Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka

Lasanthika D. Thewarage; Duminda S. B. Dissanayake; Udari S. Perera; Anura T. Bandara; S. Wickramasinghe; B. V. P. Perera; R. P. V. J. Rajapakse

doi:10.2478/s11686-020-00193-3

Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka

Lasanthika D. Thewarage, Duminda S. B. Dissanayake, Udari S. Perera, Anura T. Bandara, S. Wickramasinghe, B. V. P. Perera, R. P. V. J. Rajapakse

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Abstract

Purpose
The aim of the present study was to carry out a detailed study of morphological features and to determine the phylogenetic position of Parabronema smithii (Cobbold, 1882) found in wild elephants in Sri Lanka.

Methods
Adult worms were collected from stomach ulcers at postmortem examination of wild elephants in the Udawalawe National Park, Sri Lanka. The detailed morphology of P. smithii was studied using light microscopy and, for the first time, scanning electron microscopy. Fifteen morphological characteristics were investigated. The phylogenetic analysis was conducted using the second internal transcribed spacer region (ITS2), and portions of the large subunit ribosomal DNA (28S) and cytochrome c oxidase subunit 1 (cox1). Furthermore, the present study provides a comparison of morphology and morphometrics of Parabronema species that occur in different hosts.

Conclusion
Parabronema smithii isolated from wild elephants exhibited the key morphological features. Phylogenetic analysis of selected genes revealed that P. smithii is closely associated with P. skrjabini and Habronema spp. Findings of the present study enhance our understanding of the biology and taxonomy of P. smithii in wild elephant in Sri Lanka and will contribute to future phylogeographic studies.

Original language	English
Pages (from-to)	504-517
Number of pages	14
Journal	Acta Parasitologica
Volume	65
Issue number	2
DOIs	https://doi.org/10.2478/s11686-020-00193-3
Publication status	Published - 1 Jun 2020

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10.2478/s11686-020-00193-3

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Thewarage, L. D., Dissanayake, D. S. B., Perera, U. S., Bandara, A. T., Wickramasinghe, S., Perera, B. V. P., & Rajapakse, R. P. V. J. (2020). Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka. Acta Parasitologica, 65(2), 504-517. https://doi.org/10.2478/s11686-020-00193-3

@article{bfd574ed374f4feaa1b641199ac6ffa7,

title = "Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka",

abstract = "PurposeThe aim of the present study was to carry out a detailed study of morphological features and to determine the phylogenetic position of Parabronema smithii (Cobbold, 1882) found in wild elephants in Sri Lanka.MethodsAdult worms were collected from stomach ulcers at postmortem examination of wild elephants in the Udawalawe National Park, Sri Lanka. The detailed morphology of P. smithii was studied using light microscopy and, for the first time, scanning electron microscopy. Fifteen morphological characteristics were investigated. The phylogenetic analysis was conducted using the second internal transcribed spacer region (ITS2), and portions of the large subunit ribosomal DNA (28S) and cytochrome c oxidase subunit 1 (cox1). Furthermore, the present study provides a comparison of morphology and morphometrics of Parabronema species that occur in different hosts.ConclusionParabronema smithii isolated from wild elephants exhibited the key morphological features. Phylogenetic analysis of selected genes revealed that P. smithii is closely associated with P. skrjabini and Habronema spp. Findings of the present study enhance our understanding of the biology and taxonomy of P. smithii in wild elephant in Sri Lanka and will contribute to future phylogeographic studies.",

keywords = "Parabronema smithii, Asian elephants, Sri Lanka, Phylogenetic analysis, Taxonomy",

author = "Thewarage, {Lasanthika D.} and Dissanayake, {Duminda S. B.} and Perera, {Udari S.} and Bandara, {Anura T.} and S. Wickramasinghe and Perera, {B. V. P.} and Rajapakse, {R. P. V. J.}",

note = "Funding Information: This work was supported by National Research Council of Sri Lanka. Publisher Copyright: {\textcopyright} 2020, Witold Stefa{\'n}ski Institute of Parasitology, Polish Academy of Sciences.",

year = "2020",

month = jun,

day = "1",

doi = "10.2478/s11686-020-00193-3",

language = "English",

volume = "65",

pages = "504--517",

journal = "Acta Parasitologica",

issn = "1230-2821",

publisher = "De Gruyter",

number = "2",

}

Thewarage, LD, Dissanayake, DSB, Perera, US, Bandara, AT, Wickramasinghe, S, Perera, BVP & Rajapakse, RPVJ 2020, 'Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka', Acta Parasitologica, vol. 65, no. 2, pp. 504-517. https://doi.org/10.2478/s11686-020-00193-3

Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka. / Thewarage, Lasanthika D.; Dissanayake, Duminda S. B.; Perera, Udari S. et al.
In: Acta Parasitologica, Vol. 65, No. 2, 01.06.2020, p. 504-517.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka

AU - Thewarage, Lasanthika D.

AU - Dissanayake, Duminda S. B.

AU - Perera, Udari S.

AU - Bandara, Anura T.

AU - Wickramasinghe, S.

AU - Perera, B. V. P.

AU - Rajapakse, R. P. V. J.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - PurposeThe aim of the present study was to carry out a detailed study of morphological features and to determine the phylogenetic position of Parabronema smithii (Cobbold, 1882) found in wild elephants in Sri Lanka.MethodsAdult worms were collected from stomach ulcers at postmortem examination of wild elephants in the Udawalawe National Park, Sri Lanka. The detailed morphology of P. smithii was studied using light microscopy and, for the first time, scanning electron microscopy. Fifteen morphological characteristics were investigated. The phylogenetic analysis was conducted using the second internal transcribed spacer region (ITS2), and portions of the large subunit ribosomal DNA (28S) and cytochrome c oxidase subunit 1 (cox1). Furthermore, the present study provides a comparison of morphology and morphometrics of Parabronema species that occur in different hosts.ConclusionParabronema smithii isolated from wild elephants exhibited the key morphological features. Phylogenetic analysis of selected genes revealed that P. smithii is closely associated with P. skrjabini and Habronema spp. Findings of the present study enhance our understanding of the biology and taxonomy of P. smithii in wild elephant in Sri Lanka and will contribute to future phylogeographic studies.

AB - PurposeThe aim of the present study was to carry out a detailed study of morphological features and to determine the phylogenetic position of Parabronema smithii (Cobbold, 1882) found in wild elephants in Sri Lanka.MethodsAdult worms were collected from stomach ulcers at postmortem examination of wild elephants in the Udawalawe National Park, Sri Lanka. The detailed morphology of P. smithii was studied using light microscopy and, for the first time, scanning electron microscopy. Fifteen morphological characteristics were investigated. The phylogenetic analysis was conducted using the second internal transcribed spacer region (ITS2), and portions of the large subunit ribosomal DNA (28S) and cytochrome c oxidase subunit 1 (cox1). Furthermore, the present study provides a comparison of morphology and morphometrics of Parabronema species that occur in different hosts.ConclusionParabronema smithii isolated from wild elephants exhibited the key morphological features. Phylogenetic analysis of selected genes revealed that P. smithii is closely associated with P. skrjabini and Habronema spp. Findings of the present study enhance our understanding of the biology and taxonomy of P. smithii in wild elephant in Sri Lanka and will contribute to future phylogeographic studies.

KW - Parabronema smithii

KW - Asian elephants

KW - Sri Lanka

KW - Phylogenetic analysis

KW - Taxonomy

UR - http://www.scopus.com/inward/record.url?scp=85081563216&partnerID=8YFLogxK

U2 - 10.2478/s11686-020-00193-3

DO - 10.2478/s11686-020-00193-3

M3 - Article

SN - 1230-2821

VL - 65

SP - 504

EP - 517

JO - Acta Parasitologica

JF - Acta Parasitologica

IS - 2

ER -

Morphology and Molecular Characterization of Parabronema smithii (Cobbold, 1882) (Nematoda: Habronematidae) from Wild Asian Elephant (Elephas maximus maximus) of Sri Lanka

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