Nuclear and mitochondrial DNA variation within threatened species and subspecies of the giant New Zealand land snail genus Powelliphanta: implications for classification and conservation

Thomas Buckley, Daniel White, Robyn Howitt, Tom Winstanley, Ana Ramón-Laca, Dianne GLEESON

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    We developed eight microsatellite markers using high-throughput pyrosequencing and screened these in two species (82 individuals) of threatened New Zealand land snails from the genus Powelliphanta. The taxa examined included five of the seven subspecies of P. lignaria, in addition to its sister species, the newly described P. augusta. We also sequenced part of the cytochrome c oxidase subunit I gene for these taxa. Powelliphanta augusta is differentiated from its sister species P. lignaria at both mitochondrial DNA and microsatellite loci. Of the five P. lignaria subspecies we sampled, only one formed an exclusive genetic cluster based on Bayesian clustering of microsatellite data. None of the P. lignaria subspecies was monophyletic for mitochondrial DNA. We are unable to determine if the lack of genetic differentiation is the result of hybridization, as hypothesized by previous authors, or very recent differentiation. Our data cast doubt on the current classification of subspecies within P. lignaria and suggest that further scrutiny of the current morphological characters used to differentiate these subspecies is warranted. We recommend that conservation strategies be based on genetically defined groups identified through analysis of multiple nuclear markers rather than the existing taxonomic subspecies of P. lignaria.
    Original languageEnglish
    Pages (from-to)291-302
    Number of pages12
    JournalJournal of Molluscan Studies
    Volume80
    Issue number3
    DOIs
    Publication statusPublished - 2014

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    threatened species
    mitochondrial DNA
    nuclear genome
    snail
    subspecies
    snails
    microsatellite repeats
    taxonomy
    cytochrome-c oxidase
    hybridization
    genetic variation
    loci
    genetic differentiation
    cytochrome
    land
    genes
    gene

    Cite this

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    title = "Nuclear and mitochondrial DNA variation within threatened species and subspecies of the giant New Zealand land snail genus Powelliphanta: implications for classification and conservation",
    abstract = "We developed eight microsatellite markers using high-throughput pyrosequencing and screened these in two species (82 individuals) of threatened New Zealand land snails from the genus Powelliphanta. The taxa examined included five of the seven subspecies of P. lignaria, in addition to its sister species, the newly described P. augusta. We also sequenced part of the cytochrome c oxidase subunit I gene for these taxa. Powelliphanta augusta is differentiated from its sister species P. lignaria at both mitochondrial DNA and microsatellite loci. Of the five P. lignaria subspecies we sampled, only one formed an exclusive genetic cluster based on Bayesian clustering of microsatellite data. None of the P. lignaria subspecies was monophyletic for mitochondrial DNA. We are unable to determine if the lack of genetic differentiation is the result of hybridization, as hypothesized by previous authors, or very recent differentiation. Our data cast doubt on the current classification of subspecies within P. lignaria and suggest that further scrutiny of the current morphological characters used to differentiate these subspecies is warranted. We recommend that conservation strategies be based on genetically defined groups identified through analysis of multiple nuclear markers rather than the existing taxonomic subspecies of P. lignaria.",
    author = "Thomas Buckley and Daniel White and Robyn Howitt and Tom Winstanley and Ana Ram{\'o}n-Laca and Dianne GLEESON",
    year = "2014",
    doi = "10.1093/mollus/eyu014",
    language = "English",
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    Nuclear and mitochondrial DNA variation within threatened species and subspecies of the giant New Zealand land snail genus Powelliphanta: implications for classification and conservation. / Buckley, Thomas; White, Daniel; Howitt, Robyn; Winstanley, Tom; Ramón-Laca, Ana; GLEESON, Dianne.

    In: Journal of Molluscan Studies, Vol. 80, No. 3, 2014, p. 291-302.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Nuclear and mitochondrial DNA variation within threatened species and subspecies of the giant New Zealand land snail genus Powelliphanta: implications for classification and conservation

    AU - Buckley, Thomas

    AU - White, Daniel

    AU - Howitt, Robyn

    AU - Winstanley, Tom

    AU - Ramón-Laca, Ana

    AU - GLEESON, Dianne

    PY - 2014

    Y1 - 2014

    N2 - We developed eight microsatellite markers using high-throughput pyrosequencing and screened these in two species (82 individuals) of threatened New Zealand land snails from the genus Powelliphanta. The taxa examined included five of the seven subspecies of P. lignaria, in addition to its sister species, the newly described P. augusta. We also sequenced part of the cytochrome c oxidase subunit I gene for these taxa. Powelliphanta augusta is differentiated from its sister species P. lignaria at both mitochondrial DNA and microsatellite loci. Of the five P. lignaria subspecies we sampled, only one formed an exclusive genetic cluster based on Bayesian clustering of microsatellite data. None of the P. lignaria subspecies was monophyletic for mitochondrial DNA. We are unable to determine if the lack of genetic differentiation is the result of hybridization, as hypothesized by previous authors, or very recent differentiation. Our data cast doubt on the current classification of subspecies within P. lignaria and suggest that further scrutiny of the current morphological characters used to differentiate these subspecies is warranted. We recommend that conservation strategies be based on genetically defined groups identified through analysis of multiple nuclear markers rather than the existing taxonomic subspecies of P. lignaria.

    AB - We developed eight microsatellite markers using high-throughput pyrosequencing and screened these in two species (82 individuals) of threatened New Zealand land snails from the genus Powelliphanta. The taxa examined included five of the seven subspecies of P. lignaria, in addition to its sister species, the newly described P. augusta. We also sequenced part of the cytochrome c oxidase subunit I gene for these taxa. Powelliphanta augusta is differentiated from its sister species P. lignaria at both mitochondrial DNA and microsatellite loci. Of the five P. lignaria subspecies we sampled, only one formed an exclusive genetic cluster based on Bayesian clustering of microsatellite data. None of the P. lignaria subspecies was monophyletic for mitochondrial DNA. We are unable to determine if the lack of genetic differentiation is the result of hybridization, as hypothesized by previous authors, or very recent differentiation. Our data cast doubt on the current classification of subspecies within P. lignaria and suggest that further scrutiny of the current morphological characters used to differentiate these subspecies is warranted. We recommend that conservation strategies be based on genetically defined groups identified through analysis of multiple nuclear markers rather than the existing taxonomic subspecies of P. lignaria.

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