Monitoring salivary testosterone and cortisol concentrations across an international sports competition:

Data comparison using two enzyme immunoassays and two sample preparations

Blair T Crewther, E. Al-Dujaili, N.F. Smail, S. Anastasova, Liam P Kilduff, C.J. Cook

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations. Design and methods: A group of male athletes (n=19) provided a saliva sample the morning before and one day after (24h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CME) and an in-house method (IHE). Raw samples (no extraction procedure) were also tested using the commercial kit (CMR). Results: There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IHE>CME>CMR) and C (CMR>IHE and CME) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IHE and CME measures of salivary T (r=0.93-0.97) and C (r=0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r=0.93-0.96). Conclusions: The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen. © 2012 The Canadian Society of Clinical Chemists.
Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalClinical Biochemistry
Volume46
Issue number4-5
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Sports
Immunoenzyme Techniques
Hydrocortisone
Testosterone
Monitoring
Enzymes
Hormones
Football
Saliva
Athletes

Cite this

Crewther, Blair T ; Al-Dujaili, E. ; Smail, N.F. ; Anastasova, S. ; Kilduff, Liam P ; Cook, C.J. / Monitoring salivary testosterone and cortisol concentrations across an international sports competition: Data comparison using two enzyme immunoassays and two sample preparations. In: Clinical Biochemistry. 2013 ; Vol. 46, No. 4-5. pp. 354-358.
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Monitoring salivary testosterone and cortisol concentrations across an international sports competition: Data comparison using two enzyme immunoassays and two sample preparations. / Crewther, Blair T; Al-Dujaili, E.; Smail, N.F.; Anastasova, S.; Kilduff, Liam P; Cook, C.J.

In: Clinical Biochemistry, Vol. 46, No. 4-5, 2013, p. 354-358.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monitoring salivary testosterone and cortisol concentrations across an international sports competition:

T2 - Data comparison using two enzyme immunoassays and two sample preparations

AU - Crewther, Blair T

AU - Al-Dujaili, E.

AU - Smail, N.F.

AU - Anastasova, S.

AU - Kilduff, Liam P

AU - Cook, C.J.

N1 - Cited By :6 Export Date: 25 May 2017

PY - 2013

Y1 - 2013

N2 - Objectives: Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations. Design and methods: A group of male athletes (n=19) provided a saliva sample the morning before and one day after (24h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CME) and an in-house method (IHE). Raw samples (no extraction procedure) were also tested using the commercial kit (CMR). Results: There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IHE>CME>CMR) and C (CMR>IHE and CME) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IHE and CME measures of salivary T (r=0.93-0.97) and C (r=0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r=0.93-0.96). Conclusions: The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen. © 2012 The Canadian Society of Clinical Chemists.

AB - Objectives: Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations. Design and methods: A group of male athletes (n=19) provided a saliva sample the morning before and one day after (24h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CME) and an in-house method (IHE). Raw samples (no extraction procedure) were also tested using the commercial kit (CMR). Results: There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IHE>CME>CMR) and C (CMR>IHE and CME) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IHE and CME measures of salivary T (r=0.93-0.97) and C (r=0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r=0.93-0.96). Conclusions: The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen. © 2012 The Canadian Society of Clinical Chemists.

KW - Athletes

KW - Elite sport

KW - Saliva

KW - Steroids

KW - Validation

U2 - 10.1016/j.clinbiochem.2012.11.019

DO - 10.1016/j.clinbiochem.2012.11.019

M3 - Article

VL - 46

SP - 354

EP - 358

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 4-5

ER -