Measurement of water-soluble arsenic species in freeze-dried marine animal tissues by microwave-assisted extraction and HPLC-ICP-MS

J. Kirby, William Maher

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    Abstract

    A microwave-assisted procedure is outlined for the extraction of water-soluble arsenic in freeze-dried marine animal tissues. The optimum microwave-assisted conditions were three extractions with 50% (v/v) methanol–water at 70 to 75 °C for 5 min. Quantitative extraction of arsenic in the water-soluble fraction of dogfish muscle (Dorm-2: 103 ± 2%) is consistent with that reported in the literature for this tissue. Lower extraction efficiencies for arsenic were found for liver (e.g. Dolt-1: 75 ± 5%; Pomatomus saltatrix: 80%), digestive (e.g. Tort-2: 92 ± 5%; Mugil cephalus stomach: 58%) and whole (e.g. Mussel CRM 278R: 66.1 ± 0.5%; Bembicium auratum: 76.4%) tissues. Arsenic extraction efficiencies in the water-soluble fraction were slightly higher for Dogfish Liver (Dolt-1) and Oyster (SRM 1566a) compared to that reported in the literature for these tissues. These results indicate that when samples are prepared in a similar manner, the efficiency to extract arsenic in the methanol–water soluble fraction will depend on the marine animal species and tissue analysed. The robustness of the microwave-assisted extraction procedure to identify and quantify arsenic species in freeze-dried marine animal tissues was determined using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and the certified reference materials Dogfish Muscle (Dorm-2) and Lobster Hepatopancreas (Tort-2). Arsenic species determined in Dorm-2 tissue were AsB (16.80 ± 0.14 µg g−1), TMAP (0.17 ± 0.01 µg g−1), AsC (0.023 ± 0.002 µg g−1), TETRA (0.24 ± 0.02 µg g−1) and DMA (0.280 ± 0.004 µg g−1). Arsenic species determined in Tort-2 tissue were AsB (13.10 ± 0.08 µg g−1), TMAP (1.20 ± 0.03 µg g−1), AsC (trace), TETRA (0.055 ± 0.005 µg g−1), DMA (1.03 ± 0.10 µg g−1), MA (0.20 ± 0.01 µg g−1), As+5 (0.41 ± 0.03 µg g−1) and phosphate arsenoribose (0.13 ± 0.03 µg g−1). Two unknown anionic and one cationic arsenic species were also identified in Tort-2 tissue.
    Original languageEnglish
    Pages (from-to)838-843
    Number of pages6
    JournalJournal of Analytical Atomic Spectrometry
    Volume17
    Issue number8
    Publication statusPublished - 2002

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    Arsenic
    Animals
    Microwaves
    Tissue
    Water
    Dynamic mechanical analysis
    Liver
    Muscle
    Inductively coupled plasma mass spectrometry
    High performance liquid chromatography
    Phosphates

    Cite this

    @article{7b02a3b1567d41d0be38cea1c2ad2216,
    title = "Measurement of water-soluble arsenic species in freeze-dried marine animal tissues by microwave-assisted extraction and HPLC-ICP-MS",
    abstract = "A microwave-assisted procedure is outlined for the extraction of water-soluble arsenic in freeze-dried marine animal tissues. The optimum microwave-assisted conditions were three extractions with 50{\%} (v/v) methanol–water at 70 to 75 °C for 5 min. Quantitative extraction of arsenic in the water-soluble fraction of dogfish muscle (Dorm-2: 103 ± 2{\%}) is consistent with that reported in the literature for this tissue. Lower extraction efficiencies for arsenic were found for liver (e.g. Dolt-1: 75 ± 5{\%}; Pomatomus saltatrix: 80{\%}), digestive (e.g. Tort-2: 92 ± 5{\%}; Mugil cephalus stomach: 58{\%}) and whole (e.g. Mussel CRM 278R: 66.1 ± 0.5{\%}; Bembicium auratum: 76.4{\%}) tissues. Arsenic extraction efficiencies in the water-soluble fraction were slightly higher for Dogfish Liver (Dolt-1) and Oyster (SRM 1566a) compared to that reported in the literature for these tissues. These results indicate that when samples are prepared in a similar manner, the efficiency to extract arsenic in the methanol–water soluble fraction will depend on the marine animal species and tissue analysed. The robustness of the microwave-assisted extraction procedure to identify and quantify arsenic species in freeze-dried marine animal tissues was determined using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and the certified reference materials Dogfish Muscle (Dorm-2) and Lobster Hepatopancreas (Tort-2). Arsenic species determined in Dorm-2 tissue were AsB (16.80 ± 0.14 µg g−1), TMAP (0.17 ± 0.01 µg g−1), AsC (0.023 ± 0.002 µg g−1), TETRA (0.24 ± 0.02 µg g−1) and DMA (0.280 ± 0.004 µg g−1). Arsenic species determined in Tort-2 tissue were AsB (13.10 ± 0.08 µg g−1), TMAP (1.20 ± 0.03 µg g−1), AsC (trace), TETRA (0.055 ± 0.005 µg g−1), DMA (1.03 ± 0.10 µg g−1), MA (0.20 ± 0.01 µg g−1), As+5 (0.41 ± 0.03 µg g−1) and phosphate arsenoribose (0.13 ± 0.03 µg g−1). Two unknown anionic and one cationic arsenic species were also identified in Tort-2 tissue.",
    author = "J. Kirby and William Maher",
    year = "2002",
    language = "English",
    volume = "17",
    pages = "838--843",
    journal = "Annual Reports on Analytical Atomic Spectroscopy",
    issn = "0267-9477",
    publisher = "Royal Society of Chemistry",
    number = "8",

    }

    TY - JOUR

    T1 - Measurement of water-soluble arsenic species in freeze-dried marine animal tissues by microwave-assisted extraction and HPLC-ICP-MS

    AU - Kirby, J.

