In vitro sensitivity of the amphibian pathogen Batrachochytrium dendrobatidis to antifungal therapeutics

A. Woodward, L. Berger, L. F. Skerratt

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Chytridiomycosis, a skin disease caused by Batrachochytrium dendrobatidis, has caused amphibian declines worldwide. Amphibians can be treated by percutaneous application of antimicrobials, but knowledge of in vitro susceptibility is lacking. Using a modified broth microdilution method, we describe the in vitro sensitivity of two Australian isolates of B. dendrobatidis to six antimicrobial agents. Growth inhibition was observed, by measurement of optical density, with all agents. Minimum inhibitory concentrations (μg/ml; isolate 1/2) were - voriconazole 0.016/0.008; itraconazole 0.032/0.016; terbinafine 0.063/ 0.063; fluconazole 0.31/0.31; chloramphenicol 12.5/12.5; amphotericin B 12.5/6.25. Killing effects on zoospores were assessed by observing motility. Amphotericin B and terbinafine killed zoospores within 5 and 30 min depending on concentration, but other antimicrobials were not effective at the highest concentrations tested (100 μg/ml). This knowledge will help in drug selection and treatment optimization. As terbinafine was potent and has rapid effects, study of its pharmacokinetics, safety and efficacy is recommended.

Original languageEnglish
Pages (from-to)365-367
Number of pages3
JournalResearch in Veterinary Science
Volume97
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

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