Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium

Michelle Gahan, Diane E. Webster, Steven Wesselingh, Strugnell

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses
Original languageEnglish
Pages (from-to)1476-1483
Number of pages8
JournalVaccine
Volume25
Issue number8
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Salmonella enterica
Salmonella Typhimurium
mouth
plasmids
Plasmids
DNA
tetanus
Cytomegalovirus
Tetanus Toxoid
DNA Vaccines
toxoids
recombinant vaccines
humoral immunity
Salmonella
Antibody Formation
Serogroup
Immunization
immunization
toxins
Vaccines

Cite this

Gahan, Michelle ; Webster, Diane E. ; Wesselingh, Steven ; Strugnell. / Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium. In: Vaccine. 2007 ; Vol. 25, No. 8. pp. 1476-1483.
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Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium. / Gahan, Michelle; Webster, Diane E.; Wesselingh, Steven; Strugnell.

In: Vaccine, Vol. 25, No. 8, 2007, p. 1476-1483.

Research output: Contribution to journalArticle

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AB - Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses

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