Independent Evolution of Transcriptional Inactivation on Sex Chromosomes in Birds and Mammals

Alexandra Livernois, Shafagh Waters, Janine Deakin, Jennifer Marshall Graves, Paul Waters

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Abstract

X chromosome inactivation in eutherian mammals has been thought to be tightly controlled, as expected from a mechanism that compensates for the different dosage of X-borne genes in XX females and XY males. However, many X genes escape inactivation in humans, inactivation of the X in marsupials is partial, and the unrelated sex chromosomes of monotreme mammals have incomplete and gene-specific inactivation of X-linked genes. The bird ZW sex chromosome system represents a third independently evolved amniote sex chromosome system with dosage compensation, albeit partial and gene-specific, via an unknown mechanism (i.e. upregulation of the single Z in females, down regulation of one or both Zs in males, or a combination). We used RNA-fluorescent in situ hybridization (RNA-FISH) to demonstrate, on individual fibroblast cells, inactivation of 11 genes on the chicken Z and 28 genes on the X chromosomes of platypus. Each gene displayed a reproducible frequency of 1Z/1X-active and 2Z/2X-active cells in the homogametic sex. Our results indicate that the probability of inactivation is controlled on a gene-by-gene basis (or small domains) on the chicken Z and platypus X chromosomes. This regulatory mechanism must have been exapted independently to the non-homologous sex chromosomes in birds and mammals in response to an over-expressed Z or X in the homogametic sex, highlighting the universal importance that (at least partial) silencing plays in the evolution on amniote dosage compensation and, therefore, the differentiation of sex chromosomes.
Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPLoS Genetics
Volume9
Issue number7
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Sex Chromosomes
sex chromosomes
Birds
Mammals
chromosome
inactivation
mammal
mammals
bird
Gene Silencing
Platypus
gene
birds
X Chromosome Inactivation
X-Linked Genes
Genes
genes
Chickens
X chromosome
Marsupialia

Cite this

Livernois, Alexandra ; Waters, Shafagh ; Deakin, Janine ; Marshall Graves, Jennifer ; Waters, Paul. / Independent Evolution of Transcriptional Inactivation on Sex Chromosomes in Birds and Mammals. In: PLoS Genetics. 2013 ; Vol. 9, No. 7. pp. 1-9.
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Independent Evolution of Transcriptional Inactivation on Sex Chromosomes in Birds and Mammals. / Livernois, Alexandra; Waters, Shafagh; Deakin, Janine; Marshall Graves, Jennifer; Waters, Paul.

In: PLoS Genetics, Vol. 9, No. 7, 2013, p. 1-9.

Research output: Contribution to journalArticle

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