Measurement of selenomethionine and selenocysteine in fish tissues using HPLC-ICP-MS

Rajani Jagtap, Bill MAHER, Simon FOSTER, Michael Ellwood

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The extraction and measurement of selenomethionine (SeMet) and selenocysteine (SeCys) in fish muscle and liver tissues were evaluated. Preliminary experiments were undertaken with marine certified reference materials. NRCC TORT 1/2 Lobster hepatopancreas, NRCC DOLT 2/3; Dogfish liver and NIST 1566 a/b Oyster tissue were extracted using aqueous leaching, enzymatic hydrolysis and acid hydrolysis with ultrasound, microwave assisted heating and conventional heating using a hybridisation oven. High Se recoveries were obtained with the use of methanesulfonic acid as an extractant (89-92%); however, the low pH required for extraction is not compatible with most HPLC columns and dilution is required to analyse extracts that precluded the measurement of Se in samples from relatively uncontaminated environments. As well, Se species (SeMet and SeCys) degrade with the use of highly acidic extractants. Enzymatic hydrolysis with protease type XIV gave good recoveries (63-67%), but only SeMet was stable in the extract. As SeCys was not stable, a derivatisation step using iodoacetamide to form a stable carboxymethylated complex was used prior to enzymatic hydrolysis. Conventional heating at 37 °C for 20 h and microwave assisted heating at 45°C for 45 min gave similar recoveries for CRMs but conventional heating gave slightly better recoveries for fish tissues.Two separate complementary methods were developed for the measurement of SeMet and SeCys in marine fish tissues; SeMet was measured after protease XIV hydrolysis and analysis using anion or cation exchange chromatography-ICPMS, while for the measurement of SeCys a carbamidomethylation derivatisation procedure prior to the protease hydrolysis was used and the separation of the species was carried out using size exclusion chromatography, followed by C8 ion-pair reversed-phase chromatography with ICPMS. Recoveries of SeMet and SeCys added to CRMs and fish tissues were 97 ± 9% and 84 ± 9% respectively.
    Original languageEnglish
    Pages (from-to)248-257
    Number of pages10
    JournalMicrochemical Journal
    Volume128
    DOIs
    Publication statusPublished - 2016

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    Selenocysteine
    Selenomethionine
    Fish
    Tissue
    Enzymatic hydrolysis
    Heating
    Recovery
    Hydrolysis
    Pronase
    Chromatography
    Liver
    Microwaves
    Iodoacetamide
    Size exclusion chromatography
    Ovens
    Leaching
    Dilution
    Anions
    Muscle
    Cations

    Cite this

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    title = "Measurement of selenomethionine and selenocysteine in fish tissues using HPLC-ICP-MS",
    abstract = "The extraction and measurement of selenomethionine (SeMet) and selenocysteine (SeCys) in fish muscle and liver tissues were evaluated. Preliminary experiments were undertaken with marine certified reference materials. NRCC TORT 1/2 Lobster hepatopancreas, NRCC DOLT 2/3; Dogfish liver and NIST 1566 a/b Oyster tissue were extracted using aqueous leaching, enzymatic hydrolysis and acid hydrolysis with ultrasound, microwave assisted heating and conventional heating using a hybridisation oven. High Se recoveries were obtained with the use of methanesulfonic acid as an extractant (89-92{\%}); however, the low pH required for extraction is not compatible with most HPLC columns and dilution is required to analyse extracts that precluded the measurement of Se in samples from relatively uncontaminated environments. As well, Se species (SeMet and SeCys) degrade with the use of highly acidic extractants. Enzymatic hydrolysis with protease type XIV gave good recoveries (63-67{\%}), but only SeMet was stable in the extract. As SeCys was not stable, a derivatisation step using iodoacetamide to form a stable carboxymethylated complex was used prior to enzymatic hydrolysis. Conventional heating at 37 °C for 20 h and microwave assisted heating at 45°C for 45 min gave similar recoveries for CRMs but conventional heating gave slightly better recoveries for fish tissues.Two separate complementary methods were developed for the measurement of SeMet and SeCys in marine fish tissues; SeMet was measured after protease XIV hydrolysis and analysis using anion or cation exchange chromatography-ICPMS, while for the measurement of SeCys a carbamidomethylation derivatisation procedure prior to the protease hydrolysis was used and the separation of the species was carried out using size exclusion chromatography, followed by C8 ion-pair reversed-phase chromatography with ICPMS. Recoveries of SeMet and SeCys added to CRMs and fish tissues were 97 ± 9{\%} and 84 ± 9{\%} respectively.",
    author = "Rajani Jagtap and Bill MAHER and Simon FOSTER and Michael Ellwood",
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    language = "English",
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    Measurement of selenomethionine and selenocysteine in fish tissues using HPLC-ICP-MS. / Jagtap, Rajani; MAHER, Bill; FOSTER, Simon; Ellwood, Michael.

