Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism

Y Foong, D Jans, Michael Rolph, Michelle Gahan, Suresh Mahalingam

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Interleukin-15 (IL-15) is a potent growth factor for activated T and natural killer (NK) cells, stimulator of memory T cells and plays an important role in viral immunity. To investigate mechanisms underlying the antiviral activity of IL-15, a recombinant vaccinia virus (rVV) encoding murine IL-15 (VV-IL-15) was constructed. Following infection of mice with VV-IL-15, virus titres in the ovaries were significantly reduced compared to mice infected with control VV. Growth of VV-IL-15 was also reduced in nude athymic mice, indicating the antiviral activity of IL-15 does not require T cells. Additionally, VV-IL-15 augmented the cytolytic activity of natural NK cells in the spleen and enhanced interferon (IFN) mRNA expression and transcription factors associated with IFN induction. Using knockout mice and antibody depletion studies, we showed for the first time that the control of VV-IL-15 replication in mice is dependent on NK cells and IFNs and, in their absence, the protective role of IL-15 is abolished.
Original languageEnglish
Pages (from-to)228-237
Number of pages10
JournalVirology
Volume393
Issue number2
DOIs
Publication statusPublished - 2009

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Interleukin-15
Antiviral Agents
Nude Mice
Natural Killer Cells
Interferons
T-Lymphocytes
Natural Killer T-Cells
Vaccinia virus
Viral Load
Knockout Mice
Ovary
Immunity
Intercellular Signaling Peptides and Proteins
Transcription Factors
Spleen
Messenger RNA
Antibodies

Cite this

Foong, Y ; Jans, D ; Rolph, Michael ; Gahan, Michelle ; Mahalingam, Suresh. / Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism. In: Virology. 2009 ; Vol. 393, No. 2. pp. 228-237.
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title = "Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism",
abstract = "Interleukin-15 (IL-15) is a potent growth factor for activated T and natural killer (NK) cells, stimulator of memory T cells and plays an important role in viral immunity. To investigate mechanisms underlying the antiviral activity of IL-15, a recombinant vaccinia virus (rVV) encoding murine IL-15 (VV-IL-15) was constructed. Following infection of mice with VV-IL-15, virus titres in the ovaries were significantly reduced compared to mice infected with control VV. Growth of VV-IL-15 was also reduced in nude athymic mice, indicating the antiviral activity of IL-15 does not require T cells. Additionally, VV-IL-15 augmented the cytolytic activity of natural NK cells in the spleen and enhanced interferon (IFN) mRNA expression and transcription factors associated with IFN induction. Using knockout mice and antibody depletion studies, we showed for the first time that the control of VV-IL-15 replication in mice is dependent on NK cells and IFNs and, in their absence, the protective role of IL-15 is abolished.",
author = "Y Foong and D Jans and Michael Rolph and Michelle Gahan and Suresh Mahalingam",
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Foong, Y, Jans, D, Rolph, M, Gahan, M & Mahalingam, S 2009, 'Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism', Virology, vol. 393, no. 2, pp. 228-237. https://doi.org/10.1016/j.virol.2009.07.030

Interleukin-15 mediates potent antiviral responses via an interferoj-dependent mechanism. / Foong, Y; Jans, D; Rolph, Michael; Gahan, Michelle; Mahalingam, Suresh.

In: Virology, Vol. 393, No. 2, 2009, p. 228-237.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Jans, D

AU - Rolph, Michael

AU - Gahan, Michelle

AU - Mahalingam, Suresh

PY - 2009

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AB - Interleukin-15 (IL-15) is a potent growth factor for activated T and natural killer (NK) cells, stimulator of memory T cells and plays an important role in viral immunity. To investigate mechanisms underlying the antiviral activity of IL-15, a recombinant vaccinia virus (rVV) encoding murine IL-15 (VV-IL-15) was constructed. Following infection of mice with VV-IL-15, virus titres in the ovaries were significantly reduced compared to mice infected with control VV. Growth of VV-IL-15 was also reduced in nude athymic mice, indicating the antiviral activity of IL-15 does not require T cells. Additionally, VV-IL-15 augmented the cytolytic activity of natural NK cells in the spleen and enhanced interferon (IFN) mRNA expression and transcription factors associated with IFN induction. Using knockout mice and antibody depletion studies, we showed for the first time that the control of VV-IL-15 replication in mice is dependent on NK cells and IFNs and, in their absence, the protective role of IL-15 is abolished.

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