Immunoelectrophoretic analysis of Streptococcus agalactiae serotype Ia antigens

S. M. Somerset, G. H G Davis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rabbit antibodies to heat-killed whole cells of Streptococcus agalactiae serotype Ia were used to establish an antigen map using Triton X-100 sonicates of homologous cells and crossed immunoelectrophoresis. A total of 11 antigens were identified but the density of immunoprecipitates was varied and only seven could be reliably detected, one of which dominated the immunoprecipitate pattern by its intensity. The antigens were partially characterized by immunological, chemical and cell-location methods. Five of the antigens contained carbohydrate and two of those were sensitive to trypsin and probably represent cell-wall compounds. Of the three most prominent antigens, one was surface located and represented the type and shared type antigens (Iabc), one was a cell-wall carbohydrate and very sensitive to periodate, and one was a protein/carbohydrate complex which was heat-labile and trypsin sensitive. Group B epitopes were detected in three immunoprecipitates. Cross-reactions between type Ia and other serotypes and streptococci were recorded.

Original languageEnglish
Pages (from-to)633-640
Number of pages8
JournalJournal of General Microbiology
Volume132
Issue number3
Publication statusPublished - 1 Jan 1986
Externally publishedYes

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Streptococcus agalactiae
Histocompatibility Antigens Class II
Antigens
Carbohydrates
Trypsin
Cell Wall
Hot Temperature
Two-Dimensional Immunoelectrophoresis
Cross Reactions
Octoxynol
Streptococcus
Serogroup
Epitopes
Rabbits
Antibodies
Proteins

Cite this

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abstract = "Rabbit antibodies to heat-killed whole cells of Streptococcus agalactiae serotype Ia were used to establish an antigen map using Triton X-100 sonicates of homologous cells and crossed immunoelectrophoresis. A total of 11 antigens were identified but the density of immunoprecipitates was varied and only seven could be reliably detected, one of which dominated the immunoprecipitate pattern by its intensity. The antigens were partially characterized by immunological, chemical and cell-location methods. Five of the antigens contained carbohydrate and two of those were sensitive to trypsin and probably represent cell-wall compounds. Of the three most prominent antigens, one was surface located and represented the type and shared type antigens (Iabc), one was a cell-wall carbohydrate and very sensitive to periodate, and one was a protein/carbohydrate complex which was heat-labile and trypsin sensitive. Group B epitopes were detected in three immunoprecipitates. Cross-reactions between type Ia and other serotypes and streptococci were recorded.",
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Immunoelectrophoretic analysis of Streptococcus agalactiae serotype Ia antigens. / Somerset, S. M.; Davis, G. H G.

In: Journal of General Microbiology, Vol. 132, No. 3, 01.01.1986, p. 633-640.

Research output: Contribution to journalArticle

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