Neutrophil oxidative activity is differentially affected by exercise intensity and type

David B. Pyne, John A. Smith, Mark S. Baker, Richard D. Telford, Maurice J. Weidemann

    Research output: Contribution to journalArticle

    45 Citations (Scopus)

    Abstract

    The differential effects of exercise intensity and type on neutrophil activation were assessed in eight well-trained male runners. Each subject undertook, on different days, three separate 40 min interval (8 x 5 min) treadmill bouts: an intense uphill run (90% VO2 max), a moderate-intensity near-level run and an eccentrically-biased downhill run (both at 52% VO2 max). Blood granulocyte count increased (p< 0.05) after all three treadmill bouts (range 25-108%). Chemiluminescence activity of isolated neutrophils decreased (p< 0.05) immediately after (-58%) and 1-h after (-72%) uphill running, but became significantly elevated (p< 0.05) at 6-h after the near- level (+71%) and downhill (+84%) runs. The ability of neutrophils to release the superoxide anion radical was reduced (p< 0.05) immediately after near- level (-29%) and uphill (-21%) running in cells stimulated with opsonized zymosan. Epinephrine concentration increased by 430% (p=0.01) after uphill but not with near-level or downhill running. The plasma concentration of elastase increased (p< 0.05) immediately after uphill and near-level running, and one hour after uphill running. These results suggest that a population of neutrophils mobilised into the circulation became directly activated in response to exercise, and that neutrophil oxidative activity is affected differentially by both the intensity and type of exercise undertaken.

    Original languageEnglish
    Pages (from-to)44-54
    Number of pages11
    JournalJournal of Science and Medicine in Sport
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - 2000

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    Neutrophils
    Superoxides
    Neutrophil Activation
    Zymosan
    Pancreatic Elastase
    Luminescence
    Granulocytes
    Epinephrine
    Population

    Cite this

    Pyne, David B. ; Smith, John A. ; Baker, Mark S. ; Telford, Richard D. ; Weidemann, Maurice J. / Neutrophil oxidative activity is differentially affected by exercise intensity and type. In: Journal of Science and Medicine in Sport. 2000 ; Vol. 3, No. 1. pp. 44-54.
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    Neutrophil oxidative activity is differentially affected by exercise intensity and type. / Pyne, David B.; Smith, John A.; Baker, Mark S.; Telford, Richard D.; Weidemann, Maurice J.

    In: Journal of Science and Medicine in Sport, Vol. 3, No. 1, 2000, p. 44-54.

    Research output: Contribution to journalArticle

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