Novel cuticular proteins revealed by the honey bee genome

R. Kucharski, J. Maleszka, R. Maleszka

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

With the completion of the honey bee genome project, a transition is now occurring from the acquisition of gene sequence to understanding the role and context of gene products within the genome. Here we annotated and characterised a cluster of three genes in a GC-rich 11 kb genomic region on the linkage group 4 encoding highly hydrophobic polypeptides (named apidermins; APD 1–3) containing both sequence motifs characteristic of cuticular proteins and distinctly novel features. Five amino acids, Ala, Gly, Leu, Pro and Val, account for 74–86% of their respective sequences with Ala being the most abundant residue (at least 30% of each peptide). A conserved tetra-peptide AAPA/V is found in all three proteins, but none has the 0 R and R0 signature implicated in chitin binding. Two proteins, APD-1 and APD-2, contain an arginine-rich motif RERR in short non-hydrophobic stretches near the N-terminal of mature proteins and in both proteins tryptophan is the C-terminal residue. All three genes are spliced and highly expressed in a defined spatiotemporal pattern. apd-1 is expressed in the exoskeletal epidermis, but only during a restricted period of a few days of late pupal and early adult life when the cuticle becomes dark. APD2 appears to be a protein of ‘‘internal’’ cuticles and is expressed in the tracheas, oesophagus and stomach, and also in the embryo. The expression of apd-3 partly overlaps with both apd-1 and apd-3, but apd-3 also is uniquely associated with non-pigmented cuticles such as the eye cover and external cuticle of white pupae. This study expands the collection of genes encoding cuticular proteins by three novel and well characterised members.
Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume37
Issue number2
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

Cite this

Kucharski, R. ; Maleszka, J. ; Maleszka, R. / Novel cuticular proteins revealed by the honey bee genome. In: Insect Biochemistry and Molecular Biology. 2007 ; Vol. 37, No. 2. pp. 128-134.
@article{575221caeea9426e8b4c282cd7866de9,
title = "Novel cuticular proteins revealed by the honey bee genome",
abstract = "With the completion of the honey bee genome project, a transition is now occurring from the acquisition of gene sequence to understanding the role and context of gene products within the genome. Here we annotated and characterised a cluster of three genes in a GC-rich 11 kb genomic region on the linkage group 4 encoding highly hydrophobic polypeptides (named apidermins; APD 1–3) containing both sequence motifs characteristic of cuticular proteins and distinctly novel features. Five amino acids, Ala, Gly, Leu, Pro and Val, account for 74–86{\%} of their respective sequences with Ala being the most abundant residue (at least 30{\%} of each peptide). A conserved tetra-peptide AAPA/V is found in all three proteins, but none has the 0 R and R0 signature implicated in chitin binding. Two proteins, APD-1 and APD-2, contain an arginine-rich motif RERR in short non-hydrophobic stretches near the N-terminal of mature proteins and in both proteins tryptophan is the C-terminal residue. All three genes are spliced and highly expressed in a defined spatiotemporal pattern. apd-1 is expressed in the exoskeletal epidermis, but only during a restricted period of a few days of late pupal and early adult life when the cuticle becomes dark. APD2 appears to be a protein of ‘‘internal’’ cuticles and is expressed in the tracheas, oesophagus and stomach, and also in the embryo. The expression of apd-3 partly overlaps with both apd-1 and apd-3, but apd-3 also is uniquely associated with non-pigmented cuticles such as the eye cover and external cuticle of white pupae. This study expands the collection of genes encoding cuticular proteins by three novel and well characterised members.",
keywords = "Apis mellifera, trachea, genome annotation, flexible cuticle, hydrophobic proteins",
author = "R. Kucharski and J. Maleszka and R. Maleszka",
year = "2007",
month = "2",
doi = "10.1016/j.ibmb.2006.10.009",
language = "English",
volume = "37",
pages = "128--134",
journal = "Insect Biochemistry",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "2",

}

Novel cuticular proteins revealed by the honey bee genome. / Kucharski, R.; Maleszka, J.; Maleszka, R.

