TY - JOUR
T1 - Longitudinal Survey of Fecal Microbiota in Healthy Dogs Administered a Commercial Probiotic
AU - Ciaravolo, Susan
AU - Martínez-López, Lina María
AU - Allcock, Richard J.N.
AU - Woodward, Andrew P.
AU - Mansfield, Caroline
N1 - Funding Information:
The authors acknowledge the support of Leilani Santos during the experiments. Funding. The authors received funding for this work from Blackmores Limited.
Publisher Copyright:
© Copyright © 2021 Ciaravolo, Martínez-López, Allcock, Woodward and Mansfield.
PY - 2021/6/21
Y1 - 2021/6/21
N2 - The aim of this longitudinal microbiome study was to investigate the effects of a commercially available veterinary synbiotic product (Blackmore's® Paw DigestiCare 60™) on the fecal microbiome of healthy dogs using 16S rRNA gene microbial profiling. Fifteen healthy, privately-owned dogs participated in a 2-week trial administration of the product. Fecal samples were collected at different time points, including baseline (prior to treatment), during administration and after discontinuation of product. Large intra- and inter-individual variation was observed throughout the study, but microbiome composition at higher phylogenetic levels, alpha and beta diversity were not significantly altered after 2 weeks of probiotic administration, suggesting an absence of probiotic impact on microbial diversity. Administration of the synbiotic preparation did, however, result in transient increases in probiotic species from Enterococacceae and Streptococacceae families as well as an increase in Fusobacteria; with the fecal microbiota partially reverting to its baseline state 3-weeks after cessation of probiotic administration.
AB - The aim of this longitudinal microbiome study was to investigate the effects of a commercially available veterinary synbiotic product (Blackmore's® Paw DigestiCare 60™) on the fecal microbiome of healthy dogs using 16S rRNA gene microbial profiling. Fifteen healthy, privately-owned dogs participated in a 2-week trial administration of the product. Fecal samples were collected at different time points, including baseline (prior to treatment), during administration and after discontinuation of product. Large intra- and inter-individual variation was observed throughout the study, but microbiome composition at higher phylogenetic levels, alpha and beta diversity were not significantly altered after 2 weeks of probiotic administration, suggesting an absence of probiotic impact on microbial diversity. Administration of the synbiotic preparation did, however, result in transient increases in probiotic species from Enterococacceae and Streptococacceae families as well as an increase in Fusobacteria; with the fecal microbiota partially reverting to its baseline state 3-weeks after cessation of probiotic administration.
KW - Bifidobacterium
KW - Enterococcus
KW - Lactobacillus
KW - microbiota
KW - probiotic
KW - Streptococcus
KW - synbiotic
UR - http://www.scopus.com/inward/record.url?scp=85109067216&partnerID=8YFLogxK
U2 - 10.3389/fvets.2021.664318
DO - 10.3389/fvets.2021.664318
M3 - Article
AN - SCOPUS:85109067216
SN - 2297-1769
VL - 8
SP - 1
EP - 12
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
M1 - 664318
ER -