Incubation Time And Its Effect On Sperm Motility Following Cryopreservation

Lachlan Campbell, Rose Upton, Sean Doody, John Clulow, Simon Clulow

Research output: Contribution to journalMeeting Abstractpeer-review


The yellow-spotted monitor (Varanus panoptes) is an Australian lizard species which is severely impacted by the invasive cane toad. Previous attempts at sperm cryopreservation have shown promising post-thaw survival (∼ 45% motility), however the length of incubation time prior to cryopreservation has not been tested for this species and is important due to the remote locality of field sites. The aim of this study was to determine the time in which sperm can maintain motility before cryopreservation and whether this affects post-thaw sperm survival. The sperm of each animal was macerated into 1 mL PBS (Ca2+, Mg+-free), and the supernatant was collected after one minute.
Original languageEnglish
Pages (from-to)154-154
Number of pages1
Publication statusPublished - Dec 2019
Externally publishedYes
EventCRYO2019: 56th Annual Meeting of the Society for Cryobiology - San Diego, San Diego, United States
Duration: 22 Jul 201925 Jul 2019


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