Eleven antigen extraction treatments were tested for their ability to release intact the seven antigens of Streptococcus agalactiae serotype 1a which are readily detected by Crossed immunoelectrophoresis (Crossed IEP). Treatments intended for the extraction of diagnostically important antigens (i.e. the group B- and serotype-specific antigens) result in the destruction of most of the antigens detectable by Crossed IEP. All but two antigens were destroyed by Lancefield acid/heat, Pattison mild heat, formamide and formalin treatments. All except one antigen were destroyed by autoclave, nitrous acid and autoclave/pronase treatments. Sonication of either live or heat-killed cells in the presence of Triton X-100 or a mild buffer wash of live cells yielded the widest range of antigens.
|Number of pages||5|
|Publication status||Published - 1 Jan 1986|