Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications

Dianne Gleeson, Andrea Byrom, Robyn Howitt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.
Original languageEnglish
Pages (from-to)356-359
Number of pages4
JournalNew Zealand Journal of Ecology
Volume34
Publication statusPublished - 2010

Fingerprint

Mustela erminea
genotyping
hair
DNA
trapping
capture method
trichomes
methodology
population density
genotype
sampling
loci
hair follicles
method
animal
Fagus
traps
pests
microsatellite repeats

Cite this

@article{d89847fc512647afb415e17fcdc8d43c,
title = "Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications",
abstract = "Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.",
author = "Dianne Gleeson and Andrea Byrom and Robyn Howitt",
year = "2010",
language = "English",
volume = "34",
pages = "356--359",
journal = "New Zealand Journal of Ecology",
issn = "0077-9946",
publisher = "New Zealand Ecological Society",

}

Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications. / Gleeson, Dianne; Byrom, Andrea; Howitt, Robyn.

In: New Zealand Journal of Ecology, Vol. 34, 2010, p. 356-359.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Non-invasive methods for genotyping of stoats (Mustela erminea) in New Zealand: potential for field applications

AU - Gleeson, Dianne

AU - Byrom, Andrea

AU - Howitt, Robyn

PY - 2010

Y1 - 2010

N2 - Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.

AB - Stoats (Mustela erminea) are a significant pest in New Zealand. A critical aspect of their management is the ability to identify individuals in order to estimate abundance or to determine the origin of residual animals after control, particularly as the trap-shy nature of stoats reduces the utility of trapping to gain this information. We investigated noninvasive ‘capture’ methods as an alternative to live-trapping or removal methods for estimating stoat abundance. First we determined whether sufficient variability exists at six microsatellite DNA loci to reliably identify individuals in the potentially bottlenecked, introduced stoat populations of New Zealand. In December 2001 we conducted a 7-night pilot field experiment using a modified hair-tube design, where we obtained a total of 64 hair samples. Sufficient DNA was extracted from 3–6 hair follicles to genotype a total of 51 samples. DNA quality declined if samples were left in the field for several nights before being collected, and daily checks proved best for maximising the quality of DNA obtained, while minimising the risk of multiple ‘captures’ of stoats. Conclusions were that non-invasive molecular sampling is likely to be a viable technique for estimating population density of stoats in New Zealand beech forest but that additional variable loci are required.

M3 - Article

VL - 34

SP - 356

EP - 359

JO - New Zealand Journal of Ecology

JF - New Zealand Journal of Ecology

SN - 0077-9946

ER -