Monitoring on-line of extracellular gamma-amino-4-butyric acid using microdialysis coupled to immunosensor analysis

C. J. Cook

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A method is described for the measurement of gamma-amino-4- butyric acid (GABA) within a microdialysis probe using an antibody- linked assay. A monoclonal antibody for GABA provides the specificity of measurement and these antibodies are affixed on a working platinum electrode within the probe. Determination of bound GABA is performed via an indirect assessment of competitive ligand also bound, and conjugated to horseradish peroxidase which is activated and measured as current change. Using this probe directly for extracellular measurements in the somatosensory cortex of sheep compared favorably to the use of this probe on dialysate emerging from a classical microdialysis probe suggesting that it could be used directly in vivo. The probe has a fast response time (>90% of maximum response within 30 s of start of analysis) and high sensitivity (< 0.5 μmol/l) and can acquire data every 2 min. It is stable over a period of time in vivo (>48 h) and is regenerable. The probe design offers on-line measurement, with rapid time resolution of substances not amenable to enzyme-based amperometric measurement.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalJournal of Neuroscience Methods
Volume82
Issue number2
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Butyric Acid
Microdialysis
Somatosensory Cortex
Antibody Specificity
Dialysis Solutions
Horseradish Peroxidase
Platinum
Reaction Time
Sheep
Electrodes
Monoclonal Antibodies
Ligands
Antibodies
Enzymes

Cite this

@article{79594dbc6fff4b749782d828d5a3251e,
title = "Monitoring on-line of extracellular gamma-amino-4-butyric acid using microdialysis coupled to immunosensor analysis",
abstract = "A method is described for the measurement of gamma-amino-4- butyric acid (GABA) within a microdialysis probe using an antibody- linked assay. A monoclonal antibody for GABA provides the specificity of measurement and these antibodies are affixed on a working platinum electrode within the probe. Determination of bound GABA is performed via an indirect assessment of competitive ligand also bound, and conjugated to horseradish peroxidase which is activated and measured as current change. Using this probe directly for extracellular measurements in the somatosensory cortex of sheep compared favorably to the use of this probe on dialysate emerging from a classical microdialysis probe suggesting that it could be used directly in vivo. The probe has a fast response time (>90{\%} of maximum response within 30 s of start of analysis) and high sensitivity (< 0.5 μmol/l) and can acquire data every 2 min. It is stable over a period of time in vivo (>48 h) and is regenerable. The probe design offers on-line measurement, with rapid time resolution of substances not amenable to enzyme-based amperometric measurement.",
keywords = "Epileptiform activity, GABA, Immunosensor, Microdialysis",
author = "Cook, {C. J.}",
year = "1998",
doi = "10.1016/S0165-0270(98)00046-6",
language = "English",
volume = "82",
pages = "145--150",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

}

Monitoring on-line of extracellular gamma-amino-4-butyric acid using microdialysis coupled to immunosensor analysis. / Cook, C. J.

In: Journal of Neuroscience Methods, Vol. 82, No. 2, 1998, p. 145-150.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monitoring on-line of extracellular gamma-amino-4-butyric acid using microdialysis coupled to immunosensor analysis

AU - Cook, C. J.

PY - 1998

Y1 - 1998

N2 - A method is described for the measurement of gamma-amino-4- butyric acid (GABA) within a microdialysis probe using an antibody- linked assay. A monoclonal antibody for GABA provides the specificity of measurement and these antibodies are affixed on a working platinum electrode within the probe. Determination of bound GABA is performed via an indirect assessment of competitive ligand also bound, and conjugated to horseradish peroxidase which is activated and measured as current change. Using this probe directly for extracellular measurements in the somatosensory cortex of sheep compared favorably to the use of this probe on dialysate emerging from a classical microdialysis probe suggesting that it could be used directly in vivo. The probe has a fast response time (>90% of maximum response within 30 s of start of analysis) and high sensitivity (< 0.5 μmol/l) and can acquire data every 2 min. It is stable over a period of time in vivo (>48 h) and is regenerable. The probe design offers on-line measurement, with rapid time resolution of substances not amenable to enzyme-based amperometric measurement.

AB - A method is described for the measurement of gamma-amino-4- butyric acid (GABA) within a microdialysis probe using an antibody- linked assay. A monoclonal antibody for GABA provides the specificity of measurement and these antibodies are affixed on a working platinum electrode within the probe. Determination of bound GABA is performed via an indirect assessment of competitive ligand also bound, and conjugated to horseradish peroxidase which is activated and measured as current change. Using this probe directly for extracellular measurements in the somatosensory cortex of sheep compared favorably to the use of this probe on dialysate emerging from a classical microdialysis probe suggesting that it could be used directly in vivo. The probe has a fast response time (>90% of maximum response within 30 s of start of analysis) and high sensitivity (< 0.5 μmol/l) and can acquire data every 2 min. It is stable over a period of time in vivo (>48 h) and is regenerable. The probe design offers on-line measurement, with rapid time resolution of substances not amenable to enzyme-based amperometric measurement.

KW - Epileptiform activity

KW - GABA

KW - Immunosensor

KW - Microdialysis

UR - http://www.scopus.com/inward/record.url?scp=0032145216&partnerID=8YFLogxK

U2 - 10.1016/S0165-0270(98)00046-6

DO - 10.1016/S0165-0270(98)00046-6

M3 - Article

VL - 82

SP - 145

EP - 150

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -