TY - JOUR
T1 - Karyotypes and Sex Chromosomes in Two Australian Native Freshwater Fishes, Golden Perch (Macquaria ambigua) and Murray Cod (Maccullochella peelii) (Percichthyidae)
AU - Shams, Foyez
AU - Dyer, Fiona
AU - Thompson, Ross
AU - Duncan, Richard P.
AU - Thiem, Jason D.
AU - Majtánová, Zuzana
AU - Ezaz, Tariq
PY - 2019/8/30
Y1 - 2019/8/30
N2 - Karyotypic data from Australian native freshwater fishes are scarce, having been described from relatively few species. Golden perch (Macquaria ambigua) and Murray cod (Maccullochella peelii) are two large-bodied freshwater fish species native to Australia with significant indigenous, cultural, recreational and commercial value. The arid landscape over much of these fishes' range, coupled with the boom and bust hydrology of their habitat, means that these species have potential to provide useful evolutionary insights, such as karyotypes and sex chromosome evolution in vertebrates. Here we applied standard and molecular cytogenetic techniques to characterise karyotypes for golden perch and Murray cod. Both species have a diploid chromosome number 2n = 48 and a male heterogametic sex chromosome system (XX/XY). While the karyotype of golden perch is composed exclusively of acrocentric chromosomes, the karyotype of Murray cod consists of two submetacentric and 46 subtelocentric/acrocentric chromosomes. We have identified variable accumulation of repetitive sequences (AAT)10 and (CGG)10 along with diverse methylation patterns, especially on the sex chromosomes in both species. Our study provides a baseline for future cytogenetic analyses of other Australian freshwater fishes, especially species from the family Percichthyidae, to better understand their genome and sex chromosome evolution.
AB - Karyotypic data from Australian native freshwater fishes are scarce, having been described from relatively few species. Golden perch (Macquaria ambigua) and Murray cod (Maccullochella peelii) are two large-bodied freshwater fish species native to Australia with significant indigenous, cultural, recreational and commercial value. The arid landscape over much of these fishes' range, coupled with the boom and bust hydrology of their habitat, means that these species have potential to provide useful evolutionary insights, such as karyotypes and sex chromosome evolution in vertebrates. Here we applied standard and molecular cytogenetic techniques to characterise karyotypes for golden perch and Murray cod. Both species have a diploid chromosome number 2n = 48 and a male heterogametic sex chromosome system (XX/XY). While the karyotype of golden perch is composed exclusively of acrocentric chromosomes, the karyotype of Murray cod consists of two submetacentric and 46 subtelocentric/acrocentric chromosomes. We have identified variable accumulation of repetitive sequences (AAT)10 and (CGG)10 along with diverse methylation patterns, especially on the sex chromosomes in both species. Our study provides a baseline for future cytogenetic analyses of other Australian freshwater fishes, especially species from the family Percichthyidae, to better understand their genome and sex chromosome evolution.
KW - comparative genomic hybridisation (CGH)
KW - DNA methylation
KW - florescence in situ hybridisation (FISH)
KW - karyogram
KW - sex determination
KW - Karyogram
KW - Sex determination
KW - Comparative genomic hybridisation (CGH)
KW - Florescence in situ hybridisation (FISH)
UR - http://www.scopus.com/inward/record.url?scp=85071781994&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/karyotypes-sex-chromosomes-two-australian-native-freshwater-fishes-golden-perch-macquaria-ambigua-mu
U2 - 10.3390/ijms20174244
DO - 10.3390/ijms20174244
M3 - Article
C2 - 31480228
AN - SCOPUS:85071781994
SN - 1661-6596
VL - 20
SP - 1
EP - 14
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 17
M1 - 4244
ER -