Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium

Michelle Gahan; Diane E. Webster; Steven Wesselingh; null Strugnell

doi:10.1016/j.vaccine.2006.10.042

Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium

Michelle Gahan, Diane E. Webster, Steven Wesselingh, Strugnell

Research output: Contribution to journal › Article

25 Citations (Scopus)

Abstract

Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses

Original language	English
Pages (from-to)	1476-1483
Number of pages	8
Journal	Vaccine
Volume	25
Issue number	8
DOIs	https://doi.org/10.1016/j.vaccine.2006.10.042
Publication status	Published - 2007
Externally published	Yes

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Gahan, M., Webster, D. E., Wesselingh, S., & Strugnell (2007). Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium. Vaccine, 25(8), 1476-1483. https://doi.org/10.1016/j.vaccine.2006.10.042

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title = "Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium",

abstract = "Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses",

author = "Michelle Gahan and Webster, {Diane E.} and Steven Wesselingh and Strugnell",

year = "2007",

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AU - Gahan, Michelle

AU - Webster, Diane E.

AU - Wesselingh, Steven

AU - Strugnell, null

PY - 2007

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N2 - Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses

AB - Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses

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DO - 10.1016/j.vaccine.2006.10.042

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10.1016/j.vaccine.2006.10.042