Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism

Y Foong, D Jans, Michael Rolph, Michelle Gahan, Suresh Mahalingam

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Interleukin-15 (IL-15) is a potent growth factor for activated T and natural killer (NK) cells, stimulator of memory T cells and plays an important role in viral immunity. To investigate mechanisms underlying the antiviral activity of IL-15, a recombinant vaccinia virus (rVV) encoding murine IL-15 (VV-IL-15) was constructed. Following infection of mice with VV-IL-15, virus titres in the ovaries were significantly reduced compared to mice infected with control VV. Growth of VV-IL-15 was also reduced in nude athymic mice, indicating the antiviral activity of IL-15 does not require T cells. Additionally, VV-IL-15 augmented the cytolytic activity of natural NK cells in the spleen and enhanced interferon (IFN) mRNA expression and transcription factors associated with IFN induction. Using knockout mice and antibody depletion studies, we showed for the first time that the control of VV-IL-15 replication in mice is dependent on NK cells and IFNs and, in their absence, the protective role of IL-15 is abolished.
    Original languageEnglish
    Pages (from-to)228-237
    Number of pages10
    JournalVirology
    Volume393
    Issue number2
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Interleukin-15
    Antiviral Agents
    Nude Mice
    Natural Killer Cells
    Interferons
    T-Lymphocytes
    Natural Killer T-Cells
    Vaccinia virus
    Viral Load
    Knockout Mice
    Ovary
    Immunity
    Intercellular Signaling Peptides and Proteins
    Transcription Factors
    Spleen
    Messenger RNA
    Antibodies

    Cite this

    Foong, Y ; Jans, D ; Rolph, Michael ; Gahan, Michelle ; Mahalingam, Suresh. / Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism. In: Virology. 2009 ; Vol. 393, No. 2. pp. 228-237.
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    abstract = "Interleukin-15 (IL-15) is a potent growth factor for activated T and natural killer (NK) cells, stimulator of memory T cells and plays an important role in viral immunity. To investigate mechanisms underlying the antiviral activity of IL-15, a recombinant vaccinia virus (rVV) encoding murine IL-15 (VV-IL-15) was constructed. Following infection of mice with VV-IL-15, virus titres in the ovaries were significantly reduced compared to mice infected with control VV. Growth of VV-IL-15 was also reduced in nude athymic mice, indicating the antiviral activity of IL-15 does not require T cells. Additionally, VV-IL-15 augmented the cytolytic activity of natural NK cells in the spleen and enhanced interferon (IFN) mRNA expression and transcription factors associated with IFN induction. Using knockout mice and antibody depletion studies, we showed for the first time that the control of VV-IL-15 replication in mice is dependent on NK cells and IFNs and, in their absence, the protective role of IL-15 is abolished.",
    author = "Y Foong and D Jans and Michael Rolph and Michelle Gahan and Suresh Mahalingam",
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    Foong, Y, Jans, D, Rolph, M, Gahan, M & Mahalingam, S 2009, 'Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism', Virology, vol. 393, no. 2, pp. 228-237. https://doi.org/10.1016/j.virol.2009.07.030

    Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism. / Foong, Y; Jans, D; Rolph, Michael; Gahan, Michelle; Mahalingam, Suresh.

    In: Virology, Vol. 393, No. 2, 2009, p. 228-237.

    Research output: Contribution to journalArticle

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