In this study, a survey was conducted to determine the prevalence of intestinal parasites among pupils of UNICEF primary school in Isingwu Oraifite, Ekwusigo Local Government Area, Anambra State, Southeastern Nigeria. Of the 462 surveyed pupils, 220 (47.6%) had parasitic infection. Seven intestinal parasites were isolated, namely, (12.8%), Hookworm (7.6%), (4.8%), (1.9%), (11.0%), (6.7%), (2.8%). Prevalence rate was higher among males (p > 0.5) than females though not statistically significance. Pupils in the age-group10-14 years had the highest prevalence value (61.4%) while those in age-group 5-9 years had the lowest prevalence value (24.1%). Infection was higher among those whose parents were farmers and traders than those of civil servants. Prevalence was also higher among nursery school pupils. Health education, sanitation and mass chemotherapy should be encouraged in order to control intestinal parasitic disease in the school.