Novel microsatellite markers obtained from Gansu zokor (Eospalax cansus) and cross-species amplification in Plateau zokor (Eospalax baileyi)

Junhu Su, Weihong Ji, Robyn Howitt, Limin Hua, Dianne GLEESON, Huailong Duan

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.
    Original languageEnglish
    Pages (from-to)128-132
    Number of pages5
    JournalBiochemical Systematics and Ecology
    Volume57
    DOIs
    Publication statusPublished - 2014

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    Microsatellite Repeats
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    microsatellite repeats
    China
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    Cite this

    @article{b86350ff5165446eb7a07692015002aa,
    title = "Novel microsatellite markers obtained from Gansu zokor (Eospalax cansus) and cross-species amplification in Plateau zokor (Eospalax baileyi)",
    abstract = "Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.",
    keywords = "Zokor, Mysopalacinae, Microsatellite, Polymorphism, Cross-species amplification.",
    author = "Junhu Su and Weihong Ji and Robyn Howitt and Limin Hua and Dianne GLEESON and Huailong Duan",
    year = "2014",
    doi = "10.1016/j.bse.2014.07.017",
    language = "English",
    volume = "57",
    pages = "128--132",
    journal = "Biochemical Systematics and Ecology",
    issn = "0305-1978",
    publisher = "Elsevier Limited",

    }

    Novel microsatellite markers obtained from Gansu zokor (Eospalax cansus) and cross-species amplification in Plateau zokor (Eospalax baileyi). / Su, Junhu; Ji, Weihong; Howitt, Robyn; Hua, Limin; GLEESON, Dianne; Duan, Huailong.

    In: Biochemical Systematics and Ecology, Vol. 57, 2014, p. 128-132.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Novel microsatellite markers obtained from Gansu zokor (Eospalax cansus) and cross-species amplification in Plateau zokor (Eospalax baileyi)

    AU - Su, Junhu

    AU - Ji, Weihong

    AU - Howitt, Robyn

    AU - Hua, Limin

    AU - GLEESON, Dianne

    AU - Duan, Huailong

    PY - 2014

    Y1 - 2014

    N2 - Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.

    AB - Zokor (Mysopalacinae) is a group of subterranean rodents. Although they are regarded as important components in the ecosystems they belong to, due to their underground lifestyle, very little is known about their ecology and behavior. Development of microsatellite markers can potentially assist in addressing behavioral and ecological questions such as dispersal, mating and social systems. Here we report the development of 11 polymorphic microsatellite loci from the genome of Eospalax cansus using 454 shot-gun sequencing. Genetic diversity was assessed using DNA samples of 47 individuals of E. cansus from a single location. Cross-amplification in Eospalax baileyi was also tested, with five polymorphic markers being amplified successfully. These markers are the first published microsatellites loci from E. cansus and will be invaluable for studies addressing ecological and behavioral questions involving E. cansus and E. baileyi, and potentially other species in Mysopalacinae.

    KW - Zokor

    KW - Mysopalacinae

    KW - Microsatellite

    KW - Polymorphism

    KW - Cross-species amplification.

    U2 - 10.1016/j.bse.2014.07.017

    DO - 10.1016/j.bse.2014.07.017

    M3 - Article

    VL - 57

    SP - 128

    EP - 132

    JO - Biochemical Systematics and Ecology

    JF - Biochemical Systematics and Ecology

    SN - 0305-1978

    ER -