Isolation and purification of orientin and vitexin from Trollius chinensis Bunge by high-speed counter-current chromatography

Xu HUANG, Dat TRAN

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Orientin and vitexin are the two main bioactive compounds in Trollius chinensis Bunge. In this study, a rapid method was established for the isolation and purification of orientin and vitexin from T. chinensis Bunge using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-ethanol-water (4:1:5, v/v/v). A total of 9.8 mg orientin and 2.1 mg vitexin were obtained from 100 mg of the ethyl acetate extract, with purities of 99.2% and 96.0%, respectively. Their structures were identified by UV, MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of orientin and vitexin from T. chinensis Bunge.

    Original languageEnglish
    Pages (from-to)674-676
    Number of pages3
    JournalInternational Journal of Emerging Technology and Advanced Engineering
    Volume28
    Issue number9
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Trollius chinensis
    countercurrent chromatography
    Chromatography
    Purification
    rapid methods
    purity
    ethanol
    Ethanol
    extracts
    Nuclear magnetic resonance
    orientin
    vitexin
    Water
    water

    Cite this

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    abstract = "Orientin and vitexin are the two main bioactive compounds in Trollius chinensis Bunge. In this study, a rapid method was established for the isolation and purification of orientin and vitexin from T. chinensis Bunge using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-ethanol-water (4:1:5, v/v/v). A total of 9.8 mg orientin and 2.1 mg vitexin were obtained from 100 mg of the ethyl acetate extract, with purities of 99.2{\%} and 96.0{\%}, respectively. Their structures were identified by UV, MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of orientin and vitexin from T. chinensis Bunge.",
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    TY - JOUR

    T1 - Isolation and purification of orientin and vitexin from Trollius chinensis Bunge by high-speed counter-current chromatography

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    AU - TRAN, Dat

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