Molecular diversity analysis and genetic mapping of pod shatter resistance loci in Brassica carinata L.

Rosy Raman, Yu Qiu, Neil Coombes, Jie Song, Andrzej Kilian, Harsh Raman

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Seed lost due to easy pod dehiscence at maturity (pod shatter) is a major problem in several members of Brassicaceae family. We investigated the level of pod shatter resistance in Ethiopian mustard (Brassica carinata) and identified quantitative trait loci (QTL) for targeted introgression of this trait in Ethiopian mustard and its close relatives of the genus Brassica. A set of 83 accessions of B. carinata, collected from the Australian Grains Genebank, was evaluated for pod shatter resistance based on pod rupture energy (RE). In comparison to B. napus (RE D 2.16 mJ), B. carinata accessions had higher RE values (2.53 to 20.82 mJ). A genetic linkage map of an F2 population from two contrasting B. carinata selections, BC73526 (shatter resistant with high RE) and BC73524 (shatter prone with low RE) comprising 300 individuals, was constructed using a set of 6,464 high quality DArTseq markers and subsequently used for QTL analysis. Genetic analysis of the F2 and F2:3 derived lines revealed five statistically significant QTL (LOD ≥ 3) that are linked with pod shatter resistance on chromosomes B1, B3, B8, and C5. Herein, we report for the first time, identification of genetic loci associated with pod shatter resistance in B. carinata. These characterized accessions would be useful in Brassica breeding programs for introgression of pod shatter resistance alleles in to elite breeding lines. Molecular markers would assist marker-assisted selection for tracing the introgression of resistant alleles. Our results suggest that the value of the germplasm collections can be harnessed through genetic and genomics tools.

    Original languageEnglish
    Article number1765
    Pages (from-to)1-12
    Number of pages12
    JournalFrontiers in Plant Science
    Volume8
    DOIs
    Publication statusPublished - 30 Nov 2017

    Fingerprint

    Brassica carinata
    chromosome mapping
    pods
    loci
    introgression
    energy
    quantitative trait loci
    Brassica
    alleles
    dehiscence
    germplasm conservation
    breeding lines
    Brassicaceae
    marker-assisted selection
    genetic techniques and protocols
    chromosomes
    genomics
    genetic markers
    breeding

    Cite this

    Raman, Rosy ; Qiu, Yu ; Coombes, Neil ; Song, Jie ; Kilian, Andrzej ; Raman, Harsh. / Molecular diversity analysis and genetic mapping of pod shatter resistance loci in Brassica carinata L. In: Frontiers in Plant Science. 2017 ; Vol. 8. pp. 1-12.
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    title = "Molecular diversity analysis and genetic mapping of pod shatter resistance loci in Brassica carinata L.",
    abstract = "Seed lost due to easy pod dehiscence at maturity (pod shatter) is a major problem in several members of Brassicaceae family. We investigated the level of pod shatter resistance in Ethiopian mustard (Brassica carinata) and identified quantitative trait loci (QTL) for targeted introgression of this trait in Ethiopian mustard and its close relatives of the genus Brassica. A set of 83 accessions of B. carinata, collected from the Australian Grains Genebank, was evaluated for pod shatter resistance based on pod rupture energy (RE). In comparison to B. napus (RE D 2.16 mJ), B. carinata accessions had higher RE values (2.53 to 20.82 mJ). A genetic linkage map of an F2 population from two contrasting B. carinata selections, BC73526 (shatter resistant with high RE) and BC73524 (shatter prone with low RE) comprising 300 individuals, was constructed using a set of 6,464 high quality DArTseq markers and subsequently used for QTL analysis. Genetic analysis of the F2 and F2:3 derived lines revealed five statistically significant QTL (LOD ≥ 3) that are linked with pod shatter resistance on chromosomes B1, B3, B8, and C5. Herein, we report for the first time, identification of genetic loci associated with pod shatter resistance in B. carinata. These characterized accessions would be useful in Brassica breeding programs for introgression of pod shatter resistance alleles in to elite breeding lines. Molecular markers would assist marker-assisted selection for tracing the introgression of resistant alleles. Our results suggest that the value of the germplasm collections can be harnessed through genetic and genomics tools.",
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    Molecular diversity analysis and genetic mapping of pod shatter resistance loci in Brassica carinata L. / Raman, Rosy; Qiu, Yu; Coombes, Neil; Song, Jie; Kilian, Andrzej; Raman, Harsh.

    In: Frontiers in Plant Science, Vol. 8, 1765, 30.11.2017, p. 1-12.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Raman, Rosy

    AU - Qiu, Yu

    AU - Coombes, Neil

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    AU - Kilian, Andrzej

    AU - Raman, Harsh

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    AB - Seed lost due to easy pod dehiscence at maturity (pod shatter) is a major problem in several members of Brassicaceae family. We investigated the level of pod shatter resistance in Ethiopian mustard (Brassica carinata) and identified quantitative trait loci (QTL) for targeted introgression of this trait in Ethiopian mustard and its close relatives of the genus Brassica. A set of 83 accessions of B. carinata, collected from the Australian Grains Genebank, was evaluated for pod shatter resistance based on pod rupture energy (RE). In comparison to B. napus (RE D 2.16 mJ), B. carinata accessions had higher RE values (2.53 to 20.82 mJ). A genetic linkage map of an F2 population from two contrasting B. carinata selections, BC73526 (shatter resistant with high RE) and BC73524 (shatter prone with low RE) comprising 300 individuals, was constructed using a set of 6,464 high quality DArTseq markers and subsequently used for QTL analysis. Genetic analysis of the F2 and F2:3 derived lines revealed five statistically significant QTL (LOD ≥ 3) that are linked with pod shatter resistance on chromosomes B1, B3, B8, and C5. Herein, we report for the first time, identification of genetic loci associated with pod shatter resistance in B. carinata. These characterized accessions would be useful in Brassica breeding programs for introgression of pod shatter resistance alleles in to elite breeding lines. Molecular markers would assist marker-assisted selection for tracing the introgression of resistant alleles. Our results suggest that the value of the germplasm collections can be harnessed through genetic and genomics tools.

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    KW - Molecular markers

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