Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

Marta Trevisan, Veronica Di Antonio, Annalisa Radeghieri, Giorgio Palù, Reena Ghildyal, Gualtiero Alvisi

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Abstract

Respiratory syncytial virus (RSV) is an important human pathogen, which infects respiratory tract epithelial cells causing bronchiolitis and pneumonia in children and the elderly. Recent studies have linked RSV matrix (M) ability to self-interaction and viral budding. However, RSV M has been crystalized both as a monomer and a dimer, and no formal proof exists to date that it forms dimers in cells. Here, by using a combination of confocal laser scanning microscopy and bioluminescent resonant energy transfer applied to differently tagged deletion mutants of RSV M, we show that the protein can self-interact in living mammalian cells and that both the N and C-terminus of the protein are strictly required for the process, consistent with the reported dimeric crystal structure.

Original languageEnglish
Article number109
Pages (from-to)109-1 - 109-13
Number of pages13
JournalViruses
Volume10
Issue number3
DOIs
Publication statusPublished - 3 Mar 2018

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