While research on work values has generated considerable scholarly attention, research on religiously oriented values, particularly on Islamic work values, has remained relatively limited in the extant literature. This paper attempts to address this lacuna by empirically measuring and validating the constructs of Islamic work value. The study adopted sequential exploratory mixed methods in which a quantitative research method was employed subsequent to qualitative methods in order to validate the psychometric properties of the Islamic work values' instrument. This study qualitatively validates 25 constructs of the 56 Islamic work values initially identified; of these, 13 constructs were quantitatively validated. Thus, this novel research contributes by establishing 13 Islamic work values in the literature, as well as developing an appropriate methodology for measuring and validating such Islamic work values. Finally, this paper identifies the limits of this study and suggests the potential areas of further research.