Moderate exercise triggers both priming and activation of neutrophil subpopulations

John A. Smith, A. Bon Gray, David B. Pyne, Mark S. Baker, Richard D. Telford, Maurice J. Weidemann

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We investigated how moderate exercise affects neutrophil microbicidal activity and whether exercise-induced responses are associated with changes in growth hormone (GH) secretion. Biological fluctuations were controlled for and GH secretion was manipulated by glucose ingestion. In eight men, 1 h of moderate exercise increased intracellular H2O2 generation in response to phorbol 12-myristate 13-acetate stimulation by threefold (P = 0.025) and complement receptor expression by 20% (P = 0.045). These responses were accompanied by a twofold increase in the plasma concentration of elastase, a marker of neutrophil activation in vivo. The plasma concentration of GH increased 10-fold after exercise, but this was reduced to 3-fold by glucose ingestion (P < 0.001), which also blunted elastase release (P < 0.001). Although the magnitude of H2O2 generation increased in proportion to the increase in plasma GH concentration, it declined progressively once this exceeded 20 ng/ml. The net response of neutrophils to exercise may represent a balance between the individual responses of subpopulations that are unaffected, primed, or fully activated by circulating mediators that respond to exercise and to dietary glucose intake.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume270
Issue number4 39-4
Publication statusPublished - 13 May 1996

Fingerprint

Neutrophil Activation
Exercise
Growth Hormone
Pancreatic Elastase
Glucose
Neutrophils
Eating
Complement Receptors
Acetates

Cite this

Smith, John A. ; Gray, A. Bon ; Pyne, David B. ; Baker, Mark S. ; Telford, Richard D. ; Weidemann, Maurice J. / Moderate exercise triggers both priming and activation of neutrophil subpopulations. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1996 ; Vol. 270, No. 4 39-4.
@article{261f6c7102d34ec888590a3477782d47,
title = "Moderate exercise triggers both priming and activation of neutrophil subpopulations",
abstract = "We investigated how moderate exercise affects neutrophil microbicidal activity and whether exercise-induced responses are associated with changes in growth hormone (GH) secretion. Biological fluctuations were controlled for and GH secretion was manipulated by glucose ingestion. In eight men, 1 h of moderate exercise increased intracellular H2O2 generation in response to phorbol 12-myristate 13-acetate stimulation by threefold (P = 0.025) and complement receptor expression by 20{\%} (P = 0.045). These responses were accompanied by a twofold increase in the plasma concentration of elastase, a marker of neutrophil activation in vivo. The plasma concentration of GH increased 10-fold after exercise, but this was reduced to 3-fold by glucose ingestion (P < 0.001), which also blunted elastase release (P < 0.001). Although the magnitude of H2O2 generation increased in proportion to the increase in plasma GH concentration, it declined progressively once this exceeded 20 ng/ml. The net response of neutrophils to exercise may represent a balance between the individual responses of subpopulations that are unaffected, primed, or fully activated by circulating mediators that respond to exercise and to dietary glucose intake.",
keywords = "activation, flow cytometry, growth hormone, immunity",
author = "Smith, {John A.} and Gray, {A. Bon} and Pyne, {David B.} and Baker, {Mark S.} and Telford, {Richard D.} and Weidemann, {Maurice J.}",
year = "1996",
month = "5",
day = "13",
language = "English",
volume = "270",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "4 39-4",

}

Moderate exercise triggers both priming and activation of neutrophil subpopulations. / Smith, John A.; Gray, A. Bon; Pyne, David B.; Baker, Mark S.; Telford, Richard D.; Weidemann, Maurice J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 270, No. 4 39-4, 13.05.1996.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Moderate exercise triggers both priming and activation of neutrophil subpopulations

AU - Smith, John A.

AU - Gray, A. Bon

AU - Pyne, David B.

AU - Baker, Mark S.

AU - Telford, Richard D.

AU - Weidemann, Maurice J.

PY - 1996/5/13

Y1 - 1996/5/13

N2 - We investigated how moderate exercise affects neutrophil microbicidal activity and whether exercise-induced responses are associated with changes in growth hormone (GH) secretion. Biological fluctuations were controlled for and GH secretion was manipulated by glucose ingestion. In eight men, 1 h of moderate exercise increased intracellular H2O2 generation in response to phorbol 12-myristate 13-acetate stimulation by threefold (P = 0.025) and complement receptor expression by 20% (P = 0.045). These responses were accompanied by a twofold increase in the plasma concentration of elastase, a marker of neutrophil activation in vivo. The plasma concentration of GH increased 10-fold after exercise, but this was reduced to 3-fold by glucose ingestion (P < 0.001), which also blunted elastase release (P < 0.001). Although the magnitude of H2O2 generation increased in proportion to the increase in plasma GH concentration, it declined progressively once this exceeded 20 ng/ml. The net response of neutrophils to exercise may represent a balance between the individual responses of subpopulations that are unaffected, primed, or fully activated by circulating mediators that respond to exercise and to dietary glucose intake.

AB - We investigated how moderate exercise affects neutrophil microbicidal activity and whether exercise-induced responses are associated with changes in growth hormone (GH) secretion. Biological fluctuations were controlled for and GH secretion was manipulated by glucose ingestion. In eight men, 1 h of moderate exercise increased intracellular H2O2 generation in response to phorbol 12-myristate 13-acetate stimulation by threefold (P = 0.025) and complement receptor expression by 20% (P = 0.045). These responses were accompanied by a twofold increase in the plasma concentration of elastase, a marker of neutrophil activation in vivo. The plasma concentration of GH increased 10-fold after exercise, but this was reduced to 3-fold by glucose ingestion (P < 0.001), which also blunted elastase release (P < 0.001). Although the magnitude of H2O2 generation increased in proportion to the increase in plasma GH concentration, it declined progressively once this exceeded 20 ng/ml. The net response of neutrophils to exercise may represent a balance between the individual responses of subpopulations that are unaffected, primed, or fully activated by circulating mediators that respond to exercise and to dietary glucose intake.

KW - activation

KW - flow cytometry

KW - growth hormone

KW - immunity

UR - http://www.scopus.com/inward/record.url?scp=0030008122&partnerID=8YFLogxK

M3 - Article

VL - 270

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 4 39-4

ER -