    In: Microchemical Journal, Vol. 128, 2016, p. 248-257.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Measurement of selenomethionine and selenocysteine in fish tissues using HPLC-ICP-MS

    AU - Jagtap, Rajani

    AU - MAHER, Bill

    AU - FOSTER, Simon

    AU - Ellwood, Michael

    PY - 2016

    Y1 - 2016

    N2 - The extraction and measurement of selenomethionine (SeMet) and selenocysteine (SeCys) in fish muscle and liver tissues were evaluated. Preliminary experiments were undertaken with marine certified reference materials. NRCC TORT 1/2 Lobster hepatopancreas, NRCC DOLT 2/3; Dogfish liver and NIST 1566 a/b Oyster tissue were extracted using aqueous leaching, enzymatic hydrolysis and acid hydrolysis with ultrasound, microwave assisted heating and conventional heating using a hybridisation oven. High Se recoveries were obtained with the use of methanesulfonic acid as an extractant (89-92%); however, the low pH required for extraction is not compatible with most HPLC columns and dilution is required to analyse extracts that precluded the measurement of Se in samples from relatively uncontaminated environments. As well, Se species (SeMet and SeCys) degrade with the use of highly acidic extractants. Enzymatic hydrolysis with protease type XIV gave good recoveries (63-67%), but only SeMet was stable in the extract. As SeCys was not stable, a derivatisation step using iodoacetamide to form a stable carboxymethylated complex was used prior to enzymatic hydrolysis. Conventional heating at 37 °C for 20 h and microwave assisted heating at 45°C for 45 min gave similar recoveries for CRMs but conventional heating gave slightly better recoveries for fish tissues.Two separate complementary methods were developed for the measurement of SeMet and SeCys in marine fish tissues; SeMet was measured after protease XIV hydrolysis and analysis using anion or cation exchange chromatography-ICPMS, while for the measurement of SeCys a carbamidomethylation derivatisation procedure prior to the protease hydrolysis was used and the separation of the species was carried out using size exclusion chromatography, followed by C8 ion-pair reversed-phase chromatography with ICPMS. Recoveries of SeMet and SeCys added to CRMs and fish tissues were 97 ± 9% and 84 ± 9% respectively.

    AB - The extraction and measurement of selenomethionine (SeMet) and selenocysteine (SeCys) in fish muscle and liver tissues were evaluated. Preliminary experiments were undertaken with marine certified reference materials. NRCC TORT 1/2 Lobster hepatopancreas, NRCC DOLT 2/3; Dogfish liver and NIST 1566 a/b Oyster tissue were extracted using aqueous leaching, enzymatic hydrolysis and acid hydrolysis with ultrasound, microwave assisted heating and conventional heating using a hybridisation oven. High Se recoveries were obtained with the use of methanesulfonic acid as an extractant (89-92%); however, the low pH required for extraction is not compatible with most HPLC columns and dilution is required to analyse extracts that precluded the measurement of Se in samples from relatively uncontaminated environments. As well, Se species (SeMet and SeCys) degrade with the use of highly acidic extractants. Enzymatic hydrolysis with protease type XIV gave good recoveries (63-67%), but only SeMet was stable in the extract. As SeCys was not stable, a derivatisation step using iodoacetamide to form a stable carboxymethylated complex was used prior to enzymatic hydrolysis. Conventional heating at 37 °C for 20 h and microwave assisted heating at 45°C for 45 min gave similar recoveries for CRMs but conventional heating gave slightly better recoveries for fish tissues.Two separate complementary methods were developed for the measurement of SeMet and SeCys in marine fish tissues; SeMet was measured after protease XIV hydrolysis and analysis using anion or cation exchange chromatography-ICPMS, while for the measurement of SeCys a carbamidomethylation derivatisation procedure prior to the protease hydrolysis was used and the separation of the species was carried out using size exclusion chromatography, followed by C8 ion-pair reversed-phase chromatography with ICPMS. Recoveries of SeMet and SeCys added to CRMs and fish tissues were 97 ± 9% and 84 ± 9% respectively.

    U2 - 10.1016/j.microc.2016.04.021

    DO - 10.1016/j.microc.2016.04.021

    M3 - Article

    VL - 128

    SP - 248

    EP - 257

    JO - Microchemical Journal

    JF - Microchemical Journal

    SN - 0026-265X

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