Isolation and physical mapping of sex-linked AFLP markers in the Nile tilapia (Oreochromis niloticus L.)

Tariq Ezaz, Simon Harvey, Chuta Boomphakdee, Alan Teale, Brendan McAndrew, David Penman

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Gynogenetically produced XX and YY Nile tilapia (Oreochromis niloticus) and diploid control groups were screened for amplified fragment length polymorphisms (AFLPs) to search for sex-linked or sex-specific markers. Family-level bulked segregant analysis (XX and YY gynogenetic family pools) and individual screening (XX and YY gynogenetics and XX and XY control individuals) identified 3 Y-linked (OniY425, OniY382, OniY227) and one X-linked (OniX420) AFLP markers. OniX420 and OniY425 were shown to be allelic. Single locus polymerase chain reaction assays were developed for these markers. Tight linkage was demonstrated between the AFLP markers and the sex locus within the source families. However, these markers failed to consistently identify sex in unrelated individuals, indicating recombination between the markers and the sex-determining loci. O. niloticus bacterial artificial chromosome clones, containing the AFLP markers, hybridized to the long arm of chromosome 1. This confirmed previous evidence, based on meiotic chromosome pairing and fluorescence in situ hybridization probes obtained through chromosome microdissection, that chromosome pair 1 is the sex chromosomes.
Original languageEnglish
Pages (from-to)435-445
Number of pages11
JournalMarine Biotechnology
Volume6
Issue number5
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Cichlids
physical chromosome mapping
Oreochromis niloticus
amplified fragment length polymorphism
polymorphism
chromosome
gender
Chromosomes, Human, Pair 1
chromosomes
loci
Chromosome Pairing
Bacterial Artificial Chromosomes
Microdissection
Sex Chromosomes
chromosome pairing
bacterial artificial chromosomes
sex chromosomes
fluorescence in situ hybridization
Diploidy
Fluorescence In Situ Hybridization

Cite this

Ezaz, Tariq ; Harvey, Simon ; Boomphakdee, Chuta ; Teale, Alan ; McAndrew, Brendan ; Penman, David. / Isolation and physical mapping of sex-linked AFLP markers in the Nile tilapia (Oreochromis niloticus L.). In: Marine Biotechnology. 2004 ; Vol. 6, No. 5. pp. 435-445.
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Isolation and physical mapping of sex-linked AFLP markers in the Nile tilapia (Oreochromis niloticus L.). / Ezaz, Tariq; Harvey, Simon; Boomphakdee, Chuta; Teale, Alan; McAndrew, Brendan; Penman, David.

In: Marine Biotechnology, Vol. 6, No. 5, 2004, p. 435-445.

Research output: Contribution to journalArticle

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