TY - JOUR
T1 - Karyotype characterisation of two Australian dragon lizards (Squamata
T2 - Agamidae: Amphibolurinae) reveals subtle chromosomal rearrangements between related species with similar karyotypes
AU - Alam, Shayer M.I.
AU - Sarre, Stephen D.
AU - Georges, Arthur
AU - Ezaz, Tariq
N1 - Funding Information:
The work was supported in part by a grant from the Australian Research Council (DP170101147) awarded to A.G., T.E., and S.D.S. T. lineata animals provided for this work as part of Australian Research Council grant (LP110200029) awarded to S.D.S., T.E., and A.G. S.M.I.A. is supported by the International Research Training Program-Higher Degree by Research (RTP-HDR) Scholarship of the University of Canberra.
Publisher Copyright:
© 2020 S. Karger AG, Basel. Copyright: All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Agamid lizards (Squamata: Agamidae) are karyotypically heterogeneous. Among the 101 species currently described from Australia, all are from the subfamily Amphibolurinae. This group is, with some exceptions, karyotypically conserved, and all species involving heterogametic sex show female heterogamety. Here, we describe the chromosomes of 2 additional Australian agamid lizards, Tympanocryptis lineata and Rankinia diemensis. These species are phylogenetically and cytogenetically sisters to the well-characterised Pogona vitticeps, but their sex chromosomes and other chromosomal characteristics are unknown. In this study, we applied advanced molecular cytogenetic techniques, such as fluorescence in situ hybridisation (FISH) and cross-species gene mapping, to characterise chromosomes and to identify sex chromosomes in these species. Our data suggest that both species have a conserved karyotype with P. vitticeps but with subtle rearrangements in the chromosomal landscapes. We could identify that T. lineata possesses a female heterogametic system (ZZ/ZW) with a pair of sex microchromosomes, while R. diemensis may have heterogametic sex chromosomes, but this requires further investigations. Our study shows the pattern of chromosomal rearrangements between closely related species, explaining the speciation within Australian agamid lizards of similar karyotypes.
AB - Agamid lizards (Squamata: Agamidae) are karyotypically heterogeneous. Among the 101 species currently described from Australia, all are from the subfamily Amphibolurinae. This group is, with some exceptions, karyotypically conserved, and all species involving heterogametic sex show female heterogamety. Here, we describe the chromosomes of 2 additional Australian agamid lizards, Tympanocryptis lineata and Rankinia diemensis. These species are phylogenetically and cytogenetically sisters to the well-characterised Pogona vitticeps, but their sex chromosomes and other chromosomal characteristics are unknown. In this study, we applied advanced molecular cytogenetic techniques, such as fluorescence in situ hybridisation (FISH) and cross-species gene mapping, to characterise chromosomes and to identify sex chromosomes in these species. Our data suggest that both species have a conserved karyotype with P. vitticeps but with subtle rearrangements in the chromosomal landscapes. We could identify that T. lineata possesses a female heterogametic system (ZZ/ZW) with a pair of sex microchromosomes, while R. diemensis may have heterogametic sex chromosomes, but this requires further investigations. Our study shows the pattern of chromosomal rearrangements between closely related species, explaining the speciation within Australian agamid lizards of similar karyotypes.
KW - Animals
KW - Chromosome Banding
KW - Chromosome Mapping
KW - Chromosomes, Artificial, Bacterial/genetics
KW - Chromosomes/genetics
KW - Clone Cells
KW - DNA Methylation/genetics
KW - Gene Rearrangement/genetics
KW - Geography
KW - Karyotype
KW - Lizards/genetics
KW - Microsatellite Repeats/genetics
KW - Species Specificity
KW - Telomere/genetics
UR - http://www.scopus.com/inward/record.url?scp=85096616122&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/5d84f782-ee64-326d-bd44-a2aee95cc500/
U2 - 10.1159/000511344
DO - 10.1159/000511344
M3 - Article
C2 - 33207346
AN - SCOPUS:85096616122
VL - 160
SP - 610
EP - 624
JO - Cytogenetics and Cell Genetics
JF - Cytogenetics and Cell Genetics
SN - 0301-0171
IS - 10
ER -