TY - CHAP
T1 - MicroRNA profiling from RSV-infected biofluids, whole blood, and tissue samples
AU - Anderson, Lydia
AU - Jorquera, Patricia A.
AU - Tripp, Ralph A.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016
Y1 - 2016
N2 - Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).
AB - Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).
KW - Exosomes
KW - MicroRNAs
KW - Respiratory syncytial virus
KW - RNA isolation
KW - RT-qPCR
UR - http://www.scopus.com/inward/record.url?scp=84981500000&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3687-8_14
DO - 10.1007/978-1-4939-3687-8_14
M3 - Chapter
C2 - 27464696
AN - SCOPUS:84981500000
SN - 9781493936854
VL - 1442
T3 - Methods in Molecular Biology
SP - 195
EP - 208
BT - Methods in Molecular Biology
A2 - Tripp, Ralph
A2 - Jorquera , Patricia
PB - Springer
CY - New York
ER -