Incubation Time And Its Effect On Sperm Motility Following Cryopreservation

Lachlan Campbell; Rose Upton; Sean Doody; John Clulow; Simon Clulow

doi:10.1016/j.cryobiol.2019.10.039

Abstract

The yellow-spotted monitor (Varanus panoptes) is an Australian lizard species which is severely impacted by the invasive cane toad. Previous attempts at sperm cryopreservation have shown promising post-thaw survival (∼ 45% motility), however the length of incubation time prior to cryopreservation has not been tested for this species and is important due to the remote locality of field sites. The aim of this study was to determine the time in which sperm can maintain motility before cryopreservation and whether this affects post-thaw sperm survival. The sperm of each animal was macerated into 1 mL PBS (Ca2+, Mg+-free), and the supernatant was collected after one minute.

Original language	English
Pages (from-to)	154-154
Number of pages	1
Journal	Cryobiology
Volume	91
DOIs	https://doi.org/10.1016/j.cryobiol.2019.10.039
Publication status	Published - Dec 2019
Externally published	Yes
Event	CRYO2019: 56th Annual Meeting of the Society for Cryobiology - San Diego, San Diego, United States Duration: 22 Jul 2019 → 25 Jul 2019

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10.1016/j.cryobiol.2019.10.039

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title = "Incubation Time And Its Effect On Sperm Motility Following Cryopreservation",

abstract = "The yellow-spotted monitor (Varanus panoptes) is an Australian lizard species which is severely impacted by the invasive cane toad. Previous attempts at sperm cryopreservation have shown promising post-thaw survival (∼ 45% motility), however the length of incubation time prior to cryopreservation has not been tested for this species and is important due to the remote locality of field sites. The aim of this study was to determine the time in which sperm can maintain motility before cryopreservation and whether this affects post-thaw sperm survival. The sperm of each animal was macerated into 1 mL PBS (Ca2+, Mg+-free), and the supernatant was collected after one minute.",

author = "Lachlan Campbell and Rose Upton and Sean Doody and John Clulow and Simon Clulow",

year = "2019",

month = dec,

doi = "10.1016/j.cryobiol.2019.10.039",

language = "English",

volume = "91",

pages = "154--154",

journal = "Cryobiology",

issn = "0011-2240",

publisher = "Academic Press Inc.",

note = "CRYO2019: 56th Annual Meeting of the Society for Cryobiology, CRYO2019 ; Conference date: 22-07-2019 Through 25-07-2019",

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T1 - Incubation Time And Its Effect On Sperm Motility Following Cryopreservation

AU - Campbell, Lachlan

AU - Upton, Rose

AU - Doody, Sean

AU - Clulow, John

AU - Clulow, Simon

PY - 2019/12

Y1 - 2019/12

N2 - The yellow-spotted monitor (Varanus panoptes) is an Australian lizard species which is severely impacted by the invasive cane toad. Previous attempts at sperm cryopreservation have shown promising post-thaw survival (∼ 45% motility), however the length of incubation time prior to cryopreservation has not been tested for this species and is important due to the remote locality of field sites. The aim of this study was to determine the time in which sperm can maintain motility before cryopreservation and whether this affects post-thaw sperm survival. The sperm of each animal was macerated into 1 mL PBS (Ca2+, Mg+-free), and the supernatant was collected after one minute.

AB - The yellow-spotted monitor (Varanus panoptes) is an Australian lizard species which is severely impacted by the invasive cane toad. Previous attempts at sperm cryopreservation have shown promising post-thaw survival (∼ 45% motility), however the length of incubation time prior to cryopreservation has not been tested for this species and is important due to the remote locality of field sites. The aim of this study was to determine the time in which sperm can maintain motility before cryopreservation and whether this affects post-thaw sperm survival. The sperm of each animal was macerated into 1 mL PBS (Ca2+, Mg+-free), and the supernatant was collected after one minute.

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U2 - 10.1016/j.cryobiol.2019.10.039

DO - 10.1016/j.cryobiol.2019.10.039

M3 - Meeting Abstract

SN - 0011-2240

VL - 91

SP - 154

EP - 154

JO - Cryobiology

JF - Cryobiology

T2 - CRYO2019: 56th Annual Meeting of the Society for Cryobiology

Y2 - 22 July 2019 through 25 July 2019

ER -

Incubation Time And Its Effect On Sperm Motility Following Cryopreservation

Abstract

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