MicroRNA profiling from RSV-infected biofluids, whole blood, and tissue samples

Lydia Anderson, Patricia A. Jorquera, Ralph A. Tripp

Research output: A Conference proceeding or a Chapter in BookChapterpeer-review

3 Citations (Scopus)


Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsRalph Tripp, Patricia Jorquera
Place of PublicationNew York
Number of pages14
ISBN (Electronic)9781493936878
ISBN (Print)9781493936854
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


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