This chapter summarizes the recent development of chiral selectors (CSs) immobilization on high-performance liquid chromatography (HPLC) monolithic stationary phases. Three main classes of monolithic stationary phases have been reported, namely organic polymer-based, silica-based, and hybrid monolithic stationary phases. Most enantioselective separations by HPLC are done via direct resolution with a chiral stationary phase (CSP) where the chiral resolving agent is adsorbed, attached, bound, or immobilized to an appropriate support to make a CSP. The enantiomers are resolved by the formation of temporary diastereomeric complexes between the analyte and the CSP. With the development of enantioselective chromatography, many CSs such as polysaccharides, crown ethers, antibiotics have been introduced and documented as potent CSPs for the resolution of racemates. The mechanism of immobilization of CSs in stationary phases varied in between particles as immobilization, functionalization, coating, and bonding to monolithic column or capillary.
|Title of host publication||Advances in Chromatography|
|Subtitle of host publication||Volume 54|
|Editors||Eli Grushka, Nelu Grinberg|
|Place of Publication||Boca Raton, USA|
|Number of pages||58|
|Publication status||Published - 15 Aug 2017|
|Name||Advances in Chromatography|