Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia)

Kazumi Matsubara, Kazuhiko Yamada, Shu Umemoto, Kimiyuki Tsuchiya, Naoki Ikeda, Chizuko Nishida, Takahito Chijiwa, Kazuo Moriwaki, Yoichi Matsuda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. They were all localized to the centromeric regions of all autosomes and categorized into major satellite DNA, type I minor, and type II minor repetitive sequences. The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. The major satellite DNA sequence, which consisted of 30 bp elements, was organized in tandem arrays and constituted the majority of centromeric heterochromatin. Three families of repetitive sequences hybridized with B chromosomes in different patterns, suggesting that the B chromosomes of A. peninsulae were derived from A chromosomes and that the three repetitive sequences were amplified independently on each B chromosome. The minor repetitive sequences are present in the genomes of the other seven Apodemus species. In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. These results suggest that the major satellite DNA was amplified with base substitution in A. peninsulae after the divergence of the genus Apodemus from the common ancestor and that the B chromosomes of A. peninsulae might have a species-specific origin.
Original languageEnglish
Pages (from-to)1013-1026
Number of pages14
JournalChromosome Research
Volume16
DOIs
Publication statusPublished - 2008
Externally publishedYes

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Muridae
Chromosomes, Human, 4-5
Murinae
Heterochromatin
Nucleic Acid Repetitive Sequences
Molecular Cloning
Satellite DNA
Rodentia
Chromosomes, Human, 1-3
Chromosomes
Sequence Homology
In Situ Hybridization
Genome

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Matsubara, Kazumi ; Yamada, Kazuhiko ; Umemoto, Shu ; Tsuchiya, Kimiyuki ; Ikeda, Naoki ; Nishida, Chizuko ; Chijiwa, Takahito ; Moriwaki, Kazuo ; Matsuda, Yoichi. / Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia). In: Chromosome Research. 2008 ; Vol. 16. pp. 1013-1026.
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abstract = "Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. They were all localized to the centromeric regions of all autosomes and categorized into major satellite DNA, type I minor, and type II minor repetitive sequences. The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. The major satellite DNA sequence, which consisted of 30 bp elements, was organized in tandem arrays and constituted the majority of centromeric heterochromatin. Three families of repetitive sequences hybridized with B chromosomes in different patterns, suggesting that the B chromosomes of A. peninsulae were derived from A chromosomes and that the three repetitive sequences were amplified independently on each B chromosome. The minor repetitive sequences are present in the genomes of the other seven Apodemus species. In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. These results suggest that the major satellite DNA was amplified with base substitution in A. peninsulae after the divergence of the genus Apodemus from the common ancestor and that the B chromosomes of A. peninsulae might have a species-specific origin.",
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author = "Kazumi Matsubara and Kazuhiko Yamada and Shu Umemoto and Kimiyuki Tsuchiya and Naoki Ikeda and Chizuko Nishida and Takahito Chijiwa and Kazuo Moriwaki and Yoichi Matsuda",
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Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia). / Matsubara, Kazumi; Yamada, Kazuhiko; Umemoto, Shu; Tsuchiya, Kimiyuki; Ikeda, Naoki; Nishida, Chizuko; Chijiwa, Takahito; Moriwaki, Kazuo; Matsuda, Yoichi.

In: Chromosome Research, Vol. 16, 2008, p. 1013-1026.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia)

AU - Matsubara, Kazumi

AU - Yamada, Kazuhiko

AU - Umemoto, Shu

AU - Tsuchiya, Kimiyuki

AU - Ikeda, Naoki

AU - Nishida, Chizuko

AU - Chijiwa, Takahito

AU - Moriwaki, Kazuo

AU - Matsuda, Yoichi

PY - 2008

Y1 - 2008

N2 - Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. They were all localized to the centromeric regions of all autosomes and categorized into major satellite DNA, type I minor, and type II minor repetitive sequences. The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. The major satellite DNA sequence, which consisted of 30 bp elements, was organized in tandem arrays and constituted the majority of centromeric heterochromatin. Three families of repetitive sequences hybridized with B chromosomes in different patterns, suggesting that the B chromosomes of A. peninsulae were derived from A chromosomes and that the three repetitive sequences were amplified independently on each B chromosome. The minor repetitive sequences are present in the genomes of the other seven Apodemus species. In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. These results suggest that the major satellite DNA was amplified with base substitution in A. peninsulae after the divergence of the genus Apodemus from the common ancestor and that the B chromosomes of A. peninsulae might have a species-specific origin.

AB - Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. They were all localized to the centromeric regions of all autosomes and categorized into major satellite DNA, type I minor, and type II minor repetitive sequences. The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. The major satellite DNA sequence, which consisted of 30 bp elements, was organized in tandem arrays and constituted the majority of centromeric heterochromatin. Three families of repetitive sequences hybridized with B chromosomes in different patterns, suggesting that the B chromosomes of A. peninsulae were derived from A chromosomes and that the three repetitive sequences were amplified independently on each B chromosome. The minor repetitive sequences are present in the genomes of the other seven Apodemus species. In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. These results suggest that the major satellite DNA was amplified with base substitution in A. peninsulae after the divergence of the genus Apodemus from the common ancestor and that the B chromosomes of A. peninsulae might have a species-specific origin.

KW - Apodemus

KW - B chromosome

KW - centromere

KW - fluorescence in-situ hybridization

KW - heterochromatin

KW - repetitive sequence.

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DO - 10.1007/s10577-008-1259-x

M3 - Article

VL - 16

SP - 1013

EP - 1026

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

ER -