Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

C.H. Frère, P.J. Prentis, T. Ezaz, A. Georges

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Habitat fragmentation as a result of urbanisation
is a growing problem for native lizard species. The
Eastern Water Dragon (Physignathus lesueurii) is a social
arboreal agamid lizard, native to Australia. This species
represents an ideal model species to investigate the effect
of urbanisation because of their prominent abundance in
the urban landscape. Here we describe the isolation and
characterisation of a novel set of 74 di-, tri-, and tetramicrosatellites
from which 18 were selected and optimised
into two multiplexes. The 18 microsatellites generated a
total 148 alleles across the two populations. The number of
alleles per locus varied from 2 to 18 alleles and measures
of Ho and He varied from 0.395 to 0.877 and from 0.441
to 0.880, respectively. We also present primers for four
novel mitochondrial DNA (mtDNA) markers. The combined
length of the four mtDNA marker pairs was 2,528 bp
which included 15 nucleotides changes. In comparison to
threatened species, which are generally characterised by
small population sizes, the Eastern Water Dragon represents
an ideal model species to investigate the effect of
urbanisation on their behavioural ecology and connectivity
patterns among populations.
Original languageUndefined
Pages (from-to)113-116
Number of pages4
JournalConservation Genetics Resources
Volume4
Issue number1
DOIs
Publication statusPublished - 2012

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