On the enantioselective HPLC separation ability of sub-2 µm columns

Chiralpak ® Ig-U and Id-U

Diana Ibrahim, Ashraf Ghanem

Research output: Contribution to journalArticle

Abstract

Silica with a particle size of 3–5 µm has been widely used as selector backbone material in 10–25 cm HPLC chiral columns. Yet, with the availability of 1.6 µm particles, shorter, high-efficiency columns practical for minute chiral separations are possible to fabricate. Herein, we investigate the use of two recently commercialized sub-2 µm columns with different substituents. Thus, Chiralpak ® IG-U and ID-U were used in HPLC for the fast enantioseparation of a set of drugs. Chiralpak ® IG-U [amylose tris (3-chloro-5-methylphenylcarbamate)] has two substituents on the phenyl ring, namely, a withdrawing chlorine group in the third position and a donating group in the fifth position. Chiralpak ® ID-U [amylose tris (3-chlorophenylcarbamate)] has only one substituent on the phenyl ring, namely a withdrawing chlorine group. Their applications in three liquid chromatography modes, namely, normal phase, polar organic mode, and reversed phase, were demonstrated. Both columns have similar column parameters (50 mm length, 3 mm internal diameter, and 1.6 µm particle size) with the chiral stationary phase as the only variable. Improved chromatographic enantioresolution was obtained with Chiralpak ® ID-U. Amino acids partially separated were reported for the first time under an amylose-based sub-2-micron column.

Original languageEnglish
Article number1287
Pages (from-to)1-11
Number of pages11
JournalMolecules
Volume24
Issue number7
DOIs
Publication statusPublished - 2 Apr 2019

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Amylose
Chlorine
High Pressure Liquid Chromatography
Particle Size
Particle size
Liquid chromatography
Liquid Chromatography
Silicon Dioxide
chlorine
Availability
Amino Acids
selectors
rings
liquid chromatography
Pharmaceutical Preparations
availability
amino acids
drugs
silicon dioxide

Cite this

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title = "On the enantioselective HPLC separation ability of sub-2 µm columns: Chiralpak {\circledR} Ig-U and Id-U",
abstract = "Silica with a particle size of 3–5 µm has been widely used as selector backbone material in 10–25 cm HPLC chiral columns. Yet, with the availability of 1.6 µm particles, shorter, high-efficiency columns practical for minute chiral separations are possible to fabricate. Herein, we investigate the use of two recently commercialized sub-2 µm columns with different substituents. Thus, Chiralpak {\circledR} IG-U and ID-U were used in HPLC for the fast enantioseparation of a set of drugs. Chiralpak {\circledR} IG-U [amylose tris (3-chloro-5-methylphenylcarbamate)] has two substituents on the phenyl ring, namely, a withdrawing chlorine group in the third position and a donating group in the fifth position. Chiralpak {\circledR} ID-U [amylose tris (3-chlorophenylcarbamate)] has only one substituent on the phenyl ring, namely a withdrawing chlorine group. Their applications in three liquid chromatography modes, namely, normal phase, polar organic mode, and reversed phase, were demonstrated. Both columns have similar column parameters (50 mm length, 3 mm internal diameter, and 1.6 µm particle size) with the chiral stationary phase as the only variable. Improved chromatographic enantioresolution was obtained with Chiralpak {\circledR} ID-U. Amino acids partially separated were reported for the first time under an amylose-based sub-2-micron column.",
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author = "Diana Ibrahim and Ashraf Ghanem",
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On the enantioselective HPLC separation ability of sub-2 µm columns : Chiralpak ® Ig-U and Id-U. / Ibrahim, Diana; Ghanem, Ashraf.

In: Molecules, Vol. 24, No. 7, 1287, 02.04.2019, p. 1-11.

Research output: Contribution to journalArticle

TY - JOUR

T1 - On the enantioselective HPLC separation ability of sub-2 µm columns

T2 - Chiralpak ® Ig-U and Id-U

AU - Ibrahim, Diana

AU - Ghanem, Ashraf

PY - 2019/4/2

Y1 - 2019/4/2

N2 - Silica with a particle size of 3–5 µm has been widely used as selector backbone material in 10–25 cm HPLC chiral columns. Yet, with the availability of 1.6 µm particles, shorter, high-efficiency columns practical for minute chiral separations are possible to fabricate. Herein, we investigate the use of two recently commercialized sub-2 µm columns with different substituents. Thus, Chiralpak ® IG-U and ID-U were used in HPLC for the fast enantioseparation of a set of drugs. Chiralpak ® IG-U [amylose tris (3-chloro-5-methylphenylcarbamate)] has two substituents on the phenyl ring, namely, a withdrawing chlorine group in the third position and a donating group in the fifth position. Chiralpak ® ID-U [amylose tris (3-chlorophenylcarbamate)] has only one substituent on the phenyl ring, namely a withdrawing chlorine group. Their applications in three liquid chromatography modes, namely, normal phase, polar organic mode, and reversed phase, were demonstrated. Both columns have similar column parameters (50 mm length, 3 mm internal diameter, and 1.6 µm particle size) with the chiral stationary phase as the only variable. Improved chromatographic enantioresolution was obtained with Chiralpak ® ID-U. Amino acids partially separated were reported for the first time under an amylose-based sub-2-micron column.

AB - Silica with a particle size of 3–5 µm has been widely used as selector backbone material in 10–25 cm HPLC chiral columns. Yet, with the availability of 1.6 µm particles, shorter, high-efficiency columns practical for minute chiral separations are possible to fabricate. Herein, we investigate the use of two recently commercialized sub-2 µm columns with different substituents. Thus, Chiralpak ® IG-U and ID-U were used in HPLC for the fast enantioseparation of a set of drugs. Chiralpak ® IG-U [amylose tris (3-chloro-5-methylphenylcarbamate)] has two substituents on the phenyl ring, namely, a withdrawing chlorine group in the third position and a donating group in the fifth position. Chiralpak ® ID-U [amylose tris (3-chlorophenylcarbamate)] has only one substituent on the phenyl ring, namely a withdrawing chlorine group. Their applications in three liquid chromatography modes, namely, normal phase, polar organic mode, and reversed phase, were demonstrated. Both columns have similar column parameters (50 mm length, 3 mm internal diameter, and 1.6 µm particle size) with the chiral stationary phase as the only variable. Improved chromatographic enantioresolution was obtained with Chiralpak ® ID-U. Amino acids partially separated were reported for the first time under an amylose-based sub-2-micron column.

KW - Chiralpak ID-U

KW - Chiralpak IG-U

KW - Mobile phase modifiers

KW - Polar organic and reversed phase modes

KW - Sub-2 µm particles

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DO - 10.3390/molecules24071287

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VL - 24

SP - 1

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JO - Molecules

JF - Molecules

SN - 1420-3049

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M1 - 1287

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