New Chiral Bonded Monolithic Capillary Columns for Enantioselective Nano-LC Separations

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The increasing requirement to reduce the total analysis times, reagent consumption together with the handling of smaller complex samples; has led to recent progress and research into micro-total analysis systems (-TAS) [1-4]. Recently, this trend for miniaturization has been applied to other analytical fields such as micro and nano-HPLC, proteomics, peptide analysis and bio-analytical applications [2]. A prerequisite for the success of such applications is the accurate and reliable delivery of the mobile phase at L min-1 or nL min-1 flow rates under applied backpressures and finding the suitable column that can operate under micro or nano flow. The development of micro-pumps and fluid-handling systems capable of meeting the flow demanding criteria has made nano-LC an environmentally benign technique for performing enantioseparations in capillary format. The stationary phases in capillary LC have been greatly developed due to the diverse demands of microscale separations [2]. Monolithic columns are described as the integrated continuous porous separation media for chromatographic separation sciences [4]. They have emerged as an alternative to traditional packed-bed columns for high efficiency separations in HPLC due to their small-sized skeletons and wide through-pores [1]. In this presentation, monolithic capillary columns were synthesized by in situ preparation of polymer or silica-based monoliths with various specific functionalities arising from the versatile in situ chemical reactions and/or post-modifications [5]. Most importantly, the in situ prepared monoliths are chemically attached onto the inner wall of capillaries, and no retaining frits are required to support the monolithic matrices. The new bonded chiral monolithic phases are investigated for the enantioselective separation of different classes of chemicals and pharmaceutical-related compounds. The advantages of the nano-scale enantioselective separation will be discussed
Original languageEnglish
Title of host publication40th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2013)
EditorsMichael C. Breadmore, Deirdre Cabooter, John Dorsey, Paola Dugo
Place of PublicationCanberra
Number of pages1
Publication statusPublished - 2013
EventHPLC 2013 - Hobart, Hobart, Australia
Duration: 18 Nov 201221 Nov 2012

Publication series

NameJournal of Chromatography A
ISSN (Print)0021-9673


ConferenceHPLC 2013


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