Measurement of arsenic species in marine macroalgae by microwave-assisted extraction and high performance liquid chromatography-inductively coupled plasma mass spectrometry

Rehema Tukai, William Maher, Ian McNaught, Michael Ellwood

    Research output: Contribution to journalArticle

    75 Citations (Scopus)

    Abstract

    The measurement of arsenic species (arsenoribosides, arsenate, dimethyl arsenic and monomethyl arsenic) in marine macroalgae by microwave-assisted extraction and HPLC–ICP-MS is described. The extraction of arsenic from three different macroalgae classes was optimised using a chemometric approach, with solvent composition and sample mass being the two significant factors influencing the extraction of arsenic. Extraction temperature and extraction time did not significantly influence the extraction of arsenic from macroalgae. The optimised conditions for arsenic extraction (methanol (%)) were: 56% for phaeophyta, 66% for rhodophyta and 78% for chlorophyta, (sample mass in 10 ml of solvent) 0.05 g for phaeophyta, 0.07 g for rhodophyta and 0.08 g for chlorophyta. When two extractions were used, the percentage of arsenic extracted from macroalgae was greater than 88%. Unambiguous separation and identification of three arsenoribosides (phosphate-, sulfonate- and sulfate-arsenoriboside) was achieved by chromatographing extracts on a Hamilton PRP X-100 anion exchange column with ammonium phosphate buffer as the mobile phase at a pH of 9.2. The unambiguous separation and identification of the glycerol-arsenoriboside was achieved by chromatographing extracts on a Supelcosil SCX cation exchange column with a pyridine–formic acid buffer as the mobile phase at a pH of 2.6
    Original languageEnglish
    Pages (from-to)173-185
    Number of pages13
    JournalAnalytica Chimica Acta
    Volume457
    Issue number2
    Publication statusPublished - 2002

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    Seaweed
    Inductively coupled plasma mass spectrometry
    High performance liquid chromatography
    Arsenic
    Microwaves
    liquid chromatography
    arsenic
    Mass Spectrometry
    mass spectrometry
    High Pressure Liquid Chromatography
    plasma
    Phaeophyta
    Rhodophyta
    Chlorophyta
    ion exchange
    Buffers
    phosphate
    microwave
    sulfonate
    arsenate

    Cite this

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    title = "Measurement of arsenic species in marine macroalgae by microwave-assisted extraction and high performance liquid chromatography-inductively coupled plasma mass spectrometry",
    abstract = "The measurement of arsenic species (arsenoribosides, arsenate, dimethyl arsenic and monomethyl arsenic) in marine macroalgae by microwave-assisted extraction and HPLC–ICP-MS is described. The extraction of arsenic from three different macroalgae classes was optimised using a chemometric approach, with solvent composition and sample mass being the two significant factors influencing the extraction of arsenic. Extraction temperature and extraction time did not significantly influence the extraction of arsenic from macroalgae. The optimised conditions for arsenic extraction (methanol ({\%})) were: 56{\%} for phaeophyta, 66{\%} for rhodophyta and 78{\%} for chlorophyta, (sample mass in 10 ml of solvent) 0.05 g for phaeophyta, 0.07 g for rhodophyta and 0.08 g for chlorophyta. When two extractions were used, the percentage of arsenic extracted from macroalgae was greater than 88{\%}. Unambiguous separation and identification of three arsenoribosides (phosphate-, sulfonate- and sulfate-arsenoriboside) was achieved by chromatographing extracts on a Hamilton PRP X-100 anion exchange column with ammonium phosphate buffer as the mobile phase at a pH of 9.2. The unambiguous separation and identification of the glycerol-arsenoriboside was achieved by chromatographing extracts on a Supelcosil SCX cation exchange column with a pyridine–formic acid buffer as the mobile phase at a pH of 2.6",
    author = "Rehema Tukai and William Maher and Ian McNaught and Michael Ellwood",
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    Measurement of arsenic species in marine macroalgae by microwave-assisted extraction and high performance liquid chromatography-inductively coupled plasma mass spectrometry. / Tukai, Rehema; Maher, William; McNaught, Ian; Ellwood, Michael.

    In: Analytica Chimica Acta, Vol. 457, No. 2, 2002, p. 173-185.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Measurement of arsenic species in marine macroalgae by microwave-assisted extraction and high performance liquid chromatography-inductively coupled plasma mass spectrometry

    AU - Tukai, Rehema

    AU - Maher, William

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    AU - Ellwood, Michael

    PY - 2002

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    AB - The measurement of arsenic species (arsenoribosides, arsenate, dimethyl arsenic and monomethyl arsenic) in marine macroalgae by microwave-assisted extraction and HPLC–ICP-MS is described. The extraction of arsenic from three different macroalgae classes was optimised using a chemometric approach, with solvent composition and sample mass being the two significant factors influencing the extraction of arsenic. Extraction temperature and extraction time did not significantly influence the extraction of arsenic from macroalgae. The optimised conditions for arsenic extraction (methanol (%)) were: 56% for phaeophyta, 66% for rhodophyta and 78% for chlorophyta, (sample mass in 10 ml of solvent) 0.05 g for phaeophyta, 0.07 g for rhodophyta and 0.08 g for chlorophyta. When two extractions were used, the percentage of arsenic extracted from macroalgae was greater than 88%. Unambiguous separation and identification of three arsenoribosides (phosphate-, sulfonate- and sulfate-arsenoriboside) was achieved by chromatographing extracts on a Hamilton PRP X-100 anion exchange column with ammonium phosphate buffer as the mobile phase at a pH of 9.2. The unambiguous separation and identification of the glycerol-arsenoriboside was achieved by chromatographing extracts on a Supelcosil SCX cation exchange column with a pyridine–formic acid buffer as the mobile phase at a pH of 2.6

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