Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1476-1483 |
| Number of pages | 8 |
| Journal | Vaccine |
| Volume | 25 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2007 |
| Externally published | Yes |
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Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium. / Gahan, Michelle; Webster, Diane E.; Wesselingh, Steven; Strugnell.
In: Vaccine, Vol. 25, No. 8, 2007, p. 1476-1483.Research output: Contribution to journal › Article
TY - JOUR
T1 - Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium
AU - Gahan, Michelle
AU - Webster, Diane E.
AU - Wesselingh, Steven
AU - Strugnell, null
PY - 2007
Y1 - 2007
N2 - Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses
AB - Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses
U2 - 10.1016/j.vaccine.2006.10.042
DO - 10.1016/j.vaccine.2006.10.042
M3 - Article
VL - 25
SP - 1476
EP - 1483
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 8
ER -