Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

C.H. Frère, P.J. Prentis, T. Ezaz, A. Georges

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    Habitat fragmentation as a result of urbanisation
    is a growing problem for native lizard species. The
    Eastern Water Dragon (Physignathus lesueurii) is a social
    arboreal agamid lizard, native to Australia. This species
    represents an ideal model species to investigate the effect
    of urbanisation because of their prominent abundance in
    the urban landscape. Here we describe the isolation and
    characterisation of a novel set of 74 di-, tri-, and tetramicrosatellites
    from which 18 were selected and optimised
    into two multiplexes. The 18 microsatellites generated a
    total 148 alleles across the two populations. The number of
    alleles per locus varied from 2 to 18 alleles and measures
    of Ho and He varied from 0.395 to 0.877 and from 0.441
    to 0.880, respectively. We also present primers for four
    novel mitochondrial DNA (mtDNA) markers. The combined
    length of the four mtDNA marker pairs was 2,528 bp
    which included 15 nucleotides changes. In comparison to
    threatened species, which are generally characterised by
    small population sizes, the Eastern Water Dragon represents
    an ideal model species to investigate the effect of
    urbanisation on their behavioural ecology and connectivity
    patterns among populations.
    Original languageUndefined
    Pages (from-to)113-116
    Number of pages4
    JournalConservation Genetics Resources
    Issue number1
    Publication statusPublished - 2012

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