In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises

Geneviève Laublin; Hargurdeep S. Saini; Mario Cappadocia

doi:10.1007/BF00048200

In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises

Geneviève Laublin, Hargurdeep S. Saini, Mario Cappadocia

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Abstract

A method for plant regeneration of Iris via somatic embryogenesis is described. Root and leaf pieces from in vitro-grown plants of several genotypes of rhizomatous Iris sp. were cultured in vitro. Callus induction occurred only on root cultures incubated under low light intensity (35 μmol m^-2 s^-1) on two induction media containing 2,4-D (4.5 or 22.5 μM), NAA (5.4 μM) and kinetin (0.5 μM). Somatic embryos developed after transfer of callus onto four regeneration media containing 9 or 22 μM BA, or 5 μM kinetin and 2 μM TIBA or 9 μM BA and 4 μM TIBA. Plantlets could be obtained from these somatic embryos. Genotypic differences were found both in callus induction and somatic embryo formation, with I. pseudacorus responding better than I. versicolor or I. setosa. Cytological analysis performed on root tips of 80 regenerated plants revealed that two of the I. pseudacorus regenerants were tetraploid.

Original language	English
Pages (from-to)	15-21
Number of pages	7
Journal	Plant Cell, Tissue and Organ Culture
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/BF00048200
Publication status	Published - 1 Oct 1991
Externally published	Yes

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title = "In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises",

abstract = "A method for plant regeneration of Iris via somatic embryogenesis is described. Root and leaf pieces from in vitro-grown plants of several genotypes of rhizomatous Iris sp. were cultured in vitro. Callus induction occurred only on root cultures incubated under low light intensity (35 μmol m-2 s-1) on two induction media containing 2,4-D (4.5 or 22.5 μM), NAA (5.4 μM) and kinetin (0.5 μM). Somatic embryos developed after transfer of callus onto four regeneration media containing 9 or 22 μM BA, or 5 μM kinetin and 2 μM TIBA or 9 μM BA and 4 μM TIBA. Plantlets could be obtained from these somatic embryos. Genotypic differences were found both in callus induction and somatic embryo formation, with I. pseudacorus responding better than I. versicolor or I. setosa. Cytological analysis performed on root tips of 80 regenerated plants revealed that two of the I. pseudacorus regenerants were tetraploid.",

keywords = "I. setosa, I. versicolor, in vitro, Iris pseudacorus, root culture, somatic embryogenesis",

author = "Genevi{\`e}ve Laublin and Saini, {Hargurdeep S.} and Mario Cappadocia",

year = "1991",

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doi = "10.1007/BF00048200",

language = "English",

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AU - Laublin, Geneviève

AU - Saini, Hargurdeep S.

AU - Cappadocia, Mario

PY - 1991/10/1

Y1 - 1991/10/1

N2 - A method for plant regeneration of Iris via somatic embryogenesis is described. Root and leaf pieces from in vitro-grown plants of several genotypes of rhizomatous Iris sp. were cultured in vitro. Callus induction occurred only on root cultures incubated under low light intensity (35 μmol m-2 s-1) on two induction media containing 2,4-D (4.5 or 22.5 μM), NAA (5.4 μM) and kinetin (0.5 μM). Somatic embryos developed after transfer of callus onto four regeneration media containing 9 or 22 μM BA, or 5 μM kinetin and 2 μM TIBA or 9 μM BA and 4 μM TIBA. Plantlets could be obtained from these somatic embryos. Genotypic differences were found both in callus induction and somatic embryo formation, with I. pseudacorus responding better than I. versicolor or I. setosa. Cytological analysis performed on root tips of 80 regenerated plants revealed that two of the I. pseudacorus regenerants were tetraploid.

AB - A method for plant regeneration of Iris via somatic embryogenesis is described. Root and leaf pieces from in vitro-grown plants of several genotypes of rhizomatous Iris sp. were cultured in vitro. Callus induction occurred only on root cultures incubated under low light intensity (35 μmol m-2 s-1) on two induction media containing 2,4-D (4.5 or 22.5 μM), NAA (5.4 μM) and kinetin (0.5 μM). Somatic embryos developed after transfer of callus onto four regeneration media containing 9 or 22 μM BA, or 5 μM kinetin and 2 μM TIBA or 9 μM BA and 4 μM TIBA. Plantlets could be obtained from these somatic embryos. Genotypic differences were found both in callus induction and somatic embryo formation, with I. pseudacorus responding better than I. versicolor or I. setosa. Cytological analysis performed on root tips of 80 regenerated plants revealed that two of the I. pseudacorus regenerants were tetraploid.

KW - I. setosa

KW - I. versicolor

KW - in vitro

KW - Iris pseudacorus

KW - root culture

KW - somatic embryogenesis

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DO - 10.1007/BF00048200

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JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 1

ER -

In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises

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