Molecular profiling of behavioural development: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)

R Kucharski, R Maleszka

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22 Citations (Scopus)

Abstract

In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.
Original languageEnglish
Pages (from-to)92-101
Number of pages10
JournalMolecular Brain Research
Volume99
Issue number2
DOIs
Publication statusPublished - 28 Mar 2002
Externally publishedYes

Cite this

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title = "Molecular profiling of behavioural development: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)",
abstract = "In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.",
keywords = "second messenger, calcium mobilisation, Drosophila",
author = "R Kucharski and R Maleszka",
year = "2002",
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doi = "10.1016/S0169-328X(01)00325-4",
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T1 - Molecular profiling of behavioural development: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)

AU - Kucharski, R

AU - Maleszka, R

PY - 2002/3/28

Y1 - 2002/3/28

N2 - In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.

AB - In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.

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KW - Drosophila

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