Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors

Yoshinao Katsu, Kazumi Matsubara, Satomi Kohno, Yoichi Matsuda, Michihisa Toriba, Kaori Oka, Louis Guillette, Yasuhiko Ohta, Taisen Iguchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In many vertebrates, steroid hormones are essential for ovarian differentiation during a critical developmental stage as well as promoting the growth and differentiation of the adult female reproductive system. Although studies have been extensively conducted in mammals and a few fish, amphibians, and bird species, the molecular mechanisms of sex steroid hormone (estrogens) action have been poorly examined in reptiles. Here, we evaluate hormone receptor and ligand interactions in two species of snake, the Okinawa habu (Protobothrops flavoviridis, Viperidae) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae) after the isolation of cDNAs encoding estrogen receptor A (ESR1) and estrogen receptor B(ESR2). Using a transient transfection assay with mammalian cells, the transcriptional activity of reptilian (Okinawa habu, Japanese four-striped rat snake, American alligator, and Florida red-belly freshwater turtle) ESR1 and ESR2 was examined. All ESR proteins displayed estrogen-dependent activation of transcription via an estrogen-response element-containing promoter; however, the responsiveness to various estrogens was different. Further, we determined the chromosomal locations of the snake steroid hormone receptor genes. ESR1 and ESR2 genes were localized to the short and long arms of chromosome 1, respectively, whereas androgen receptor was localized to a pair of microchromosomes in the two snake species examined. These data provide basic tools that allow future studies examining receptor-ligand interactions and steroid endocrinology in snakes and also expands our knowledge of sex steroid hormone receptor evolution.
Original languageEnglish
Pages (from-to)5710-5720
Number of pages11
JournalEndocrinology
Volume151
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Snakes
Chromosome Mapping
Molecular Cloning
Estrogen Receptors
Estrogens
Trimeresurus
Steroid Receptors
Gonadal Steroid Hormones
Hormones
Steroids
Colubridae
Ligands
Alligators and Crocodiles
Turtles
Reptiles
Endocrinology
Chromosomes, Human, Pair 1
Androgen Receptors
Response Elements
Amphibians

Cite this

Katsu, Y., Matsubara, K., Kohno, S., Matsuda, Y., Toriba, M., Oka, K., ... Iguchi, T. (2010). Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors. Endocrinology, 151, 5710-5720. https://doi.org/10.1210/en.2010-0356
Katsu, Yoshinao ; Matsubara, Kazumi ; Kohno, Satomi ; Matsuda, Yoichi ; Toriba, Michihisa ; Oka, Kaori ; Guillette, Louis ; Ohta, Yasuhiko ; Iguchi, Taisen. / Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors. In: Endocrinology. 2010 ; Vol. 151. pp. 5710-5720.
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abstract = "In many vertebrates, steroid hormones are essential for ovarian differentiation during a critical developmental stage as well as promoting the growth and differentiation of the adult female reproductive system. Although studies have been extensively conducted in mammals and a few fish, amphibians, and bird species, the molecular mechanisms of sex steroid hormone (estrogens) action have been poorly examined in reptiles. Here, we evaluate hormone receptor and ligand interactions in two species of snake, the Okinawa habu (Protobothrops flavoviridis, Viperidae) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae) after the isolation of cDNAs encoding estrogen receptor A (ESR1) and estrogen receptor B(ESR2). Using a transient transfection assay with mammalian cells, the transcriptional activity of reptilian (Okinawa habu, Japanese four-striped rat snake, American alligator, and Florida red-belly freshwater turtle) ESR1 and ESR2 was examined. All ESR proteins displayed estrogen-dependent activation of transcription via an estrogen-response element-containing promoter; however, the responsiveness to various estrogens was different. Further, we determined the chromosomal locations of the snake steroid hormone receptor genes. ESR1 and ESR2 genes were localized to the short and long arms of chromosome 1, respectively, whereas androgen receptor was localized to a pair of microchromosomes in the two snake species examined. These data provide basic tools that allow future studies examining receptor-ligand interactions and steroid endocrinology in snakes and also expands our knowledge of sex steroid hormone receptor evolution.",
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Katsu, Y, Matsubara, K, Kohno, S, Matsuda, Y, Toriba, M, Oka, K, Guillette, L, Ohta, Y & Iguchi, T 2010, 'Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors', Endocrinology, vol. 151, pp. 5710-5720. https://doi.org/10.1210/en.2010-0356

Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors. / Katsu, Yoshinao; Matsubara, Kazumi; Kohno, Satomi; Matsuda, Yoichi; Toriba, Michihisa; Oka, Kaori; Guillette, Louis; Ohta, Yasuhiko; Iguchi, Taisen.

In: Endocrinology, Vol. 151, 2010, p. 5710-5720.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular Cloning, Characterization, and Chromosome Mapping of Reptilian Estrogen Receptors

AU - Katsu, Yoshinao

AU - Matsubara, Kazumi

AU - Kohno, Satomi

AU - Matsuda, Yoichi

AU - Toriba, Michihisa

AU - Oka, Kaori

AU - Guillette, Louis

AU - Ohta, Yasuhiko

AU - Iguchi, Taisen

PY - 2010

Y1 - 2010

N2 - In many vertebrates, steroid hormones are essential for ovarian differentiation during a critical developmental stage as well as promoting the growth and differentiation of the adult female reproductive system. Although studies have been extensively conducted in mammals and a few fish, amphibians, and bird species, the molecular mechanisms of sex steroid hormone (estrogens) action have been poorly examined in reptiles. Here, we evaluate hormone receptor and ligand interactions in two species of snake, the Okinawa habu (Protobothrops flavoviridis, Viperidae) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae) after the isolation of cDNAs encoding estrogen receptor A (ESR1) and estrogen receptor B(ESR2). Using a transient transfection assay with mammalian cells, the transcriptional activity of reptilian (Okinawa habu, Japanese four-striped rat snake, American alligator, and Florida red-belly freshwater turtle) ESR1 and ESR2 was examined. All ESR proteins displayed estrogen-dependent activation of transcription via an estrogen-response element-containing promoter; however, the responsiveness to various estrogens was different. Further, we determined the chromosomal locations of the snake steroid hormone receptor genes. ESR1 and ESR2 genes were localized to the short and long arms of chromosome 1, respectively, whereas androgen receptor was localized to a pair of microchromosomes in the two snake species examined. These data provide basic tools that allow future studies examining receptor-ligand interactions and steroid endocrinology in snakes and also expands our knowledge of sex steroid hormone receptor evolution.

AB - In many vertebrates, steroid hormones are essential for ovarian differentiation during a critical developmental stage as well as promoting the growth and differentiation of the adult female reproductive system. Although studies have been extensively conducted in mammals and a few fish, amphibians, and bird species, the molecular mechanisms of sex steroid hormone (estrogens) action have been poorly examined in reptiles. Here, we evaluate hormone receptor and ligand interactions in two species of snake, the Okinawa habu (Protobothrops flavoviridis, Viperidae) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae) after the isolation of cDNAs encoding estrogen receptor A (ESR1) and estrogen receptor B(ESR2). Using a transient transfection assay with mammalian cells, the transcriptional activity of reptilian (Okinawa habu, Japanese four-striped rat snake, American alligator, and Florida red-belly freshwater turtle) ESR1 and ESR2 was examined. All ESR proteins displayed estrogen-dependent activation of transcription via an estrogen-response element-containing promoter; however, the responsiveness to various estrogens was different. Further, we determined the chromosomal locations of the snake steroid hormone receptor genes. ESR1 and ESR2 genes were localized to the short and long arms of chromosome 1, respectively, whereas androgen receptor was localized to a pair of microchromosomes in the two snake species examined. These data provide basic tools that allow future studies examining receptor-ligand interactions and steroid endocrinology in snakes and also expands our knowledge of sex steroid hormone receptor evolution.

U2 - 10.1210/en.2010-0356

DO - 10.1210/en.2010-0356

M3 - Article

VL - 151

SP - 5710

EP - 5720

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

ER -