Modulation of a surface antigen of Entamoeba histolytica in response to bacteria

A Bhattacharya; R Ghildyal; J Prasad; S Bhattacharya; L S Diamond

Modulation of a surface antigen of Entamoeba histolytica in response to bacteria

A Bhattacharya
, R Ghildyal
, J Prasad
, S Bhattacharya
, L S Diamond

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in the cell surface of Entamoeba histolytica, a human intestinal parasite and the causative agent of amebic dysentery, were examined with a monoclonal antibody, 2D7.10, which selectively recognizes carbohydrate epitopes in some axenic amebic strains. While high-level expression of this epitope was observed in axenic amebae, it was either absent or present only in small amounts in xenic amebae. Furthermore, reassociation of the axenic amebae with intestinal flora resulted in loss of the 2D7.10 epitope. Our data suggest that surface antigens of E. histolytica can be modulated in response to bacteria and may provide an explanation for the observed influence of bacteria on amebic virulence.

Original language	English
Pages (from-to)	1711-1713
Number of pages	3
Journal	Infection and Immunity
Volume	60
Issue number	4
Publication status	Published - Apr 1992
Externally published	Yes

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Cite this

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title = "Modulation of a surface antigen of Entamoeba histolytica in response to bacteria",

abstract = "Changes in the cell surface of Entamoeba histolytica, a human intestinal parasite and the causative agent of amebic dysentery, were examined with a monoclonal antibody, 2D7.10, which selectively recognizes carbohydrate epitopes in some axenic amebic strains. While high-level expression of this epitope was observed in axenic amebae, it was either absent or present only in small amounts in xenic amebae. Furthermore, reassociation of the axenic amebae with intestinal flora resulted in loss of the 2D7.10 epitope. Our data suggest that surface antigens of E. histolytica can be modulated in response to bacteria and may provide an explanation for the observed influence of bacteria on amebic virulence.",

keywords = "Animals, Antigens, Protozoan, Antigens, Surface, Bacteria, Blotting, Western, Entamoeba histolytica, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Bacterial, Symbiosis, Journal Article, Research Support, Non-U.S. Gov't",

author = "A Bhattacharya and R Ghildyal and J Prasad and S Bhattacharya and Diamond, \{L S\}",

year = "1992",

month = apr,

language = "English",

volume = "60",

pages = "1711--1713",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "4",

}

TY - JOUR

T1 - Modulation of a surface antigen of Entamoeba histolytica in response to bacteria

AU - Bhattacharya, A

AU - Ghildyal, R

AU - Prasad, J

AU - Bhattacharya, S

AU - Diamond, L S

PY - 1992/4

Y1 - 1992/4

N2 - Changes in the cell surface of Entamoeba histolytica, a human intestinal parasite and the causative agent of amebic dysentery, were examined with a monoclonal antibody, 2D7.10, which selectively recognizes carbohydrate epitopes in some axenic amebic strains. While high-level expression of this epitope was observed in axenic amebae, it was either absent or present only in small amounts in xenic amebae. Furthermore, reassociation of the axenic amebae with intestinal flora resulted in loss of the 2D7.10 epitope. Our data suggest that surface antigens of E. histolytica can be modulated in response to bacteria and may provide an explanation for the observed influence of bacteria on amebic virulence.

AB - Changes in the cell surface of Entamoeba histolytica, a human intestinal parasite and the causative agent of amebic dysentery, were examined with a monoclonal antibody, 2D7.10, which selectively recognizes carbohydrate epitopes in some axenic amebic strains. While high-level expression of this epitope was observed in axenic amebae, it was either absent or present only in small amounts in xenic amebae. Furthermore, reassociation of the axenic amebae with intestinal flora resulted in loss of the 2D7.10 epitope. Our data suggest that surface antigens of E. histolytica can be modulated in response to bacteria and may provide an explanation for the observed influence of bacteria on amebic virulence.

KW - Animals

KW - Antigens, Protozoan

KW - Antigens, Surface

KW - Bacteria

KW - Blotting, Western

KW - Entamoeba histolytica

KW - Enzyme-Linked Immunosorbent Assay

KW - Gene Expression Regulation, Bacterial

KW - Symbiosis

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 1548096

SN - 0019-9567

VL - 60

SP - 1711

EP - 1713

JO - Infection and Immunity

JF - Infection and Immunity

IS - 4

ER -

Modulation of a surface antigen of Entamoeba histolytica in response to bacteria

Abstract

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