Improving reliability in environmental DNA detection surveys through enhanced quality control

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Species-specific environmentalDNA(eDNA) surveys are increasingly being used to infer species presence in an environment. Current inadequacies in quality control increase concern for false negatives, which can have serious ramifications for both the management of invasive species and the conservation of native species. eDNA surveys involve a multi-step process to sample, capture, extract and amplify target DNA from the environment. We outline various positive control options and show that many of the commonly used controls are capable of detecting false negatives arising during the amplification stage only. We suggest a secondary, generic primer, designed to co-amplify endogenous DNA sampled during species-specific eDNA surveys, constitutes a superior positive control to monitor method success throughout all stages ofeDNAanalysis.Wedevelop a species-specific European carp (Cyprinus carpio) assay and a generic fish assay for use as an endogenous control for eDNA surveys in Australian freshwater systems where fish are known to be abundant.We use these assays in a multiplex on eDNA samples that are simultaneously sampled, captured, extracted and amplified. This positive control allows us to distinguish method error from informative non-amplification results, improving reliability in eDNA surveys, which will ultimately lead to better informed conservation management decisions. Journal compilation.

    Original languageEnglish
    Pages (from-to)388-395
    Number of pages8
    JournalMarine and Freshwater Research
    Volume68
    Issue number2
    DOIs
    Publication statusPublished - 2017

    Fingerprint Dive into the research topics of 'Improving reliability in environmental DNA detection surveys through enhanced quality control'. Together they form a unique fingerprint.

  • Cite this