Non-Homologous Sex Chromosomes in Two Geckos (Gekkonidae: Gekkota) with Female Heterogamety

Kazumi MATSUBARA, Tony Gamble, Yoichi Matsuda, David Zarkower, Stephen SARRE, Arthur GEORGES, Jennifer Graves, Tariq EZAZ

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Evaluating homology between the sex chromosomes of different species is an important first step in deducing the origins and evolution of sex-determining mechanisms in a clade. Here, we describe the preparation of Z and W chromosome paints via chromosome microdissection from the Australian marbled gecko (Christinus marmoratus) and their subsequent use in evaluating sex chromosome homology with the ZW chromosomes of the Kwangsi gecko (Gekko hokouensis) from eastern Asia. We show that the ZW sex chromosomes of C. marmoratus and G. hokouensis are not homologous and represent independent origins of female heterogamety within the Gekkonidae. We also show that the C. marmoratus Z and W chromosomes are genetically similar to each other as revealed by C-banding, comparative genomic hybridization, and the reciprocal painting of Z and W chromosome probes. This implies that sex chromosomes in C. marmoratus are at an early stage of differentiation, suggesting a recent origin.
    Original languageEnglish
    Pages (from-to)251-258
    Number of pages8
    JournalCytogenetic and Genome Research
    Volume143
    Issue number4
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Sex Chromosomes
    Lizards
    Chromosomes
    Chromosomes, Human, 6-12 and X
    Chromosomes, Human, 21-22 and Y
    Microdissection
    Paintings
    Paint
    Comparative Genomic Hybridization
    Far East

    Cite this

    MATSUBARA, Kazumi ; Gamble, Tony ; Matsuda, Yoichi ; Zarkower, David ; SARRE, Stephen ; GEORGES, Arthur ; Graves, Jennifer ; EZAZ, Tariq. / Non-Homologous Sex Chromosomes in Two Geckos (Gekkonidae: Gekkota) with Female Heterogamety. In: Cytogenetic and Genome Research. 2014 ; Vol. 143, No. 4. pp. 251-258.
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    abstract = "Evaluating homology between the sex chromosomes of different species is an important first step in deducing the origins and evolution of sex-determining mechanisms in a clade. Here, we describe the preparation of Z and W chromosome paints via chromosome microdissection from the Australian marbled gecko (Christinus marmoratus) and their subsequent use in evaluating sex chromosome homology with the ZW chromosomes of the Kwangsi gecko (Gekko hokouensis) from eastern Asia. We show that the ZW sex chromosomes of C. marmoratus and G. hokouensis are not homologous and represent independent origins of female heterogamety within the Gekkonidae. We also show that the C. marmoratus Z and W chromosomes are genetically similar to each other as revealed by C-banding, comparative genomic hybridization, and the reciprocal painting of Z and W chromosome probes. This implies that sex chromosomes in C. marmoratus are at an early stage of differentiation, suggesting a recent origin.",
    author = "Kazumi MATSUBARA and Tony Gamble and Yoichi Matsuda and David Zarkower and Stephen SARRE and Arthur GEORGES and Jennifer Graves and Tariq EZAZ",
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    Non-Homologous Sex Chromosomes in Two Geckos (Gekkonidae: Gekkota) with Female Heterogamety. / MATSUBARA, Kazumi; Gamble, Tony; Matsuda, Yoichi; Zarkower, David; SARRE, Stephen; GEORGES, Arthur; Graves, Jennifer; EZAZ, Tariq.

    In: Cytogenetic and Genome Research, Vol. 143, No. 4, 2014, p. 251-258.

    Research output: Contribution to journalArticle

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    AU - MATSUBARA, Kazumi

    AU - Gamble, Tony

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    AU - Zarkower, David

    AU - SARRE, Stephen

    AU - GEORGES, Arthur

    AU - Graves, Jennifer

    AU - EZAZ, Tariq

    PY - 2014

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    AB - Evaluating homology between the sex chromosomes of different species is an important first step in deducing the origins and evolution of sex-determining mechanisms in a clade. Here, we describe the preparation of Z and W chromosome paints via chromosome microdissection from the Australian marbled gecko (Christinus marmoratus) and their subsequent use in evaluating sex chromosome homology with the ZW chromosomes of the Kwangsi gecko (Gekko hokouensis) from eastern Asia. We show that the ZW sex chromosomes of C. marmoratus and G. hokouensis are not homologous and represent independent origins of female heterogamety within the Gekkonidae. We also show that the C. marmoratus Z and W chromosomes are genetically similar to each other as revealed by C-banding, comparative genomic hybridization, and the reciprocal painting of Z and W chromosome probes. This implies that sex chromosomes in C. marmoratus are at an early stage of differentiation, suggesting a recent origin.

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