Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 92-101 |
| Number of pages | 10 |
| Journal | Molecular Brain Research |
| Volume | 99 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 28 Mar 2002 |
| Externally published | Yes |
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Molecular profiling of behavioural development: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera). / Kucharski, R; Maleszka, R.
In: Molecular Brain Research, Vol. 99, No. 2, 28.03.2002, p. 92-101.Research output: Contribution to journal › Article
TY - JOUR
T1 - Molecular profiling of behavioural development: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)
AU - Kucharski, R
AU - Maleszka, R
PY - 2002/3/28
Y1 - 2002/3/28
N2 - In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.
AB - In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1,4,5-trisphosphate (IP ) 3-kinase, a key enzyme in the IP -mediated signalling cascade, is differentially expressed in brains of naive, 3 3 newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP K locus and a 39 3 flanking gene similar to a transcription factor NFR-k-B. The IP K locus gives rise to three differentially expressed major transcripts 3 produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees.
KW - second messenger
KW - calcium mobilisation
KW - Drosophila
U2 - 10.1016/S0169-328X(01)00325-4
DO - 10.1016/S0169-328X(01)00325-4
M3 - Article
VL - 99
SP - 92
EP - 101
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -