Molecular evolution of Dmrt1 accompanies change of sex-determining mechanisms in reptilia

Daniel Janes, Christopher Organ, Denis O'Meally, Stephen SARRE, Arthur GEORGES, Jennifer Graves, Nicole Valenzuela, Robert Literman, Kim Rutherford, Neil Gemmell, John Iverson, Jeffrey Tamplin, Scott V. Edwards, Tariq EZAZ

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In reptiles, sex-determining mechanisms have evolved repeatedly and reversibly between genotypic and temperature-dependent sex determination. The gene Dmrt1 directs male determination in chicken (and presumably other birds), and regulates sex differentiation in animals as distantly related as fruit flies, nematodes and humans. Here, we show a consistent molecular difference in Dmrt1 between reptiles with genotypic and temperature dependent sex determination. Among 34 non-avian reptiles, a convergently evolved pair of amino acids encoded by sequence within exon 2 near the DM-binding domain of Dmrt1 distinguishes species with either type of sex determination. We suggest that this amino acid shift accompanied the evolution of genotypic sex determination from an ancestral condition of temperature-dependent sex determination at least three times among reptiles, as evident in turtles, birds and squamates. This novel hypothesis describes the evolution of sex-determining mechanisms as turnover events accompanied by one or two small mutations.
Original languageEnglish
Article number20140809
Pages (from-to)1-4
Number of pages4
JournalBiology Letters
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 2014

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Reptiles
sex reversal
Molecular Evolution
Reptilia
gender
reptiles
Temperature
Birds
Sex Differentiation
temperature
amino acids
Turtles
sexual development
birds
Squamata
fruit flies
Diptera
turtles
exons
Amino Acid Sequence

Cite this

Janes, Daniel ; Organ, Christopher ; O'Meally, Denis ; SARRE, Stephen ; GEORGES, Arthur ; Graves, Jennifer ; Valenzuela, Nicole ; Literman, Robert ; Rutherford, Kim ; Gemmell, Neil ; Iverson, John ; Tamplin, Jeffrey ; Edwards, Scott V. ; EZAZ, Tariq. / Molecular evolution of Dmrt1 accompanies change of sex-determining mechanisms in reptilia. In: Biology Letters. 2014 ; Vol. 10, No. 12. pp. 1-4.
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abstract = "In reptiles, sex-determining mechanisms have evolved repeatedly and reversibly between genotypic and temperature-dependent sex determination. The gene Dmrt1 directs male determination in chicken (and presumably other birds), and regulates sex differentiation in animals as distantly related as fruit flies, nematodes and humans. Here, we show a consistent molecular difference in Dmrt1 between reptiles with genotypic and temperature dependent sex determination. Among 34 non-avian reptiles, a convergently evolved pair of amino acids encoded by sequence within exon 2 near the DM-binding domain of Dmrt1 distinguishes species with either type of sex determination. We suggest that this amino acid shift accompanied the evolution of genotypic sex determination from an ancestral condition of temperature-dependent sex determination at least three times among reptiles, as evident in turtles, birds and squamates. This novel hypothesis describes the evolution of sex-determining mechanisms as turnover events accompanied by one or two small mutations.",
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author = "Daniel Janes and Christopher Organ and Denis O'Meally and Stephen SARRE and Arthur GEORGES and Jennifer Graves and Nicole Valenzuela and Robert Literman and Kim Rutherford and Neil Gemmell and John Iverson and Jeffrey Tamplin and Edwards, {Scott V.} and Tariq EZAZ",
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Janes, D, Organ, C, O'Meally, D, SARRE, S, GEORGES, A, Graves, J, Valenzuela, N, Literman, R, Rutherford, K, Gemmell, N, Iverson, J, Tamplin, J, Edwards, SV & EZAZ, T 2014, 'Molecular evolution of Dmrt1 accompanies change of sex-determining mechanisms in reptilia', Biology Letters, vol. 10, no. 12, 20140809, pp. 1-4. https://doi.org/10.1098/rsbl.2014.0809

Molecular evolution of Dmrt1 accompanies change of sex-determining mechanisms in reptilia. / Janes, Daniel; Organ, Christopher; O'Meally, Denis; SARRE, Stephen; GEORGES, Arthur; Graves, Jennifer; Valenzuela, Nicole; Literman, Robert; Rutherford, Kim; Gemmell, Neil; Iverson, John; Tamplin, Jeffrey; Edwards, Scott V.; EZAZ, Tariq.

In: Biology Letters, Vol. 10, No. 12, 20140809, 12.2014, p. 1-4.

Research output: Contribution to journalArticle

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AU - Janes, Daniel

AU - Organ, Christopher

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AU - SARRE, Stephen

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AU - Graves, Jennifer

AU - Valenzuela, Nicole

AU - Literman, Robert

AU - Rutherford, Kim

AU - Gemmell, Neil

AU - Iverson, John

AU - Tamplin, Jeffrey

AU - Edwards, Scott V.

AU - EZAZ, Tariq

N1 - © 2014 The Author(s) Published by the Royal Society. All rights reserved.

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KW - Convergence

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