TY - JOUR
T1 - Novel microsatellite markers obtained from Gansu zokor (Eospalax cansus) and cross-species amplification in Plateau zokor (Eospalax baileyi)
AU - Su, Junhu
AU - Ji, Weihong
AU - Howitt, Robyn
AU - Hua, Limin
AU - GLEESON, Dianne
AU - Duan, Huailong
PY - 2014/12
Y1 - 2014/12
N2 - Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.
AB - Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.
KW - Zokor
KW - Mysopalacinae
KW - Microsatellite
KW - Polymorphism
KW - Cross-species amplification.
KW - Cross-species amplification
UR - http://www.scopus.com/inward/record.url?scp=84907324387&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/novel-microsatellite-markers-obtained-gansu-zokor-eospalax-cansus-crossspecies-amplification-plateau
U2 - 10.1016/j.bse.2014.07.017
DO - 10.1016/j.bse.2014.07.017
M3 - Article
SN - 0305-1978
VL - 57
SP - 128
EP - 132
JO - Biochemical Systematics and Ecology
JF - Biochemical Systematics and Ecology
ER -