In: Insect Biochemistry and Molecular Biology, Vol. 37, No. 2, 02.2007, p. 128-134.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel cuticular proteins revealed by the honey bee genome

AU - Kucharski, R.

AU - Maleszka, J.

AU - Maleszka, R.

PY - 2007/2

Y1 - 2007/2

N2 - With the completion of the honey bee genome project, a transition is now occurring from the acquisition of gene sequence to understanding the role and context of gene products within the genome. Here we annotated and characterised a cluster of three genes in a GC-rich 11 kb genomic region on the linkage group 4 encoding highly hydrophobic polypeptides (named apidermins; APD 1–3) containing both sequence motifs characteristic of cuticular proteins and distinctly novel features. Five amino acids, Ala, Gly, Leu, Pro and Val, account for 74–86% of their respective sequences with Ala being the most abundant residue (at least 30% of each peptide). A conserved tetra-peptide AAPA/V is found in all three proteins, but none has the 0 R and R0 signature implicated in chitin binding. Two proteins, APD-1 and APD-2, contain an arginine-rich motif RERR in short non-hydrophobic stretches near the N-terminal of mature proteins and in both proteins tryptophan is the C-terminal residue. All three genes are spliced and highly expressed in a defined spatiotemporal pattern. apd-1 is expressed in the exoskeletal epidermis, but only during a restricted period of a few days of late pupal and early adult life when the cuticle becomes dark. APD2 appears to be a protein of ‘‘internal’’ cuticles and is expressed in the tracheas, oesophagus and stomach, and also in the embryo. The expression of apd-3 partly overlaps with both apd-1 and apd-3, but apd-3 also is uniquely associated with non-pigmented cuticles such as the eye cover and external cuticle of white pupae. This study expands the collection of genes encoding cuticular proteins by three novel and well characterised members.

AB - With the completion of the honey bee genome project, a transition is now occurring from the acquisition of gene sequence to understanding the role and context of gene products within the genome. Here we annotated and characterised a cluster of three genes in a GC-rich 11 kb genomic region on the linkage group 4 encoding highly hydrophobic polypeptides (named apidermins; APD 1–3) containing both sequence motifs characteristic of cuticular proteins and distinctly novel features. Five amino acids, Ala, Gly, Leu, Pro and Val, account for 74–86% of their respective sequences with Ala being the most abundant residue (at least 30% of each peptide). A conserved tetra-peptide AAPA/V is found in all three proteins, but none has the 0 R and R0 signature implicated in chitin binding. Two proteins, APD-1 and APD-2, contain an arginine-rich motif RERR in short non-hydrophobic stretches near the N-terminal of mature proteins and in both proteins tryptophan is the C-terminal residue. All three genes are spliced and highly expressed in a defined spatiotemporal pattern. apd-1 is expressed in the exoskeletal epidermis, but only during a restricted period of a few days of late pupal and early adult life when the cuticle becomes dark. APD2 appears to be a protein of ‘‘internal’’ cuticles and is expressed in the tracheas, oesophagus and stomach, and also in the embryo. The expression of apd-3 partly overlaps with both apd-1 and apd-3, but apd-3 also is uniquely associated with non-pigmented cuticles such as the eye cover and external cuticle of white pupae. This study expands the collection of genes encoding cuticular proteins by three novel and well characterised members.

KW - Apis mellifera

KW - trachea

KW - genome annotation

KW - flexible cuticle

KW - hydrophobic proteins

U2 - 10.1016/j.ibmb.2006.10.009

DO - 10.1016/j.ibmb.2006.10.009

M3 - Article

VL - 37

SP - 128

EP - 134

JO - Insect Biochemistry

JF - Insect Biochemistry

SN - 0965-1748

IS - 2

ER -