Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone

A. Plant, E. A. McLaughlin, W. C.L. Ford

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective: To determine if human spermatozoa could be immobilized and intracellular calcium measurements made on individual cells to measure what proportion can respond to P. Design: Spermatozoa were loaded with Fura 2 (Sigma Chemical Co., Poole, Dorset, United Kingdom) and suspended in 10% gelatin at 37°C. A thin layer of the suspension was cooled to room temperature (20°C to 25°C) and [Ca2+](i) was measured with a fluorescence microscope equipped with dual wavelength excitation and an image analysis system. Setting: University-based laboratory. Participants: Semen was obtained from four fertile donors to a donor insemination program. Interventions: None. Main Outcome Measures: [Ca2+](i) was calculated from the ratio of Fura 2 fluorescence excited at 340 nm and that excited at 366 nm. Results: One hundred six of 114 sperm examined (93%) demonstrated a significant response to P but the size and duration of the response was variable. Conclusion: These data demonstrate that most sperm can respond to P.

Original languageEnglish
Pages (from-to)1213-1215
Number of pages3
JournalFertility and Sterility
Volume64
Issue number6
DOIs
Publication statusPublished - Dec 1995
Externally publishedYes

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Progesterone
Spermatozoa
Calcium
Fura-2
Fluorescence
Insemination
Gelatin
Semen
Suspensions
Outcome Assessment (Health Care)
Temperature

Cite this

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title = "Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone",
abstract = "Objective: To determine if human spermatozoa could be immobilized and intracellular calcium measurements made on individual cells to measure what proportion can respond to P. Design: Spermatozoa were loaded with Fura 2 (Sigma Chemical Co., Poole, Dorset, United Kingdom) and suspended in 10{\%} gelatin at 37°C. A thin layer of the suspension was cooled to room temperature (20°C to 25°C) and [Ca2+](i) was measured with a fluorescence microscope equipped with dual wavelength excitation and an image analysis system. Setting: University-based laboratory. Participants: Semen was obtained from four fertile donors to a donor insemination program. Interventions: None. Main Outcome Measures: [Ca2+](i) was calculated from the ratio of Fura 2 fluorescence excited at 340 nm and that excited at 366 nm. Results: One hundred six of 114 sperm examined (93{\%}) demonstrated a significant response to P but the size and duration of the response was variable. Conclusion: These data demonstrate that most sperm can respond to P.",
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Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone. / Plant, A.; McLaughlin, E. A.; Ford, W. C.L.

In: Fertility and Sterility, Vol. 64, No. 6, 12.1995, p. 1213-1215.

Research output: Contribution to journalArticle

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AU - Plant, A.

AU - McLaughlin, E. A.

AU - Ford, W. C.L.

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