Measure of stress response induced by temperature and salinity changes on hatched

Elisabeth Deschaseaux, Anne Taylor, William Maher

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    To better understand the cascade of molecular reactions leading to delayed development and mortality of early life stages of marine intertidal gastropods, in response to temperature and salinity changes associated with climate change, three biomarkers: total antioxidant capacity, lipid peroxidation and lysosomal stability were investigated on hatched larvae. Encapsulated embryos of three marine gastropod species (Bembicium nanum, Siphonaria denticulata and Dolabrifera brazieri), which have already proven responsive to thermal and osmotic variations, were exposed to six combinations of temperature (22 °C and 30 °C) and salinity (25⿰, 35⿰and 45⿰) until the larvae hatched. Time to hatching was affected by salinity and temperature in all three species. High salinity (45⿰) generally retarded the hatching process although the response was speciesspecific for temperature. Total antioxidant capacity and lipid peroxidation were also highly species-specific with the general trend showing that these biomarkers were adversely affected by high temperature (30 °C) at salinities of 25⿰ and 45⿰. Bembicium nanum lysosomal destabilisation increased significantly with an increase in temperature and salinity (30 °C and 45⿰) and this was associated with delayed development and increased mortality. Investigations on the additional biomarker, lysosomal stability, gave a clearer picture of the numerous and complex molecular and cellular mechanisms leading to mortality and underdevelopment in response to environmental stress for this species. As few differences were observed in the enzymatic biomarkers total antioxidant capacity and lipid peroxidation between hatched larvae and the previously investigated encapsulated embryo response to thermal and osmotic stress, it is suggested that further studies could be undertaken using embryos encapsulated in egg masses, as it is less time consuming than working on hatched larvae.
    Original languageEnglish
    Pages (from-to)121-128
    Number of pages8
    JournalJournal of Experimental Marine Biology and Ecology
    Volume397
    DOIs
    Publication statusPublished - 2011

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    induced response
    stress response
    salinity
    antioxidant
    larva
    biomarker
    embryo
    lipid
    biomarkers
    embryo (animal)
    temperature
    lipid peroxidation
    mortality
    larvae
    gastropod
    hatching
    antioxidants
    Gastropoda
    environmental stress
    thermal stress

    Cite this

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    abstract = "To better understand the cascade of molecular reactions leading to delayed development and mortality of early life stages of marine intertidal gastropods, in response to temperature and salinity changes associated with climate change, three biomarkers: total antioxidant capacity, lipid peroxidation and lysosomal stability were investigated on hatched larvae. Encapsulated embryos of three marine gastropod species (Bembicium nanum, Siphonaria denticulata and Dolabrifera brazieri), which have already proven responsive to thermal and osmotic variations, were exposed to six combinations of temperature (22 {\^A}°C and 30 {\^A}°C) and salinity (25{\^a}¿°, 35{\^a}¿°and 45{\^a}¿°) until the larvae hatched. Time to hatching was affected by salinity and temperature in all three species. High salinity (45{\^a}¿°) generally retarded the hatching process although the response was speciesspecific for temperature. Total antioxidant capacity and lipid peroxidation were also highly species-specific with the general trend showing that these biomarkers were adversely affected by high temperature (30 {\^A}°C) at salinities of 25{\^a}¿° and 45{\^a}¿°. Bembicium nanum lysosomal destabilisation increased significantly with an increase in temperature and salinity (30 {\^A}°C and 45{\^a}¿°) and this was associated with delayed development and increased mortality. Investigations on the additional biomarker, lysosomal stability, gave a clearer picture of the numerous and complex molecular and cellular mechanisms leading to mortality and underdevelopment in response to environmental stress for this species. As few differences were observed in the enzymatic biomarkers total antioxidant capacity and lipid peroxidation between hatched larvae and the previously investigated encapsulated embryo response to thermal and osmotic stress, it is suggested that further studies could be undertaken using embryos encapsulated in egg masses, as it is less time consuming than working on hatched larvae.",
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    language = "English",
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    pages = "121--128",
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    Measure of stress response induced by temperature and salinity changes on hatched. / Deschaseaux, Elisabeth ; Taylor, Anne; Maher, William.

