Influence of culture regime on arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and Thalassiosira pseudonana

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    Abstract

    Arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and the marine diatom Thalassiosira pseudonana was influenced by culture regime. Arsenic was associated with the residue cell fractions of batch cultured phytoplankton (D. tertiolecta and T. pseudonana), due to the accumulation of dead cells within batch cultures. Greater arsenic concentrations were associated with water-soluble and lipid- oluble cell fractions of continuously cultured phytoplankton. Arsenoribosides (as glycerol (Gly-), phosphate (PO4-) and sulfate (OSO3-)) were ubiquitous in D. tertiolecta (Gly- and PO4- only) and T. pseudonana (all three species). Additionally, arsenobetaine (AB) was not detected in any phytoplankton tissues, illustrating that marine phytoplankton themselves are not an alternate source of AB. Arsenic species formation was influenced by culture regime, with PO4-riboside produced under nutrient rich conditions, whereas Dimethylarsenoacetate (DMAA) was found in old (.42 days old) batch cultures, with this arsenic species possibly produced by the degradation of arsenoribosides-arsenolipids from decomposing cells rather than by biosynthesis. Nutrient availability, hence culture regime was thus influential in directly and indirectly influencing arsenic cycling and the arsenic species produced by D. tertiolecta and T. pseudonana. Future research should thus utilise continuous culture regimes to study arsenic cycling as these are far more analogous to environmental processes.
    Original languageEnglish
    Pages (from-to)91-101
    Number of pages11
    JournalEnvironmental Chemistry
    Volume10
    DOIs
    Publication statusPublished - 2013

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    Phytoplankton
    Arsenic
    arsenic
    phytoplankton
    Glycerol
    Nutrients
    Batch cell culture
    Biosynthesis
    nutrient availability
    Sulfates
    diatom
    lipid
    Phosphates
    phosphate
    Availability
    Tissue
    sulfate
    Lipids
    Degradation
    degradation

    Cite this

    @article{af96e852cd084d3abe06d57421f316b3,
    title = "Influence of culture regime on arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and Thalassiosira pseudonana",
    abstract = "Arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and the marine diatom Thalassiosira pseudonana was influenced by culture regime. Arsenic was associated with the residue cell fractions of batch cultured phytoplankton (D. tertiolecta and T. pseudonana), due to the accumulation of dead cells within batch cultures. Greater arsenic concentrations were associated with water-soluble and lipid- oluble cell fractions of continuously cultured phytoplankton. Arsenoribosides (as glycerol (Gly-), phosphate (PO4-) and sulfate (OSO3-)) were ubiquitous in D. tertiolecta (Gly- and PO4- only) and T. pseudonana (all three species). Additionally, arsenobetaine (AB) was not detected in any phytoplankton tissues, illustrating that marine phytoplankton themselves are not an alternate source of AB. Arsenic species formation was influenced by culture regime, with PO4-riboside produced under nutrient rich conditions, whereas Dimethylarsenoacetate (DMAA) was found in old (.42 days old) batch cultures, with this arsenic species possibly produced by the degradation of arsenoribosides-arsenolipids from decomposing cells rather than by biosynthesis. Nutrient availability, hence culture regime was thus influential in directly and indirectly influencing arsenic cycling and the arsenic species produced by D. tertiolecta and T. pseudonana. Future research should thus utilise continuous culture regimes to study arsenic cycling as these are far more analogous to environmental processes.",
    keywords = "arsenic species, arsenoribosides, batch culture, continuous culture, lipid-soluble arsenic.",
    author = "Bill MAHER and Simon FOSTER",
    year = "2013",
    doi = "10.1071/EN12191",
    language = "English",
    volume = "10",
    pages = "91--101",
    journal = "Environmental Chemistry",
    issn = "1448-2517",
    publisher = "CSIRO",

    }

    TY - JOUR

    T1 - Influence of culture regime on arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and Thalassiosira pseudonana

    AU - MAHER, Bill

    AU - FOSTER, Simon

    PY - 2013

    Y1 - 2013

    N2 - Arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and the marine diatom Thalassiosira pseudonana was influenced by culture regime. Arsenic was associated with the residue cell fractions of batch cultured phytoplankton (D. tertiolecta and T. pseudonana), due to the accumulation of dead cells within batch cultures. Greater arsenic concentrations were associated with water-soluble and lipid- oluble cell fractions of continuously cultured phytoplankton. Arsenoribosides (as glycerol (Gly-), phosphate (PO4-) and sulfate (OSO3-)) were ubiquitous in D. tertiolecta (Gly- and PO4- only) and T. pseudonana (all three species). Additionally, arsenobetaine (AB) was not detected in any phytoplankton tissues, illustrating that marine phytoplankton themselves are not an alternate source of AB. Arsenic species formation was influenced by culture regime, with PO4-riboside produced under nutrient rich conditions, whereas Dimethylarsenoacetate (DMAA) was found in old (.42 days old) batch cultures, with this arsenic species possibly produced by the degradation of arsenoribosides-arsenolipids from decomposing cells rather than by biosynthesis. Nutrient availability, hence culture regime was thus influential in directly and indirectly influencing arsenic cycling and the arsenic species produced by D. tertiolecta and T. pseudonana. Future research should thus utilise continuous culture regimes to study arsenic cycling as these are far more analogous to environmental processes.

    AB - Arsenic cycling by the marine phytoplankton Dunaliella tertiolecta and the marine diatom Thalassiosira pseudonana was influenced by culture regime. Arsenic was associated with the residue cell fractions of batch cultured phytoplankton (D. tertiolecta and T. pseudonana), due to the accumulation of dead cells within batch cultures. Greater arsenic concentrations were associated with water-soluble and lipid- oluble cell fractions of continuously cultured phytoplankton. Arsenoribosides (as glycerol (Gly-), phosphate (PO4-) and sulfate (OSO3-)) were ubiquitous in D. tertiolecta (Gly- and PO4- only) and T. pseudonana (all three species). Additionally, arsenobetaine (AB) was not detected in any phytoplankton tissues, illustrating that marine phytoplankton themselves are not an alternate source of AB. Arsenic species formation was influenced by culture regime, with PO4-riboside produced under nutrient rich conditions, whereas Dimethylarsenoacetate (DMAA) was found in old (.42 days old) batch cultures, with this arsenic species possibly produced by the degradation of arsenoribosides-arsenolipids from decomposing cells rather than by biosynthesis. Nutrient availability, hence culture regime was thus influential in directly and indirectly influencing arsenic cycling and the arsenic species produced by D. tertiolecta and T. pseudonana. Future research should thus utilise continuous culture regimes to study arsenic cycling as these are far more analogous to environmental processes.

    KW - arsenic species

    KW - arsenoribosides

    KW - batch culture

    KW - continuous culture

    KW - lipid-soluble arsenic.

    U2 - 10.1071/EN12191

    DO - 10.1071/EN12191

    M3 - Article

    VL - 10

    SP - 91

    EP - 101

    JO - Environmental Chemistry

    JF - Environmental Chemistry

    SN - 1448-2517

    ER -