Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications

Dianne Gleeson, Andrea Byrom, Robyn Howitt

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.
    Original languageEnglish
    Pages (from-to)356-359
    Number of pages4
    JournalNew Zealand Journal of Ecology
    Volume34
    Publication statusPublished - 2010

    Fingerprint

    Mustela erminea
    genotyping
    hair
    DNA
    trapping
    capture method
    trichomes
    methodology
    population density
    genotype
    sampling
    loci
    hair follicles
    method
    animal
    Fagus
    traps
    pests
    microsatellite repeats

    Cite this

    @article{d89847fc512647afb415e17fcdc8d43c,
    title = "Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications",
    abstract = "Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.",
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    language = "English",
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    Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications. / Gleeson, Dianne; Byrom, Andrea; Howitt, Robyn.

    In: New Zealand Journal of Ecology, Vol. 34, 2010, p. 356-359.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications

    AU - Gleeson, Dianne

    AU - Byrom, Andrea

    AU - Howitt, Robyn

    PY - 2010

    Y1 - 2010

    N2 - Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.

    AB - Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.

    M3 - Article

    VL - 34

    SP - 356

    EP - 359

    JO - New Zealand Journal of Ecology

    JF - New Zealand Journal of Ecology

    SN - 0077-9946

    ER -