Kif4 is essential for mouse oocyte meiosis

Nicole J. Camlin, Eileen A. McLaughlin, Janet E. Holt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Progression through the meiotic cell cycle must be strictly regulated in oocytes to generate viable embryos and offspring. During mitosis, the kinesin motor protein Kif4 is indispensable for chromosome condensation and separation, midzone formation and cytokinesis. Additionally, the bioactivity of Kif4 is dependent on phosphorylation via Aurora Kinase B and Cdk1, which regulate Kif4 function throughout mitosis. Here, we examine the role of Kif4 in mammalian oocyte meiosis. Kif4 localized in the cytoplasm throughout meiosis I and II, but was also observed to have a dynamic subcellular distribution, associating with both microtubules and kinetochores at different stages of development. Co-localization and proximity ligation assays revealed that the kinetochore proteins, CENP-C and Ndc80, are potential Kif4 interacting proteins. Functional analysis of Kif4 in oocytes via antisense knock-down demonstrated that this protein was not essential for meiosis I completion. However, Kif4 depleted oocytes displayed enlarged polar bodies and abnormal metaphase II spindles, indicating an essential role for this protein for correct asymmetric cell division in meiosis I. Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENPC. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age.

Original languageEnglish
Article numbere0170650
Pages (from-to)1-10
Number of pages10
JournalPLoS One
Volume12
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

Fingerprint

Meiosis
meiosis
Oocytes
oocytes
Kinetochores
mice
kinetochores
Proteins
proteins
Mitosis
mitosis
Aurora Kinase B
Aurora Kinases
Asymmetric Cell Division
phosphotransferases (kinases)
Polar Bodies
Kinesin
Cells
Cytokinesis
kinesin

Cite this

Camlin, N. J., McLaughlin, E. A., & Holt, J. E. (2017). Kif4 is essential for mouse oocyte meiosis. PLoS One, 12(1), 1-10. [e0170650]. https://doi.org/10.1371/journal.pone.0170650
Camlin, Nicole J. ; McLaughlin, Eileen A. ; Holt, Janet E. / Kif4 is essential for mouse oocyte meiosis. In: PLoS One. 2017 ; Vol. 12, No. 1. pp. 1-10.
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Camlin, NJ, McLaughlin, EA & Holt, JE 2017, 'Kif4 is essential for mouse oocyte meiosis', PLoS One, vol. 12, no. 1, e0170650, pp. 1-10. https://doi.org/10.1371/journal.pone.0170650

Kif4 is essential for mouse oocyte meiosis. / Camlin, Nicole J.; McLaughlin, Eileen A.; Holt, Janet E.

In: PLoS One, Vol. 12, No. 1, e0170650, 01.01.2017, p. 1-10.

Research output: Contribution to journalArticle

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KW - Cell Cycle Proteins/genetics

KW - Mice

KW - Mitosis/genetics

KW - Kinesin/genetics

KW - CDC2 Protein Kinase/genetics

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Camlin NJ, McLaughlin EA, Holt JE. Kif4 is essential for mouse oocyte meiosis. PLoS One. 2017 Jan 1;12(1):1-10. e0170650. https://doi.org/10.1371/journal.pone.0170650