Immunoelectrophoretic comparison of streptococcal antigen extraction methods

S. M. Somerset, G. H.G. Davis

Research output: Contribution to journalArticle

Abstract

Eleven antigen extraction treatments were tested for their ability to release intact the seven antigens of Streptococcus agalactiae serotype 1a which are readily detected by Crossed immunoelectrophoresis (Crossed IEP). Treatments intended for the extraction of diagnostically important antigens (i.e. the group B- and serotype-specific antigens) result in the destruction of most of the antigens detectable by Crossed IEP. All but two antigens were destroyed by Lancefield acid/heat, Pattison mild heat, formamide and formalin treatments. All except one antigen were destroyed by autoclave, nitrous acid and autoclave/pronase treatments. Sonication of either live or heat-killed cells in the presence of Triton X-100 or a mild buffer wash of live cells yielded the widest range of antigens.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalMicrobios Letters
Volume33
Issue number131-132
Publication statusPublished - 1 Jan 1986
Externally publishedYes

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Antigens
Two-Dimensional Immunoelectrophoresis
Hot Temperature
Autoclaves
Nitrous Acid
Pronase
Sonication
Streptococcus agalactiae
Octoxynol
Formaldehyde
Buffers
Acids
Serogroup
Immunoelectrophoresis

Cite this

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Immunoelectrophoretic comparison of streptococcal antigen extraction methods. / Somerset, S. M.; Davis, G. H.G.

In: Microbios Letters, Vol. 33, No. 131-132, 01.01.1986, p. 143-147.

Research output: Contribution to journalArticle

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