    AU - Maher, William

    PY - 2002

    Y1 - 2002

    N2 - A microwave-assisted procedure is outlined for the extraction of water-soluble arsenic in freeze-dried marine animal tissues. The optimum microwave-assisted conditions were three extractions with 50% (v/v) methanol–water at 70 to 75 °C for 5 min. Quantitative extraction of arsenic in the water-soluble fraction of dogfish muscle (Dorm-2: 103 ± 2%) is consistent with that reported in the literature for this tissue. Lower extraction efficiencies for arsenic were found for liver (e.g. Dolt-1: 75 ± 5%; Pomatomus saltatrix: 80%), digestive (e.g. Tort-2: 92 ± 5%; Mugil cephalus stomach: 58%) and whole (e.g. Mussel CRM 278R: 66.1 ± 0.5%; Bembicium auratum: 76.4%) tissues. Arsenic extraction efficiencies in the water-soluble fraction were slightly higher for Dogfish Liver (Dolt-1) and Oyster (SRM 1566a) compared to that reported in the literature for these tissues. These results indicate that when samples are prepared in a similar manner, the efficiency to extract arsenic in the methanol–water soluble fraction will depend on the marine animal species and tissue analysed. The robustness of the microwave-assisted extraction procedure to identify and quantify arsenic species in freeze-dried marine animal tissues was determined using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and the certified reference materials Dogfish Muscle (Dorm-2) and Lobster Hepatopancreas (Tort-2). Arsenic species determined in Dorm-2 tissue were AsB (16.80 ± 0.14 µg g−1), TMAP (0.17 ± 0.01 µg g−1), AsC (0.023 ± 0.002 µg g−1), TETRA (0.24 ± 0.02 µg g−1) and DMA (0.280 ± 0.004 µg g−1). Arsenic species determined in Tort-2 tissue were AsB (13.10 ± 0.08 µg g−1), TMAP (1.20 ± 0.03 µg g−1), AsC (trace), TETRA (0.055 ± 0.005 µg g−1), DMA (1.03 ± 0.10 µg g−1), MA (0.20 ± 0.01 µg g−1), As+5 (0.41 ± 0.03 µg g−1) and phosphate arsenoribose (0.13 ± 0.03 µg g−1). Two unknown anionic and one cationic arsenic species were also identified in Tort-2 tissue.

    AB - A microwave-assisted procedure is outlined for the extraction of water-soluble arsenic in freeze-dried marine animal tissues. The optimum microwave-assisted conditions were three extractions with 50% (v/v) methanol–water at 70 to 75 °C for 5 min. Quantitative extraction of arsenic in the water-soluble fraction of dogfish muscle (Dorm-2: 103 ± 2%) is consistent with that reported in the literature for this tissue. Lower extraction efficiencies for arsenic were found for liver (e.g. Dolt-1: 75 ± 5%; Pomatomus saltatrix: 80%), digestive (e.g. Tort-2: 92 ± 5%; Mugil cephalus stomach: 58%) and whole (e.g. Mussel CRM 278R: 66.1 ± 0.5%; Bembicium auratum: 76.4%) tissues. Arsenic extraction efficiencies in the water-soluble fraction were slightly higher for Dogfish Liver (Dolt-1) and Oyster (SRM 1566a) compared to that reported in the literature for these tissues. These results indicate that when samples are prepared in a similar manner, the efficiency to extract arsenic in the methanol–water soluble fraction will depend on the marine animal species and tissue analysed. The robustness of the microwave-assisted extraction procedure to identify and quantify arsenic species in freeze-dried marine animal tissues was determined using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and the certified reference materials Dogfish Muscle (Dorm-2) and Lobster Hepatopancreas (Tort-2). Arsenic species determined in Dorm-2 tissue were AsB (16.80 ± 0.14 µg g−1), TMAP (0.17 ± 0.01 µg g−1), AsC (0.023 ± 0.002 µg g−1), TETRA (0.24 ± 0.02 µg g−1) and DMA (0.280 ± 0.004 µg g−1). Arsenic species determined in Tort-2 tissue were AsB (13.10 ± 0.08 µg g−1), TMAP (1.20 ± 0.03 µg g−1), AsC (trace), TETRA (0.055 ± 0.005 µg g−1), DMA (1.03 ± 0.10 µg g−1), MA (0.20 ± 0.01 µg g−1), As+5 (0.41 ± 0.03 µg g−1) and phosphate arsenoribose (0.13 ± 0.03 µg g−1). Two unknown anionic and one cationic arsenic species were also identified in Tort-2 tissue.

    M3 - Article

    VL - 17

    SP - 838

    EP - 843

    JO - Annual Reports on Analytical Atomic Spectroscopy

    JF - Annual Reports on Analytical Atomic Spectroscopy

    SN - 0267-9477

    IS - 8

    ER -