    In: Journal of Experimental Marine Biology and Ecology, Vol. 397, 2011, p. 121-128.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Measure of stress response induced by temperature and salinity changes on hatched

    AU - Deschaseaux, Elisabeth

    AU - Taylor, Anne

    AU - Maher, William

    PY - 2011

    Y1 - 2011

    N2 - To better understand the cascade of molecular reactions leading to delayed development and mortality of early life stages of marine intertidal gastropods, in response to temperature and salinity changes associated with climate change, three biomarkers: total antioxidant capacity, lipid peroxidation and lysosomal stability were investigated on hatched larvae. Encapsulated embryos of three marine gastropod species (Bembicium nanum, Siphonaria denticulata and Dolabrifera brazieri), which have already proven responsive to thermal and osmotic variations, were exposed to six combinations of temperature (22 °C and 30 °C) and salinity (25⿰, 35⿰and 45⿰) until the larvae hatched. Time to hatching was affected by salinity and temperature in all three species. High salinity (45⿰) generally retarded the hatching process although the response was speciesspecific for temperature. Total antioxidant capacity and lipid peroxidation were also highly species-specific with the general trend showing that these biomarkers were adversely affected by high temperature (30 °C) at salinities of 25⿰ and 45⿰. Bembicium nanum lysosomal destabilisation increased significantly with an increase in temperature and salinity (30 °C and 45⿰) and this was associated with delayed development and increased mortality. Investigations on the additional biomarker, lysosomal stability, gave a clearer picture of the numerous and complex molecular and cellular mechanisms leading to mortality and underdevelopment in response to environmental stress for this species. As few differences were observed in the enzymatic biomarkers total antioxidant capacity and lipid peroxidation between hatched larvae and the previously investigated encapsulated embryo response to thermal and osmotic stress, it is suggested that further studies could be undertaken using embryos encapsulated in egg masses, as it is less time consuming than working on hatched larvae.

    AB - To better understand the cascade of molecular reactions leading to delayed development and mortality of early life stages of marine intertidal gastropods, in response to temperature and salinity changes associated with climate change, three biomarkers: total antioxidant capacity, lipid peroxidation and lysosomal stability were investigated on hatched larvae. Encapsulated embryos of three marine gastropod species (Bembicium nanum, Siphonaria denticulata and Dolabrifera brazieri), which have already proven responsive to thermal and osmotic variations, were exposed to six combinations of temperature (22 °C and 30 °C) and salinity (25⿰, 35⿰and 45⿰) until the larvae hatched. Time to hatching was affected by salinity and temperature in all three species. High salinity (45⿰) generally retarded the hatching process although the response was speciesspecific for temperature. Total antioxidant capacity and lipid peroxidation were also highly species-specific with the general trend showing that these biomarkers were adversely affected by high temperature (30 °C) at salinities of 25⿰ and 45⿰. Bembicium nanum lysosomal destabilisation increased significantly with an increase in temperature and salinity (30 °C and 45⿰) and this was associated with delayed development and increased mortality. Investigations on the additional biomarker, lysosomal stability, gave a clearer picture of the numerous and complex molecular and cellular mechanisms leading to mortality and underdevelopment in response to environmental stress for this species. As few differences were observed in the enzymatic biomarkers total antioxidant capacity and lipid peroxidation between hatched larvae and the previously investigated encapsulated embryo response to thermal and osmotic stress, it is suggested that further studies could be undertaken using embryos encapsulated in egg masses, as it is less time consuming than working on hatched larvae.

    KW - Antioxidant

    KW - Hatching

    KW - Larvae

    KW - Lipid peroxidation

    KW - Lysosomal stability

    U2 - 10.1016/j.jembe.2010.11.023

    DO - 10.1016/j.jembe.2010.11.023

    M3 - Article

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    SP - 121

    EP - 128

    JO - Journal of Experimental Marine Biology and Ecology

    JF - Journal of Experimental Marine Biology and Ecology

    SN - 0022-0981

    ER -