diastereomeric complexes between the analyte and the CSP. With the development of enantioselective chromatography, many CSs such as polysaccharides, crown ethers, antibiotics have been introduced and documented as potent CSPs for the resolution of racemates. The mechanism of immobilization of CSs in stationary phases varied in between particles as immobilization, functionalization, coating, and bonding to monolithic column or capillary.
KW - Capillary Electrochromatography
KW - Monolithic integrated circuits
KW - polymer monoliths
UR - http://www.scopus.com/inward/record.url?scp=85053953916&partnerID=8YFLogxK
U2 - 10.1201/9781315116372
DO - 10.1201/9781315116372
M3 - Chapter
AN - SCOPUS:85053953916
SN - 9781138055957
VL - 54
T3 - Advances in Chromatography
SP - 111
EP - 168
BT - Advances in Chromatography
A2 - Grushka, Eli
A2 - Grinberg, Nelu
PB - CRC Press
CY - Boca Raton, USA
ER -