Karyological Characterization of the Butterfly Lizard (Leiolepis reevesii rubritaeniata, Agamidae, Squamata) by Molecular Cytogenetic Approach

Kornsorn Srikulnath, Kazumi Matsubara, Yoshinobu Uno, Amara Thongpan, Saowanee Suputtitada, Somsak Apisitwanich, Yoichi Matsuda, Chizuko Nishida

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L4m + L2sm + M2m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p.
Original languageEnglish
Pages (from-to)213-223
Number of pages11
JournalCytogenetic and Genome Research
Volume125
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Butterflies
Lizards
rRNA Genes
Cytogenetics
Sex Chromosomes
Chromosomes
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 6
Snakes
Constitution and Bylaws
Karyotype
Constriction
Chickens
Staining and Labeling
Genes

Cite this

Srikulnath, Kornsorn ; Matsubara, Kazumi ; Uno, Yoshinobu ; Thongpan, Amara ; Suputtitada, Saowanee ; Apisitwanich, Somsak ; Matsuda, Yoichi ; Nishida, Chizuko. / Karyological Characterization of the Butterfly Lizard (Leiolepis reevesii rubritaeniata, Agamidae, Squamata) by Molecular Cytogenetic Approach. In: Cytogenetic and Genome Research. 2009 ; Vol. 125. pp. 213-223.
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abstract = "Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L4m + L2sm + M2m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p.",
keywords = "Butterfly lizard, CGH, FISH, Gene mapping, Microchromosome, rRNA genes, Sex chromosome, Tandem fusion, Telomere.",
author = "Kornsorn Srikulnath and Kazumi Matsubara and Yoshinobu Uno and Amara Thongpan and Saowanee Suputtitada and Somsak Apisitwanich and Yoichi Matsuda and Chizuko Nishida",
year = "2009",
doi = "10.1159/000230005",
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Srikulnath, K, Matsubara, K, Uno, Y, Thongpan, A, Suputtitada, S, Apisitwanich, S, Matsuda, Y & Nishida, C 2009, 'Karyological Characterization of the Butterfly Lizard (Leiolepis reevesii rubritaeniata, Agamidae, Squamata) by Molecular Cytogenetic Approach', Cytogenetic and Genome Research, vol. 125, pp. 213-223. https://doi.org/10.1159/000230005

Karyological Characterization of the Butterfly Lizard (Leiolepis reevesii rubritaeniata, Agamidae, Squamata) by Molecular Cytogenetic Approach. / Srikulnath, Kornsorn; Matsubara, Kazumi; Uno, Yoshinobu; Thongpan, Amara; Suputtitada, Saowanee; Apisitwanich, Somsak; Matsuda, Yoichi; Nishida, Chizuko.

In: Cytogenetic and Genome Research, Vol. 125, 2009, p. 213-223.

Research output: Contribution to journalArticle

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T1 - Karyological Characterization of the Butterfly Lizard (Leiolepis reevesii rubritaeniata, Agamidae, Squamata) by Molecular Cytogenetic Approach

AU - Srikulnath, Kornsorn

AU - Matsubara, Kazumi

AU - Uno, Yoshinobu

AU - Thongpan, Amara

AU - Suputtitada, Saowanee

AU - Apisitwanich, Somsak

AU - Matsuda, Yoichi

AU - Nishida, Chizuko

PY - 2009

Y1 - 2009

N2 - Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L4m + L2sm + M2m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p.

AB - Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L4m + L2sm + M2m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p.

KW - Butterfly lizard

KW - CGH

KW - FISH

KW - Gene mapping

KW - Microchromosome

KW - rRNA genes

KW - Sex chromosome

KW - Tandem fusion

KW - Telomere.

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DO - 10.1159/000230005

M3 - Article

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SP - 213

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JO - Cytogenetics and Cell Genetics

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